CAJ | 학술논문

目的通过动物实验检测动物血清中IL-6、TNF-α的含量变化及头颅骨中的破骨细胞的表达情况,评价云南白药对牙龈卟啉单胞菌(P.g)诱导骨破坏的影响.方法选用雄性昆明小鼠72只,用随机数字法分为模型组、白药治疗组和对照组.模型组和云南白药治疗组在小鼠两耳中间颅顶至枕部骨的骨上,接种P.g 1×108 cfu/ml,200μl,分2次隔日注射,对照组则注射同等剂量的生理盐水.同时云南白药治疗组在建模的当天开始给予云南白药灌胃每只0.5 ml(12.5 g/L),模型组和对照组则给予同等剂量的生理盐水灌胃.在建模的第5天、第8天、第14天分批各组各处死8只.运用ELISA方法检测各组中血清TNF-α、IL-6的含量变化;抗酒石酸酸性磷酸酶染色(TRAP)染色法检测头颅骨中破骨细胞的含量.结果分别在第5天、第8天和第14天,对3组小鼠血清中TNF-α、IL-6的含量和破骨细胞的数目进行两两比较,发现模型组明显高于云南白药治疗组(P＜0.05),且在第5天达到高峰,TNF-α为(621.18±29.55)μg/L,IL-6为(79.15±3.12)μg/L,破骨细胞为5.61±0.24;同时两组和对照组相比也高于对照组(P＜0.05).结论云南白药有明显抑制骨破坏的作用,其机制可能与降低IL-6、TNF-α的表达,抑制破骨细胞的形成,从而抑制骨的吸收有关.
목적 통과동물실험검측동물혈청중IL-6、TNF-α적함량변화급두로골중적파골세포적표체정황,평개운남백약대아간계람단포균(P.g)유도골파배적영향.방법 선용웅성곤명소서72지,용수궤수자법분위모형조、백약치료조화대조조.모형조화운남백약치료조재소서량이중간로정지침부골적골상,접충P.g 1×108 cfu/ml,200μl,분2차격일주사,대조조칙주사동등제량적생리염수.동시운남백약치료조재건모적당천개시급여운남백약관위매지0.5 ml(12.5 g/L),모형조화대조조칙급여동등제량적생리염수관위.재건모적제5천、제8천、제14천분비각조각처사8지.운용ELISA방법검측각조중혈청TNF-α、IL-6적함량변화;항주석산산성린산매염색(TRAP)염색법검측두로골중파골세포적함량.결과 분별재제5천、제8천화제14천,대3조소서혈청중TNF-α、IL-6적함량화파골세포적수목진행량량비교,발현모형조명현고우운남백약치료조(P＜0.05),차재제5천체도고봉,TNF-α위(621.18±29.55)μg/L,IL-6위(79.15±3.12)μg/L,파골세포위5.61±0.24;동시량조화대조조상비야고우대조조(P＜0.05).결론 운남백약유명현억제골파배적작용,기궤제가능여강저IL-6、TNF-α적표체,억제파골세포적형성,종이억제골적흡수유관.
Objective To evaluate the effects of Yunnan Baiyao on Porphyromonas gingivalis (P.gingivalis)-induced bone destruction by studying the change of IL-6 and TNF-α expression and osteoclast count in skull in mice.Methods Seventy-two male Kunming mice were randomly assigned to inoculation with P.gingivalis (1 × 108 cfu/ml,200 μl) between the calvarium and occiput region at the midline of both ears,twice in two consecutive days (model group) and followed by gastric administration of Yunnan Baiyao (12.5 g/ml for 0.5 ml,treatment group),as well as inoculation with 200 μl normal saline followed by gastric administration of 0.5 ml saline (control group),respectively.Eight mice were sacrificed at days 5,8 and 14 after inoculation.Enzyme-linked immunosorbent assay was employed to detect the changes in serum IL-6 and TNF-α,and tartrate-resistant acid phosphatase staining was adopted to measure the calvarium osteoclast count.Results The model group yielded considerably higher levels of IL-6 and TNF-α and osteoclast count at days 5,8 and 14,which culminated at day 5 [(621.18±29.55)μg/L for TNF-α,(79.15±3.12)μg/L for IL-6 and 5.61±0.24 for osteoclast count],when compared with treatment group (all P＜0.05).Both model and treatment groups had higher levels of all indices than control group (all P＜0.05).Conclusion The significant suppression of Yunnan Baiyao on bone destruction,as evidenced by attenuated IL-6 and TNF-α expression,may inhibit osteoclast formation and bone absorption.