中国小儿急救医学
中國小兒急救醫學
중국소인급구의학
CHINESE PEDIATRIC EMERGENCY MEDICINE
2013年
3期
265-267
,共3页
马乐%刘正娟%白雪梅%王晴
馬樂%劉正娟%白雪梅%王晴
마악%류정연%백설매%왕청
急性肺损伤%内毒素%水通道蛋白-1%糖皮质激素%大鼠
急性肺損傷%內毒素%水通道蛋白-1%糖皮質激素%大鼠
급성폐손상%내독소%수통도단백-1%당피질격소%대서
Acute lung injury%Lipopolysaccharide%Aquaporin-1%Glucocorticoids%Rat
目的 研究急性肺损伤(acute lung injury,ALI)幼鼠肺组织中水通道蛋白-1(aquaporin1,AQP-1)的表达变化,探讨糖皮质激素治疗ALI的可能机制.方法 将实验动物分为生理盐水对照组(对照组)、致ALI组(lipopolysaccharide,LPS组)、甲泼尼龙(methylprednisolone,Mp)治疗组(Mp组).观察6h后测定动脉血氧分压、肺湿干重比值,并采用免疫组织化学方法和RT-PCR法检测各组大鼠肺组织中AQP-1的表达变化及对肺组织进行常规病理染色观察.结果 RT-PCR结果显示LPS组AQP-1mRNA相对表达量与对照组相比明显减少(0.28±0.05 vs 0.50 ±0.24,P＜0.05),Mp组AQP-1 mRNA相对表达量与LPS组相比明显增加(0.47±0.16 vs 0.28±0.05,P＜0.05).免疫组织化学结果与RT-PCR结果一致.同时Mp组血氧明显改善(P＜0.01),肺湿干重比值下降(P＜0.05),肺2组织损伤减轻.结论 糖皮质激素对ALI的治疗作用可能与其影响AQP-1的表达有关.
目的 研究急性肺損傷(acute lung injury,ALI)幼鼠肺組織中水通道蛋白-1(aquaporin1,AQP-1)的錶達變化,探討糖皮質激素治療ALI的可能機製.方法 將實驗動物分為生理鹽水對照組(對照組)、緻ALI組(lipopolysaccharide,LPS組)、甲潑尼龍(methylprednisolone,Mp)治療組(Mp組).觀察6h後測定動脈血氧分壓、肺濕榦重比值,併採用免疫組織化學方法和RT-PCR法檢測各組大鼠肺組織中AQP-1的錶達變化及對肺組織進行常規病理染色觀察.結果 RT-PCR結果顯示LPS組AQP-1mRNA相對錶達量與對照組相比明顯減少(0.28±0.05 vs 0.50 ±0.24,P＜0.05),Mp組AQP-1 mRNA相對錶達量與LPS組相比明顯增加(0.47±0.16 vs 0.28±0.05,P＜0.05).免疫組織化學結果與RT-PCR結果一緻.同時Mp組血氧明顯改善(P＜0.01),肺濕榦重比值下降(P＜0.05),肺2組織損傷減輕.結論 糖皮質激素對ALI的治療作用可能與其影響AQP-1的錶達有關.
목적 연구급성폐손상(acute lung injury,ALI)유서폐조직중수통도단백-1(aquaporin1,AQP-1)적표체변화,탐토당피질격소치료ALI적가능궤제.방법 장실험동물분위생리염수대조조(대조조)、치ALI조(lipopolysaccharide,LPS조)、갑발니룡(methylprednisolone,Mp)치료조(Mp조).관찰6h후측정동맥혈양분압、폐습간중비치,병채용면역조직화학방법화RT-PCR법검측각조대서폐조직중AQP-1적표체변화급대폐조직진행상규병리염색관찰.결과 RT-PCR결과현시LPS조AQP-1mRNA상대표체량여대조조상비명현감소(0.28±0.05 vs 0.50 ±0.24,P＜0.05),Mp조AQP-1 mRNA상대표체량여LPS조상비명현증가(0.47±0.16 vs 0.28±0.05,P＜0.05).면역조직화학결과여RT-PCR결과일치.동시Mp조혈양명현개선(P＜0.01),폐습간중비치하강(P＜0.05),폐2조직손상감경.결론 당피질격소대ALI적치료작용가능여기영향AQP-1적표체유관.
Objective To explore the mechanism of glucocorticoids in the treatment of acute lung injury(ALI) by studying the expression changes of aquaporin-1 (AQP-1) in the lung.Methods SpragueDawley rats were randomly divided into 3 groups,which were ALI group injected by lipopolysaccharide (LPS group),therapy group treated by methylprednisolone (Mp) after LPS injection (Mp group) and control group injected by normal saline.PaO2 and wet/dry mass ratio were assayed at 6 hours after the injection.Changes of AQP-1 expression in the lungs were analyzed by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR),and pathological examination was performed in each group.Results RT-PCR results showed that the expression of AQP-1 in LPS group was lower than that in control group (0.28 ± 0.05 vs 0.50 ± 0.24,P ＜ 0.05),while the expression of AQP-1 in Mp group was higher than that in LPS group (0.47 ±0.16 vs 0.28 ±0.05,P ＜0.05).The immunohistochemistry results were consistent to the RT-PCR results,meanwhile glucocorticoids therapy could improve the blood oxygen level (P ＜ 0.01),reduce the wet/dry mass ratio (P ＜ 0.05) and relieve the lung injury.Conclusion Glucocorticoids may have beneficial effects on ALI by affecting the expression of AQP-1 in the lungs of rats.
