中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2013年
9期
845-850
,共6页
畅颖%高飞%李彬%张志豹%顼晓琳
暢穎%高飛%李彬%張誌豹%頊曉琳
창영%고비%리빈%장지표%욱효림
肿瘤%视网膜母细胞瘤%异黏蛋白%组织病理学/高危因素%侵袭%转移%免疫组织化学%实时聚合酶链反应
腫瘤%視網膜母細胞瘤%異黏蛋白%組織病理學/高危因素%侵襲%轉移%免疫組織化學%實時聚閤酶鏈反應
종류%시망막모세포류%이점단백%조직병이학/고위인소%침습%전이%면역조직화학%실시취합매련반응
Neoplasm%Retinoblastoma%Metadherin%Histopathology/high-risk factor%Invasion%Metatasis%Immunohistochemistry%Real-time PCR
背景 异黏蛋白(MTDH)基因是近年来发现的一个原癌基因,在恶性实体肿瘤中呈高表达,与恶性肿瘤的浸润、侵袭及转移等生物学行为密切相关.目前,国内外鲜见关于MTDH与视网膜母细胞瘤(RB)的相关研究报道.目的 研究不同侵袭潜能人RB细胞系及RB组织(石蜡切片)中MTDH的表达,探讨其与肿瘤临床分期、病程、细胞分化、组织病理学高危因素之间的关系.方法 应用荧光实时定量PCR(real-time PCR)技术检测RB细胞系Y79、WERI-RB1、SO-RB50中MTDH mRNA的表达水平；采用免疫组织化学Elivision法检测MTDH在54例RB组织中的表达情况,并结合临床组织病理学资料进行统计学分析.结果 MTDH mRNA在Y79、SO-RB50、WERI-RB1细胞系中的相对表达量分别为6.11±±0.17、6.21±0.21及3.97±0.17,3种细胞系中的MTDH mRNA表达差异有统计学意义(F＝ 142.643,P＜0.05),具有潜在侵袭性的Y79细胞系、SO-RB50细胞系中的表达量明显高于WERI-RB1细胞系,差异均有统计学意义(均P=0.000).MTDH在RB组织中主要表达于细胞膜及细胞质,其中35例(占64.81％)标本为MTDH呈强阳性表达,其中临床分期为E期者MTDH表达积分明显高于D期者,有组织病理学高危因素者MTDH表达积分明显高于无高危因素者,侵犯筛板后视神经者MTDH表达积分明显高于未侵犯者,差异均有统计学意义(P=0.035、0.002、0.017).不同年龄(P=0.579)、不同患者性别(P=0.513)、不同眼别(P=0.305)、不同病程(P=0.860)、不同细胞分化类型(P=0.537)、不同脉络膜受累范围(P=0.238)及眼前节是否受累(P=0.579)之间RB组织中MTDH表达积分的差异均无统计学意义.结论 MTDH在RB组织中呈高表达,MTDH基因的激活可能与RB的侵袭转移机制有关,MTDH基因的检测有可能成为判断RB患者预后的重要指标之一.
揹景 異黏蛋白(MTDH)基因是近年來髮現的一箇原癌基因,在噁性實體腫瘤中呈高錶達,與噁性腫瘤的浸潤、侵襲及轉移等生物學行為密切相關.目前,國內外鮮見關于MTDH與視網膜母細胞瘤(RB)的相關研究報道.目的 研究不同侵襲潛能人RB細胞繫及RB組織(石蠟切片)中MTDH的錶達,探討其與腫瘤臨床分期、病程、細胞分化、組織病理學高危因素之間的關繫.方法 應用熒光實時定量PCR(real-time PCR)技術檢測RB細胞繫Y79、WERI-RB1、SO-RB50中MTDH mRNA的錶達水平；採用免疫組織化學Elivision法檢測MTDH在54例RB組織中的錶達情況,併結閤臨床組織病理學資料進行統計學分析.結果 MTDH mRNA在Y79、SO-RB50、WERI-RB1細胞繫中的相對錶達量分彆為6.11±±0.17、6.21±0.21及3.97±0.17,3種細胞繫中的MTDH mRNA錶達差異有統計學意義(F＝ 142.643,P＜0.05),具有潛在侵襲性的Y79細胞繫、SO-RB50細胞繫中的錶達量明顯高于WERI-RB1細胞繫,差異均有統計學意義(均P=0.000).MTDH在RB組織中主要錶達于細胞膜及細胞質,其中35例(佔64.81％)標本為MTDH呈彊暘性錶達,其中臨床分期為E期者MTDH錶達積分明顯高于D期者,有組織病理學高危因素者MTDH錶達積分明顯高于無高危因素者,侵犯篩闆後視神經者MTDH錶達積分明顯高于未侵犯者,差異均有統計學意義(P=0.035、0.002、0.017).不同年齡(P=0.579)、不同患者性彆(P=0.513)、不同眼彆(P=0.305)、不同病程(P=0.860)、不同細胞分化類型(P=0.537)、不同脈絡膜受纍範圍(P=0.238)及眼前節是否受纍(P=0.579)之間RB組織中MTDH錶達積分的差異均無統計學意義.結論 MTDH在RB組織中呈高錶達,MTDH基因的激活可能與RB的侵襲轉移機製有關,MTDH基因的檢測有可能成為判斷RB患者預後的重要指標之一.
배경 이점단백(MTDH)기인시근년래발현적일개원암기인,재악성실체종류중정고표체,여악성종류적침윤、침습급전이등생물학행위밀절상관.목전,국내외선견관우MTDH여시망막모세포류(RB)적상관연구보도.목적 연구불동침습잠능인RB세포계급RB조직(석사절편)중MTDH적표체,탐토기여종류림상분기、병정、세포분화、조직병이학고위인소지간적관계.방법 응용형광실시정량PCR(real-time PCR)기술검측RB세포계Y79、WERI-RB1、SO-RB50중MTDH mRNA적표체수평；채용면역조직화학Elivision법검측MTDH재54례RB조직중적표체정황,병결합림상조직병이학자료진행통계학분석.결과 MTDH mRNA재Y79、SO-RB50、WERI-RB1세포계중적상대표체량분별위6.11±±0.17、6.21±0.21급3.97±0.17,3충세포계중적MTDH mRNA표체차이유통계학의의(F＝ 142.643,P＜0.05),구유잠재침습성적Y79세포계、SO-RB50세포계중적표체량명현고우WERI-RB1세포계,차이균유통계학의의(균P=0.000).MTDH재RB조직중주요표체우세포막급세포질,기중35례(점64.81％)표본위MTDH정강양성표체,기중림상분기위E기자MTDH표체적분명현고우D기자,유조직병이학고위인소자MTDH표체적분명현고우무고위인소자,침범사판후시신경자MTDH표체적분명현고우미침범자,차이균유통계학의의(P=0.035、0.002、0.017).불동년령(P=0.579)、불동환자성별(P=0.513)、불동안별(P=0.305)、불동병정(P=0.860)、불동세포분화류형(P=0.537)、불동맥락막수루범위(P=0.238)급안전절시부수루(P=0.579)지간RB조직중MTDH표체적분적차이균무통계학의의.결론 MTDH재RB조직중정고표체,MTDH기인적격활가능여RB적침습전이궤제유관,MTDH기인적검측유가능성위판단RB환자예후적중요지표지일.
Background Metadherin is a newly discovered oncogene.It is highly expressed in some solid tumors and plays a significant role in invasion and metastasis of neoplasm as an important biological marker of aggressive cancers.However,there is little data available for the relationship between metadherin expression and clinicopathologic features of retinoblastoma(RB).Objective This study was to investigate the expression of the metadherin in RB cell lines and specimens as well as its clinical significance.Methods The expression of metadherin mRNA in different metastatic potential of RB cell lines(Y79,WERI-RB1 and SO-RB50)was detected by real-time PCR,and the protein expression of metadherin (MDTH)in paraffin-embedded RB tissue was detected by immunohistochemistry.The relevance of metadherin expression to clinical histopathological features was statistically analyzed.Results The relative expression value of metadherin mRNA was 6.11±0.17,6.21±0.21 and 3.97±0.17 in Y79,SORB50,WERI-RB1,respectively,with a significant difference among the three types of cell lines(F =142.643,P＜0.05).The relative expression value of metadherin mRNA was significantly higher in Y79 or SO-RB50 than that in W ERI-RB1 (P=0.000).The expression of MTDH protein mostly located in the cellular membrane and cytoplasm.Among the 54 RB sections,35 (64.81％)showed a high intensity in expression of metadherin protein.The total score of metadherin protein expression was higher in the E stage of RB than that of D stage(P=0.035),in the patients with high-risk factor than those without high-risk factor(P=0.002)and the cases with optic nerve invasion compared to non-invasion (P＝0.017).However,no significant differences were found in the expression of metadherin protein between different ages (P =0.579),different genders (P =0.513),bilateral eyes (P =0.305),different disease courses (P=0.860),various types of differentiation (P =0.537),different degree of the ehoroid invasion (P =0.238),and with or without invasion of the anterior ocular segment (P =0.579).Conclusions Metadherin appears to be highly expressed in RB cells.The activation of the MTDH gene is associated with invasion and metastasis of RB.These results imply that the dynamic change of metadherin expression probably is a prognostic indicator of RB.
