CAJ | 학술논문

目的研究锌指蛋白36(ZFP36)基因多态性与新疆维吾尔族人群肥胖的相关性.方法采用整群随机抽样方法抽取932例维吾尔族人群作为研究对象,检测其表型资料.在48例维吾尔族肥胖人群中测序筛查ZFP36基因功能区的变异位点,选取代表性变异位点.应用TaqMan-PCR在大样本人群中进行基因型鉴定及病例-对照关联研究.结果 ZFP36基因rs3746083位点CC、CT、TT基因型及C、T等位基因在按体质量指数(BMI)分组的肥胖、超重、正常组分布频率差异无统计学意义(P＞0.05).按腰围分组的腹型肥胖组和正常组之间该位点3种基因型分布频率差异无统计学意义(P＞0.05),但其等位基因分布频率差异有统计学意义(x2=14.314,P＜0.05).在男性腹型肥胖组和正常组间,rs3746083位点CC、CT、TT基因型及C、T等位基因分布频率差异有统计学意义(x2=6.264,x2=8.307,P＜0.05),但在女性差异却无统计学意义(P＞0.05).rs3746083位点CT+TT基因型与CC基因型之间与空腹血糖浓度及胰岛素抵抗指数差异有统计学意义(P＜0.05).rs251864基因型在男性腹型肥胖组和正常组之间的AA、AG、GG种基因型频率差异有统计学意义(P＜0.05),但在女性两组之间rs251864 3种基因型及等位基因频率差异无统计学意义(P＞0.05).结论 ZFP36基因rs3746083变异位点与维吾尔族男性腹型肥胖可能有相关性.
목적 연구자지단백36(ZFP36)기인다태성여신강유오이족인군비반적상관성.방법 채용정군수궤추양방법추취932례유오이족인군작위연구대상,검측기표형자료.재48례유오이족비반인군중측서사사ZFP36기인공능구적변이위점,선취대표성변이위점.응용TaqMan-PCR재대양본인군중진행기인형감정급병례-대조관련연구.결과 ZFP36기인rs3746083위점CC、CT、TT기인형급C、T등위기인재안체질량지수(BMI)분조적비반、초중、정상조분포빈솔차이무통계학의의(P＞0.05).안요위분조적복형비반조화정상조지간해위점3충기인형분포빈솔차이무통계학의의(P＞0.05),단기등위기인분포빈솔차이유통계학의의(x2=14.314,P＜0.05).재남성복형비반조화정상조간,rs3746083위점CC、CT、TT기인형급C、T등위기인분포빈솔차이유통계학의의(x2=6.264,x2=8.307,P＜0.05),단재녀성차이각무통계학의의(P＞0.05).rs3746083위점CT+TT기인형여CC기인형지간여공복혈당농도급이도소저항지수차이유통계학의의(P＜0.05).rs251864기인형재남성복형비반조화정상조지간적AA、AG、GG충기인형빈솔차이유통계학의의(P＜0.05),단재녀성량조지간rs251864 3충기인형급등위기인빈솔차이무통계학의의(P＞0.05).결론 ZFP36기인rs3746083변이위점여유오이족남성복형비반가능유상관성.
Objective To investigate the association of varying polymorphisms of zinc finger protein-36 (ZFP36) and obesity in Xinjiang Uygur population.Methods A total of 932 Uygur individuals were enrolled in the study with random clustered sampling for assessment of the genotypes.Following screening of the mutant points of ZFP36 gene by sequencing in 48 Uygur individuals with obesity,the representative points were selected for subsequent genotyping and case-control study by using Taqman-PCR on the basis of an extended population.Results The difference in distribution of individuals with obesity,overweight and normal controls,based on body mass index (BMI),did not reach statistical significance for genotypes CC,CT and TT and alleles C and T corresponding to rs3746083 of ZFP36 gene (P＞0.05).No marked difference in the distribution of three genotypes (P＞0.05),but not that of the alleles (x2=14.314,P＜0.05),was noted in individuals with abdominal obesity and normal waist circumference on the basis of waist circumference.The distribution of frequency of genotypes CC,CT and TT and alleles C and T for rs3746083 differed considerably between males with obesity and normal waist circumference (x2=6.264,x2=8.307,P＜0.05,respectively),which was absent in females (P＞0.05).The genotype profiles suggested that the differences in fasting blood glucose and insulin resistance for rs3746083 between those with genotypes CT+ TT and CC were statistically significant (P＜0.05).When grouped on the basis of BMI,the genotype distribution frequency of AA,AG and GG and alleles A and G for rs251864 in individuals with obesity,overweight and normal controls did not appear statistically significant (P＞0.05).There was no profound distinction in the genotype distribution frequency of all genotypes and alleles for rs251864,on the basis of waist circumference,in individuals with abdominal obesity and normal waist circumference (P＞0.05).The AA,AG and GG genotypes,but not the corresponding alleles,yielded a marked difference in rs251864 between males with abdominal obesity and normal waist circumference (P＜0.05).Nonetheless,this distinction in females with abdominal obesity and normal waist circumference did not reach statistical significance (P＞0.05).There was a marked difference in the diastolic blood pressure among patients with genotypes AA,AG and GG of rs251864 (P＜0.05).Conclusion The zinc-finger protein-36 rs3746083 polymorphisms may be associated with abdominal obesity in Uygur males.