中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2013年
2期
117-119
,共3页
蒲蜀湘%饶从磊%梁兵%袁芳%吴培基%杨宁%高聪
蒲蜀湘%饒從磊%樑兵%袁芳%吳培基%楊寧%高聰
포촉상%요종뢰%량병%원방%오배기%양저%고총
帕金森病%活性氧%过氧化氢%多巴胺能神经元%SH-SY5Y细胞%细胞增殖
帕金森病%活性氧%過氧化氫%多巴胺能神經元%SH-SY5Y細胞%細胞增殖
파금삼병%활성양%과양화경%다파알능신경원%SH-SY5Y세포%세포증식
Parkinson disease%Reactive oxygen species%Hydrogen peroxide%Dopaminergic neurons%SH-SY5Y cells%Cell proliferation
目的 探讨低浓度活性氧对多巴胺能神经元增殖的影响.方法 实验组分别用低浓度(10、20和30 μmol/L)过氧化氢(H2O2)干预人神经母细胞瘤细胞SH-SY5Y 24 h,对照组加入等体积培养基.应用MTT法检测各组细胞活性;比色法测定各组细胞乳酸脱氢酶(LDH)释放率.结果 低浓度H2O2干预SH-SY5Y细胞24 h后,10 μmol/L H2O2组、20 μmol/L H2O2组和30 μmol/L H2O2组吸光度A570值分别为1.011 2±0.015 3、1.119 1±0.035 3和1.027 1±0.035 2,与对照组(0.959 0±0.034 7)比较,差异均有统计学意义(均P＜0.05);10、20和30 μmol/L H2O2组之间两两比较,差异均有统计学意义(F=18.727,P＜0.05),其中20 μmol/L H2O2组促进SH-SY5Y细胞增殖效果最为明显.10、20和30 μmol/L H2O2组LDH释放率分别为(26.00±1.09)％、(24.62±0.77)％、(25.88±0.95)％,与对照组LDH释放率(27.86±1.31)％相比,差异均具有统计学意义(均P＜0.05);10、20和30 μmol/L H2O2组之间两两比较,差异无统计学意义(F=3.254,P=0.074).结论 低浓度活性氧可能对多巴胺能神经元的增殖有促进作用.
目的 探討低濃度活性氧對多巴胺能神經元增殖的影響.方法 實驗組分彆用低濃度(10、20和30 μmol/L)過氧化氫(H2O2)榦預人神經母細胞瘤細胞SH-SY5Y 24 h,對照組加入等體積培養基.應用MTT法檢測各組細胞活性;比色法測定各組細胞乳痠脫氫酶(LDH)釋放率.結果 低濃度H2O2榦預SH-SY5Y細胞24 h後,10 μmol/L H2O2組、20 μmol/L H2O2組和30 μmol/L H2O2組吸光度A570值分彆為1.011 2±0.015 3、1.119 1±0.035 3和1.027 1±0.035 2,與對照組(0.959 0±0.034 7)比較,差異均有統計學意義(均P＜0.05);10、20和30 μmol/L H2O2組之間兩兩比較,差異均有統計學意義(F=18.727,P＜0.05),其中20 μmol/L H2O2組促進SH-SY5Y細胞增殖效果最為明顯.10、20和30 μmol/L H2O2組LDH釋放率分彆為(26.00±1.09)％、(24.62±0.77)％、(25.88±0.95)％,與對照組LDH釋放率(27.86±1.31)％相比,差異均具有統計學意義(均P＜0.05);10、20和30 μmol/L H2O2組之間兩兩比較,差異無統計學意義(F=3.254,P=0.074).結論 低濃度活性氧可能對多巴胺能神經元的增殖有促進作用.
목적 탐토저농도활성양대다파알능신경원증식적영향.방법 실험조분별용저농도(10、20화30 μmol/L)과양화경(H2O2)간예인신경모세포류세포SH-SY5Y 24 h,대조조가입등체적배양기.응용MTT법검측각조세포활성;비색법측정각조세포유산탈경매(LDH)석방솔.결과 저농도H2O2간예SH-SY5Y세포24 h후,10 μmol/L H2O2조、20 μmol/L H2O2조화30 μmol/L H2O2조흡광도A570치분별위1.011 2±0.015 3、1.119 1±0.035 3화1.027 1±0.035 2,여대조조(0.959 0±0.034 7)비교,차이균유통계학의의(균P＜0.05);10、20화30 μmol/L H2O2조지간량량비교,차이균유통계학의의(F=18.727,P＜0.05),기중20 μmol/L H2O2조촉진SH-SY5Y세포증식효과최위명현.10、20화30 μmol/L H2O2조LDH석방솔분별위(26.00±1.09)％、(24.62±0.77)％、(25.88±0.95)％,여대조조LDH석방솔(27.86±1.31)％상비,차이균구유통계학의의(균P＜0.05);10、20화30 μmol/L H2O2조지간량량비교,차이무통계학의의(F=3.254,P=0.074).결론 저농도활성양가능대다파알능신경원적증식유촉진작용.
Objective To investigate the effects of low concentrations of reactive oxygen species on dopaminergic neuron proliferation.Methods The human neuroblastoma cells SH-SY5Y were incubated with low concentrations (10,20 and 30 μmol/L) of H2O2 for 24 h in the study group and an aliquot of culture media in the blank control group,respectively.The viability of SH-SY5Y cells was assayed by the MTT method,and the release of LDH was determined by the colorimetric assay.Results Following treatment with low concentrations of H2O2 for 24 h,the SH-SY5Y cells yielded a significantly higher absorption (A570)1.011 2±0.015 3,1.119 1±0.035 3 and 1.027 1±0.035 2 corresponding to 10,20 and 30 μmol/L H2O2 when compared with control group 0.959 0± 0.034 7 (all P＜0.05) respectively.The between-group difference in study groups was statistically significant (F=18.727,P＜0.05).The 20 μ mol/L group showed the most significant effect on cell proliferation.Treatment with 10,20 and 30 μmol/L H2O2 was characterized by considerably higher release of LDH (26.00± 1.09)％,(24.62±0.77)％ and (25.88±0.95)％ compared with control group (27.86± 1.31)％ (all P＜0.05).However,there was no between-group difference in the release of LDH among the study groups (F=3.254,P=0.074).Conclusion Low concentrations of reactive oxygen species may promote dopaminergic neuron proliferation.
