CAJ | 학술논문

肝癌是我国常见的恶性肿瘤,针对肝癌的传统治疗方法疗效欠佳、患者预后较差.近10余年来,随着分子生物学技术的发展,肝癌的基因治疗成为该领域新的研究方向和热点,其中以腺病毒为载体的单纯疱疹病毒胸苷激酶自杀基因系统(ADV-tk)对肝癌的治疗研究开展得最早也最为广泛.其原理是把单纯疱疹病毒胸苷激酶基因通过腺病毒导入细胞内,利用其产生的酶将无毒的药物前体更昔洛韦(GCV)转变成细胞毒性产物,从而杀死肝癌细胞.多项动物实验和临床研究结果表明:ADV-tk/GCV系统是治疗肝癌的有效方法.本文总结近年来ADV-tk/GCV系统在肝癌治疗中取得的研究进展,分别从载体的发展过程、基因的作用机制、导入方式、增强杀伤效力、降低肝毒性以及相关动物模型的改进等几个方面进行综述.
간암시아국상견적악성종류,침대간암적전통치료방법료효흠가、환자예후교차.근10여년래,수착분자생물학기술적발전,간암적기인치료성위해영역신적연구방향화열점,기중이선병독위재체적단순포진병독흉감격매자살기인계통(ADV-tk)대간암적치료연구개전득최조야최위엄범.기원리시파단순포진병독흉감격매기인통과선병독도입세포내,이용기산생적매장무독적약물전체경석락위(GCV)전변성세포독성산물,종이살사간암세포.다항동물실험화림상연구결과표명:ADV-tk/GCV계통시치료간암적유효방법.본문총결근년래ADV-tk/GCV계통재간암치료중취득적연구진전,분별종재체적발전과정、기인적작용궤제、도입방식、증강살상효력、강저간독성이급상관동물모형적개진등궤개방면진행종술.
Liver cancer is one of the most common malignant tumors in China.The efficacy of traditional treatment for liver cancer is unsatisfactory,and the prognosis of the patients is poor.In recent 10 years,with the development of the molecular biological techniques,genetic therapy has become a new and promising approach for liver cancer.Of which,adenovirus mediated herpes simplex virus thymidine kinase (ADV-tk) for the treatment of liver cancer is widely applied.The enzyme secreted by ADV-tk transformed the prodrug gancyclovir (GCV) to the cytotoxic agents and thus to kill the liver cancer cells.The results of multiple animal and clinical experiments showed that ADV-tk/GCV is effective for the treatment of liver cancer.In this article,the recent progress of ADV-tk/GCV in the treatment of liver cancer was reviewed.