中国小儿急救医学
中國小兒急救醫學
중국소인급구의학
CHINESE PEDIATRIC EMERGENCY MEDICINE
2014年
11期
689-692
,共4页
梁坤%田玲%吴小磊%黄光举%戴秀华%张慧玉
樑坤%田玲%吳小磊%黃光舉%戴秀華%張慧玉
량곤%전령%오소뢰%황광거%대수화%장혜옥
难治性肺炎支原体肺炎%肺炎支原体%肺泡灌洗液%白细胞介素-6%白细胞介素-10%T细胞亚群
難治性肺炎支原體肺炎%肺炎支原體%肺泡灌洗液%白細胞介素-6%白細胞介素-10%T細胞亞群
난치성폐염지원체폐염%폐염지원체%폐포관세액%백세포개소-6%백세포개소-10%T세포아군
Refractory mycoplasma pneumonia%Mycoplasma%Lavage fluid%interleukin-6%intedeukin-10%T cell subsets
目的 探讨难治性肺炎支原体肺炎患儿肺泡灌洗液(BALF)中白细胞介素(IL)-6、IL-10及T细胞亚群水平变化.方法 选取确诊为难治性肺炎支原体肺炎患儿53例作为观察组,选取同期就诊于我院的支气管异物患儿30例作为对照组.采用双抗体夹心ABC-ELISA法检测BALF中IL-6、IL-10水平及采用流式细胞仪检测T细胞免疫表型CD3+、CD4+和CD8+水平.结果 观察组患儿BALF中IL-6、IL-10水平分别为(63.25±18.61) ng/ml、(31.83±8.33) ng/ml,明显高于对照组的(30.51 ±1.34) ng/ml、(11.01 ±2.91) ng/ml,两组比较差异有统计学意义(P＜0.05);观察组患儿BALF中T细胞亚群CD3+、CD4+、CD8+、CD4 +/CD8+分别为(48.47±2.88)％、(21.16±6.29)％、(23.04±4.63)％、0.94±0.33,明显低于对照组的(64.24±3.06)％、(34.34±7.59)％、(26.71±5.29)％、1.56±0.67,两组比较差异有统计学意义(P＜0.05).结论 难治性肺炎支原体肺炎患儿BALF中IL-6、IL-10水平明显增高及T细胞免疫功能紊乱,提示细胞免疫在难治性肺炎支原体肺炎的发病中起重要作用.
目的 探討難治性肺炎支原體肺炎患兒肺泡灌洗液(BALF)中白細胞介素(IL)-6、IL-10及T細胞亞群水平變化.方法 選取確診為難治性肺炎支原體肺炎患兒53例作為觀察組,選取同期就診于我院的支氣管異物患兒30例作為對照組.採用雙抗體夾心ABC-ELISA法檢測BALF中IL-6、IL-10水平及採用流式細胞儀檢測T細胞免疫錶型CD3+、CD4+和CD8+水平.結果 觀察組患兒BALF中IL-6、IL-10水平分彆為(63.25±18.61) ng/ml、(31.83±8.33) ng/ml,明顯高于對照組的(30.51 ±1.34) ng/ml、(11.01 ±2.91) ng/ml,兩組比較差異有統計學意義(P＜0.05);觀察組患兒BALF中T細胞亞群CD3+、CD4+、CD8+、CD4 +/CD8+分彆為(48.47±2.88)％、(21.16±6.29)％、(23.04±4.63)％、0.94±0.33,明顯低于對照組的(64.24±3.06)％、(34.34±7.59)％、(26.71±5.29)％、1.56±0.67,兩組比較差異有統計學意義(P＜0.05).結論 難治性肺炎支原體肺炎患兒BALF中IL-6、IL-10水平明顯增高及T細胞免疫功能紊亂,提示細胞免疫在難治性肺炎支原體肺炎的髮病中起重要作用.
목적 탐토난치성폐염지원체폐염환인폐포관세액(BALF)중백세포개소(IL)-6、IL-10급T세포아군수평변화.방법 선취학진위난치성폐염지원체폐염환인53례작위관찰조,선취동기취진우아원적지기관이물환인30례작위대조조.채용쌍항체협심ABC-ELISA법검측BALF중IL-6、IL-10수평급채용류식세포의검측T세포면역표형CD3+、CD4+화CD8+수평.결과 관찰조환인BALF중IL-6、IL-10수평분별위(63.25±18.61) ng/ml、(31.83±8.33) ng/ml,명현고우대조조적(30.51 ±1.34) ng/ml、(11.01 ±2.91) ng/ml,량조비교차이유통계학의의(P＜0.05);관찰조환인BALF중T세포아군CD3+、CD4+、CD8+、CD4 +/CD8+분별위(48.47±2.88)％、(21.16±6.29)％、(23.04±4.63)％、0.94±0.33,명현저우대조조적(64.24±3.06)％、(34.34±7.59)％、(26.71±5.29)％、1.56±0.67,량조비교차이유통계학의의(P＜0.05).결론 난치성폐염지원체폐염환인BALF중IL-6、IL-10수평명현증고급T세포면역공능문란,제시세포면역재난치성폐염지원체폐염적발병중기중요작용.
Objective To explore the interleukin (IL)-6,IL-10 and T cell subsets levels in bronchoalveolar lavage fluid(BALF) of children with refractory mycoplasma pneumonia.Methods A total of 53 children with refractory mycoplasma pneumonia were selected as the observation group,30 children with bronchial foreign body in our hospital were chosen as controls during the same period.ABC-double antibody sandwich ELISA method was used to detect IL-6,IL-10 levels and the CD3 +,CD4 + and CD8 + T levels were measured by means of flow cytometry in BALF.Results The IL-6 and IL-10 levels in BALF of children in the observation group were (63.25 ± 18.61) ng/ml,(31.83 ± 8.33) ng/ml respectively,and they were significantly higher than those of the controls[(30.51 ± 1.34) ng/ml,(11.01 ± 2.91) ng/ml] (P ＜ 0.05,respectively).The percentage of CD3 +,CD4 +,CD8 + T cells and the ratio of CD4 +/CD8 + T cells in BALF of the observation group were (48.47 ± 2.88)％,(21.16 ± 6.29)％,(23.04 ± 4.63)％,0.94 ± 0.33,respectively,and they were significantly lower than those of the controls [(64.24 ± 3.06) ％,(34.34 ± 7.59) ％,(26.71 ±5.29)％,1.56-±0.67] (P＜0.05,respectively).Conclusion The IL-6,IL-10 levels in BALF of children with refractory mycoplasma pneumonia significantly increased,suggesting that cell-mediated immunity play an important role in the pathogenesis of refractory mycoplasma pneumonia.
