CAJ | 학술논문

目的探讨脓毒症患儿血小板α颗粒膜糖蛋白(CD62p)、溶酶体膜蛋白(CD63)及中性粒细胞表面膜糖蛋白(CD64)的变化及意义.方法 2010年3月至2013年3月哈尔滨市儿童医院感染内科收治的脓毒症患儿56例,其中严重脓毒症16例,一般脓毒症40例,另设健康对照组34例.应用流式细胞术检测儿童外周血的血小板表面活性标记糖蛋白CD62p、CD63及中性粒细胞CD64表达,并进行比较.结果严重脓毒症组患儿外周血的CD62p、CD63及CD64水平明显高于一般脓毒症组,差异有统计学意义(P＜0.01);一般脓毒症组患儿CD62p、CD63及CD64水平明显高于健康对照组,差异有统计学意义(P＜0.01).相关性分析显示,脓毒症患儿的CD62p、CD63与CD64水平呈正相关(r =0.817、0.796,P均＜0.001),CD62p、CD63、CD64水平与小儿危重病例评分呈正相关(CD62p:r=0.883,P＜0.001;CD63:r=0.862,P＜0.001;CD64:r =0.805.P＜0.001).结论 CD62p、CD63、CD64与感染和炎症严重程度密切相关,可作为评价疾病严重程度及预后的指标.
목적 탐토농독증환인혈소판α과립막당단백(CD62p)、용매체막단백(CD63)급중성립세포표면막당단백(CD64)적변화급의의.방법 2010년3월지2013년3월합이빈시인동의원감염내과수치적농독증환인56례,기중엄중농독증16례,일반농독증40례,령설건강대조조34례.응용류식세포술검측인동외주혈적혈소판표면활성표기당단백CD62p、CD63급중성립세포CD64표체,병진행비교.결과 엄중농독증조환인외주혈적CD62p、CD63급CD64수평명현고우일반농독증조,차이유통계학의의(P＜0.01);일반농독증조환인CD62p、CD63급CD64수평명현고우건강대조조,차이유통계학의의(P＜0.01).상관성분석현시,농독증환인적CD62p、CD63여CD64수평정정상관(r =0.817、0.796,P균＜0.001),CD62p、CD63、CD64수평여소인위중병례평분정정상관(CD62p:r=0.883,P＜0.001;CD63:r=0.862,P＜0.001;CD64:r =0.805.P＜0.001).결론 CD62p、CD63、CD64여감염화염증엄중정도밀절상관,가작위평개질병엄중정도급예후적지표.
Objective To investigate the expression and clinical significance of platelet glycoprotein CD62p,CD63 and neutrophil surface CD64 in sepsis.Methods Fifty-six children with sepsis from March 2010 to March 2013 in Communicable Disease Department of our hospital were divided into severe sepsis group(n =16) and general sepsis group (n =40),normal control group included 34 subjects from health check.CD62p,CD63 and CD64 were detected by flow cytometry in children with sepsis,and compared with normal control group.Results The levels of CD62p,CD63 and CD64 in severe sepsis group were higher than those of general sepsis group (P ＜ 0.01).The levels of CD62p,CD63 and CD64 in general sepsis group were higher than those of normal control group (P ＜ 0.01).Correlation analysis indicated that CD62p and CD63 were in positive correlation with CD64 in children with sepsis(r =0.817,0.796,P ＜0.001).The positive correlations of CD62p,CD63 and CD64 with pediatric critical illness score were also found(CD62p:r =0.883,P ＜0.001;CD63:r=0.862,P ＜0.001;CD64:r=0.805,P ＜0.001).Conclusion CD62p,CD63 and CD64 are closely related to the severity of infection and diseases,and may be used as immune parameters for the estimation of the clinical severity and the prognosis of acute and severe diseases.