CAJ | 학술논문

目的观察Ghrelin及受体GHSR蛋白在先天性巨结肠(HD)狭窄段、移行段及扩张段的表达变化及意义.方法 18例先天性巨结肠患儿中男11例,女7例,年龄28 d～6岁.短段型4例,普通型12例,长段型2例.均采用经肛门巨结肠根治术,手术过程中切除的废弃肠段,分出狭窄段,移行段,扩张段.免疫组织化学法测定Ghrelin及受体GHSR蛋白的表达变化.结果 Ghrelin 及其受体GHSR蛋白主要分布于结肠的腺管上皮细胞间及绒毛内.Ghrelin及其受体GHSR蛋白表达上狭窄段最少0.1460±0.014和0.1386±0.016,扩张段最多0.5103±0.013和0.5094±0.019,差异有统计学意义(P＜0.05).同一段组织Ghrelin及其受体GHSR蛋白表达量无差异(P＞0.05).结论 Ghrelin及其受体GHSR蛋白在先天性巨结肠狭窄段的表达减少,可能在胚胎发育过程中影响外胚层细胞发育从而影响结肠神经发育过程,还可能作为一种肠神经递质,导致肠蠕动功能障碍.
목적 관찰Ghrelin급수체GHSR단백재선천성거결장(HD)협착단、이행단급확장단적표체변화급의의.방법 18례선천성거결장환인중남11례,녀7례,년령28 d～6세.단단형4례,보통형12례,장단형2례.균채용경항문거결장근치술,수술과정중절제적폐기장단,분출협착단,이행단,확장단.면역조직화학법측정Ghrelin급수체GHSR단백적표체변화.결과 Ghrelin 급기수체GHSR단백주요분포우결장적선관상피세포간급융모내.Ghrelin급기수체GHSR단백표체상협착단최소0.1460±0.014화0.1386±0.016,확장단최다0.5103±0.013화0.5094±0.019,차이유통계학의의(P＜0.05).동일단조직Ghrelin급기수체GHSR단백표체량무차이(P＞0.05).결론 Ghrelin급기수체GHSR단백재선천성거결장협착단적표체감소,가능재배태발육과정중영향외배층세포발육종이영향결장신경발육과정,환가능작위일충장신경체질,도치장연동공능장애.
Objective To study the expressions of Ghrelin and its receptor (GHSR) in the patients with Hirschsprungs disease (HD).Methods Eighteen patients who were diagnosed with HD and underwent transanal pull-through procedure were recruited in this study.The resected colon was collected to study the expressions of Ghrelin and GHSR by immunohistochemistry staining.Results Ghrelin and GHSR protein were consistently expressed in the glandular epithelium and villi of the ganglionic colon.In the aganglionic segment,the expressions of Ghrelin and GHSR were significantly reduced (Ghrelin:0.1460 ± 0.014,GHSR:0.1386 ± 0.016).However,the expressions of Ghrelin and GHSR were restored in the dilated segment of colon (Ghrelin..0.5103 ± 0.013,GHSR..0.5094 ±0.019).Moderate expressions of Ghrelin and GHSR were found in the transition zone.In the same tissue of colon,the expression of Ghrelin was not significantly different from that of GHSR (P＞0.05).Conclusions The expressions of Ghrelin and GHSR are reduced in the aganglionic colon of the patients with HD.