中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2013年
2期
120-122
,共3页
微RNAs%胃肿瘤%肿瘤转移%miR-10a
微RNAs%胃腫瘤%腫瘤轉移%miR-10a
미RNAs%위종류%종류전이%miR-10a
MicroRNAs%Stomach neoplasms%Neoplasm metastasis%miR-10a
目的 探讨miR-10a在胃癌组织中的表达及与临床病理特征的相关性.方法 采用茎环实时RT-PCR方法检测92例胃癌组织及20例正常胃组织中miR-10a的表达,分析miR-10a的表达与胃癌常见临床病理特征的关系.结果 用茎环实时RT-PCR方法检测miR-10a表达的敏感性和特异性良好.miR-10a在胃癌组织中的表达(11.073±1.874)高于正常胃组织(1.083±0.126),差异有统计学意义(P＜0.01).miR-10a在有淋巴结转移的胃癌组织中的表达(12.893±1.946)高于无淋巴结转移的胃组织(8.249±1.757,P＜0.001);在TNM分期Ⅲ～Ⅳ期胃癌组织中的表达高于Ⅰ～Ⅱ期,差异有统计学意义(P＜0.01).miR-10a的表达与性别、年龄、肿瘤分化程度无显著关系(P＞0.05).结论 miR-10a在胃癌组织中的表达高于正常组织,与胃癌淋巴结转移和临床分期有一定关联.miR-10a可能在胃癌的发展过程中发挥重要作用,有望成为胃癌治疗的新靶点.
目的 探討miR-10a在胃癌組織中的錶達及與臨床病理特徵的相關性.方法 採用莖環實時RT-PCR方法檢測92例胃癌組織及20例正常胃組織中miR-10a的錶達,分析miR-10a的錶達與胃癌常見臨床病理特徵的關繫.結果 用莖環實時RT-PCR方法檢測miR-10a錶達的敏感性和特異性良好.miR-10a在胃癌組織中的錶達(11.073±1.874)高于正常胃組織(1.083±0.126),差異有統計學意義(P＜0.01).miR-10a在有淋巴結轉移的胃癌組織中的錶達(12.893±1.946)高于無淋巴結轉移的胃組織(8.249±1.757,P＜0.001);在TNM分期Ⅲ～Ⅳ期胃癌組織中的錶達高于Ⅰ～Ⅱ期,差異有統計學意義(P＜0.01).miR-10a的錶達與性彆、年齡、腫瘤分化程度無顯著關繫(P＞0.05).結論 miR-10a在胃癌組織中的錶達高于正常組織,與胃癌淋巴結轉移和臨床分期有一定關聯.miR-10a可能在胃癌的髮展過程中髮揮重要作用,有望成為胃癌治療的新靶點.
목적 탐토miR-10a재위암조직중적표체급여림상병리특정적상관성.방법 채용경배실시RT-PCR방법검측92례위암조직급20례정상위조직중miR-10a적표체,분석miR-10a적표체여위암상견림상병리특정적관계.결과 용경배실시RT-PCR방법검측miR-10a표체적민감성화특이성량호.miR-10a재위암조직중적표체(11.073±1.874)고우정상위조직(1.083±0.126),차이유통계학의의(P＜0.01).miR-10a재유림파결전이적위암조직중적표체(12.893±1.946)고우무림파결전이적위조직(8.249±1.757,P＜0.001);재TNM분기Ⅲ～Ⅳ기위암조직중적표체고우Ⅰ～Ⅱ기,차이유통계학의의(P＜0.01).miR-10a적표체여성별、년령、종류분화정도무현저관계(P＞0.05).결론 miR-10a재위암조직중적표체고우정상조직,여위암림파결전이화림상분기유일정관련.miR-10a가능재위암적발전과정중발휘중요작용,유망성위위암치료적신파점.
Objective To explore the association between the miR-10a expression in gastric cancer tissues and the clinicopathologic features of gastric cancer.Methods Expression of miR-10a in 92 specimens of gastric cancer tissue and 20 specimens of normal gastric tissue was examined by stem-loop realtime RT-PCR.The association between miR-10a expression level and clinicopathologic features of gastric cancer was analyzed.Results The stem-loop real-time RT-PCR was sensitive and specific in miR-10a detection.The expression of miR-10a in gastric cancer tissues (11.073± 1.874) was significantly higher than that in normal gastric tissues (1.083 ± 0.126) (P＜0.01),significantly higher in gastric cancer tissues with lymph node metastases (12.893± 1.946) than that in those without lymph node metastases (8.249± 1.757) (P＜0.01),and significantly higher in cases of TNM stages Ⅲ-Ⅳ as compared to those of TNM stages Ⅰ-Ⅱ (P＜0.01).However it was not significantly associated with gender,age or differentiation of cancer (P＞0.05).Conclusions MiR-10a expression is higher in gastric cancer tissues than that in normal gastric tissues,and is associated with lymph node metastases and TNM stage of gastric cancer.MiR-10a may play an important role in the development of gastric cancer and may be a promising target for gastric cancer therapy.
