CAJ | 학술논문

目的探讨新生鼠坏死性小肠结肠炎(necrotizing enterocolitis,NEC)时肠上皮细胞Bax表达与凋亡相关性.方法出生当日48只清洁级SD新生鼠采用奇、偶数方法随机分为对照组和NEC模型组,每组24只.对照组由母鼠喂养.模型组与母鼠分开,通过代乳品人工喂养+缺氧-复氧-冷刺激建立新生鼠NEC动物模型.分别于实验开始后24h、48 h、72 h留取大鼠回盲部近段回肠组织行免疫组织化学法检查肠上皮细胞Bax表达,原位末端标记法检测肠上皮细胞凋亡率.结果与对照组[(666.55±15.77)IOD; (4.73±0.04)％]相比,NEC模型组新生鼠肠上皮细胞Bax表达和细胞凋亡率在实验开始后24 h开始升高[(1 005.06±11.96) IOD; (15.04 ±0.24)％](P＜0.01),72 h后达到高峰[(3 340.66±68.72) IOD; (35.65±0.61)％](P＜0.01).NEC模型组新生鼠肠上皮细胞凋亡率与肠上皮细胞Bax表达量呈正相关(r=0.94,P＜0.01).结论新生鼠NEC肠上皮细胞凋亡与Bax表达有明确相关性.新生鼠NEC肠上皮细胞可能通过高表达Bax导致肠上皮细胞凋亡.
목적 탐토신생서배사성소장결장염(necrotizing enterocolitis,NEC)시장상피세포Bax표체여조망상관성.방법 출생당일48지청길급SD신생서채용기、우수방법수궤분위대조조화NEC모형조,매조24지.대조조유모서위양.모형조여모서분개,통과대유품인공위양+결양-복양-랭자격건립신생서NEC동물모형.분별우실험개시후24h、48 h、72 h류취대서회맹부근단회장조직행면역조직화학법검사장상피세포Bax표체,원위말단표기법검측장상피세포조망솔.결과 여대조조[(666.55±15.77)IOD; (4.73±0.04)％]상비,NEC모형조신생서장상피세포Bax표체화세포조망솔재실험개시후24 h개시승고[(1 005.06±11.96) IOD; (15.04 ±0.24)％](P＜0.01),72 h후체도고봉[(3 340.66±68.72) IOD; (35.65±0.61)％](P＜0.01).NEC모형조신생서장상피세포조망솔여장상피세포Bax표체량정정상관(r=0.94,P＜0.01).결론 신생서NEC장상피세포조망여Bax표체유명학상관성.신생서NEC장상피세포가능통과고표체Bax도치장상피세포조망.
Objective To investigate the correlation of Bax and apoptosis in enterocytes of neonatal rats with necrotizing enterocolitis (NEC).Methods Forty-eight neonatal rats (1 day) were randomly divided into control group (n =24) and NEC model group (n =24) by use of odd and even.The rats in control group were maternal breast-fed.The rats in NEC model group were separated from their mothers.To be given formula feeding,cold exposure after hypoxic-reoxygenation treatment.The intestinal tissue located at the boundary of ileum and caecum of two groups were gained on the 24 h,48 h and 72 h with which that all rats were sacrificed by cutting neck.Section of intestinal tissue were stained with immunohistochemistry to detect the expression of Bax and were stained with TdT mediated dUTP nick end labeling(TUNEL) to evaluate the apoptosis in each group.Results The integrate optical density (IOD) value of expression of Bax in the NEC model group began to increase on the 24 h [(1 005.06-± 11.96) IOD] and reached the summit on the 72 h [(3 340.66 ±68.72)] compared with the control group[(666.55 ± 15.77)IOD].A few of TUNEL positive cells began to increase with time dependence.A lot of TUNEL positive cells could be found in NEC model group on the 24 h [(15.04 ± 0.24) ％],and the apoptotic index reached the peak on the 72 h [(35.65 ±0.61) ％] compared with the control group[(4.73 ±0.04) ％,P ＜0.01]-There was a significantly positive correlation between the cell apoptosis and the ratio change of Bax in NEC model group (r =0.94,P ＜ 0.01).Conclusion There is a significantly positive correlation between the cell apoptosis and the ratio change of Bax in enterocytes of neonatal rats with NEC.The cell apoptosis in enterocytes of neonatal rats with NEC maybe be induced by Bax.