中国小儿急救医学
中國小兒急救醫學
중국소인급구의학
CHINESE PEDIATRIC EMERGENCY MEDICINE
2014年
11期
697-699
,共3页
全身炎症反应综合征%地塞米松%细胞因子
全身炎癥反應綜閤徵%地塞米鬆%細胞因子
전신염증반응종합정%지새미송%세포인자
Systemic inflammatory response syndrome%Dexamethasone%Cytokine
目的 通过应用不同剂量地塞米松干预新生小鼠全身炎症反应综合征(SIRS),检测血清细胞因子IL-8、IL-4、TNF-α的表达变化,探讨地塞米松在SIRS过程中的作用.方法 50只新生小鼠随机分为5组:空白对照组、盐水对照组和治疗组A、B、C,盐水对照组和治疗组小鼠建立SIRS模型.治疗组A:一次性皮下注射地塞米松2 mg/kg;治疗组B:皮下注射地塞米松1 mg/kg,每12小时1次,总量2 mg/kg;治疗组C:皮下注射地塞米松0.5mg/kg,每12小时1次,总量2mg/kg;盐水对照组:一次性皮下注射同体积生理盐水(0.4 ml/kg).建模72 h后所有50只小鼠取尾静脉血清进行ELISA检测各组小鼠IL-4、IL-8、TNF-αt水平.结果 盐水对照组和治疗组A、B、C小鼠IL-4、IL-8、TNF-α表达均高于空白对照组(P均＜ 0.05);治疗组A、B、C小鼠IL-4、IL-8、TNF-α表达均低于盐水对照组(P均＜0.05);治疗组B、C小鼠TNF-α、IL-8表达均低于治疗组A(P均＜0.05),IL-4水平在治疗组A、B、C间无明显差异(P＞0.05).结论 新生小鼠SIRS时应用地塞米松可使促炎因子IL-8、TNF-α及抑炎因子IL-4表达降低,且小剂量多次给予的效果明显优于大剂量单次给予.
目的 通過應用不同劑量地塞米鬆榦預新生小鼠全身炎癥反應綜閤徵(SIRS),檢測血清細胞因子IL-8、IL-4、TNF-α的錶達變化,探討地塞米鬆在SIRS過程中的作用.方法 50隻新生小鼠隨機分為5組:空白對照組、鹽水對照組和治療組A、B、C,鹽水對照組和治療組小鼠建立SIRS模型.治療組A:一次性皮下註射地塞米鬆2 mg/kg;治療組B:皮下註射地塞米鬆1 mg/kg,每12小時1次,總量2 mg/kg;治療組C:皮下註射地塞米鬆0.5mg/kg,每12小時1次,總量2mg/kg;鹽水對照組:一次性皮下註射同體積生理鹽水(0.4 ml/kg).建模72 h後所有50隻小鼠取尾靜脈血清進行ELISA檢測各組小鼠IL-4、IL-8、TNF-αt水平.結果 鹽水對照組和治療組A、B、C小鼠IL-4、IL-8、TNF-α錶達均高于空白對照組(P均＜ 0.05);治療組A、B、C小鼠IL-4、IL-8、TNF-α錶達均低于鹽水對照組(P均＜0.05);治療組B、C小鼠TNF-α、IL-8錶達均低于治療組A(P均＜0.05),IL-4水平在治療組A、B、C間無明顯差異(P＞0.05).結論 新生小鼠SIRS時應用地塞米鬆可使促炎因子IL-8、TNF-α及抑炎因子IL-4錶達降低,且小劑量多次給予的效果明顯優于大劑量單次給予.
목적 통과응용불동제량지새미송간예신생소서전신염증반응종합정(SIRS),검측혈청세포인자IL-8、IL-4、TNF-α적표체변화,탐토지새미송재SIRS과정중적작용.방법 50지신생소서수궤분위5조:공백대조조、염수대조조화치료조A、B、C,염수대조조화치료조소서건립SIRS모형.치료조A:일차성피하주사지새미송2 mg/kg;치료조B:피하주사지새미송1 mg/kg,매12소시1차,총량2 mg/kg;치료조C:피하주사지새미송0.5mg/kg,매12소시1차,총량2mg/kg;염수대조조:일차성피하주사동체적생리염수(0.4 ml/kg).건모72 h후소유50지소서취미정맥혈청진행ELISA검측각조소서IL-4、IL-8、TNF-αt수평.결과 염수대조조화치료조A、B、C소서IL-4、IL-8、TNF-α표체균고우공백대조조(P균＜ 0.05);치료조A、B、C소서IL-4、IL-8、TNF-α표체균저우염수대조조(P균＜0.05);치료조B、C소서TNF-α、IL-8표체균저우치료조A(P균＜0.05),IL-4수평재치료조A、B、C간무명현차이(P＞0.05).결론 신생소서SIRS시응용지새미송가사촉염인자IL-8、TNF-α급억염인자IL-4표체강저,차소제량다차급여적효과명현우우대제량단차급여.
Objective To observe the expression levels of IL-8,IL-4,TNF-α of neonatal mouse with systemic inflammatory response syndrome(SIRS) treated by different doses of dexamethasone.Methods A total of 50 neonatal mice were randomly divided into five groups:blank control group,saline control group and treatment group A,B,C.The saline control group and treatment groups were established into SIRS models,treatment group A:a single high-dose of dexamethasone (2 mg/kg),subcutaneous injection (SC) ; treatment group B:two doses of dexamethasone (1 mg/kg,q1 2 h,total 2 mg/kg,SC) ; treatment group C:four doses of dexamethasone (0.5 mg/kg,q 1 2 h,total 2 mg/kg,SC) ;saline control group:saline of the same volume (0.4 ml/kg,SC).All mice were detected IL-4,IL-8,TNF-α by ELISA 72 hours after animal models being completed.Results The levels of IL-4,IL-8,TNF-α in saline control group and treatment group A,B,C were higher than those in blank control group respectively (P ＜ 0.05,respectively).The levels of IL-4,IL-8,TNF-α in treatment group A,B,C were lower compared to those of saline control group respectively (P ＜ 0.05,respectively),levels of TNF-α,IL-8 in treatment group B and C were lower than those of treatment group A(P ＜ 0.05,respectively).There were no significant differences in the level of IL-4 among treatment group A,B,and C (P ＞ 0.05).Conclusion Dexamethasone could lower the expressions of pro-inflammatory cytokines IL-8,TNF-α and anti-inflammatory factor IL-8 of neonatal mouse with SIRS,and the effect of multiple low-doses of dexamethasone on SIRS is significantly better than a single high dose.
