中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2014年
1期
32-35
,共4页
周伟%靳春杰%孟兆联%吴建国%颜华
週偉%靳春傑%孟兆聯%吳建國%顏華
주위%근춘걸%맹조련%오건국%안화
糖尿病视网膜病变%荧光素眼底血管造影术%疾病模型%大鼠品系/Brown-Norway大鼠,Sprague-Dawley大鼠%色素
糖尿病視網膜病變%熒光素眼底血管造影術%疾病模型%大鼠品繫/Brown-Norway大鼠,Sprague-Dawley大鼠%色素
당뇨병시망막병변%형광소안저혈관조영술%질병모형%대서품계/Brown-Norway대서,Sprague-Dawley대서%색소
Diabetic retinopathy%Fundus fluorescein angiography%Animal model%Rat strain/ Brown-Norway rat,Sprague-Dawley rat%pigment
背景 链脲佐菌素(STZ)诱导的1型糖尿病大鼠模型是目前公认的经典动物模型,常用于糖尿病视网膜病变(DR)的实验研究,但模型的制作多使用无色素大鼠的视网膜血管消化铺片技术,无法用荧光素眼底血管造影(FFA)进行活体动态观察,且实验中需要的动物数量较多.近年来有色素大鼠已逐渐用于制作DR模型,但采用活体动物FFA检测比较两种模型的研究较少. 目的 比较有色素大鼠和无色素大鼠糖尿病模型FFA和视网膜血管消化铺片的表现,选择适合活体监测视网膜血管改变的糖尿病模型大鼠品系. 方法 选择健康雄性Brown-Norway (BN)大鼠和Sprague-Dawley(SD)大鼠各15只,经尾静脉注射55 mg/kg STZ制作1型糖尿病大鼠模型,注射后每周经鼠尾断端采血检测血糖,血糖值≥16.7 mmol/L为造模成功.造模6周后选取大鼠右眼为实验眼,用裂隙灯显微镜观察模型鼠的眼前节变化,用眼底照相和FFA检查观察大鼠眼底血管变化,然后制备模型鼠离体视网膜血管消化铺片,过碘酸希夫染色后在光学显微镜下观察模型鼠视网膜血管的病理改变,比较2种不同品系大鼠视网膜血管在同样的病理改变下FFA的表现.结果 造模后6周,共纳入造模成功的BN大鼠11只和SD大鼠10只,2种大鼠血糖分别为(24.73±2.98) mmol/L和(22.36±3.65) mmol/L,差异无统计学意义(t=7.873,P＞0.05).BN大鼠组发生晶状体混浊者6眼,SD大鼠组5眼,差异无统计学意义(P=0.717).FFA检查显示,BN大鼠组眼底在棕褐色背景下视网膜血管显影清晰,无脉络膜血管背景荧光干扰,9眼可见血管迂曲、荧光素渗漏等背景期糖尿病视网膜血管改变,而SD大鼠组由于脉络膜背景荧光和涡静脉的干扰,视网膜血管显影不清晰,未见背景期糖尿病视网膜血管改变.视网膜血管消化铺片显示,2种不同品系大鼠均被证实有视网膜毛细血管管径变化、周细胞数目的减少及血管内皮细胞数目的增加,BN大鼠组和SD大鼠组视网膜内皮细胞/周细胞(E/P)分别为11.50±3.68和12.86±3.94,差异无统计学意义(t=9.785,P＞0.05).结论 通过活体FFA观察,BN模型大鼠早期糖尿病视网膜血管改变的成像效果优于SD大鼠.
揹景 鏈脲佐菌素(STZ)誘導的1型糖尿病大鼠模型是目前公認的經典動物模型,常用于糖尿病視網膜病變(DR)的實驗研究,但模型的製作多使用無色素大鼠的視網膜血管消化鋪片技術,無法用熒光素眼底血管造影(FFA)進行活體動態觀察,且實驗中需要的動物數量較多.近年來有色素大鼠已逐漸用于製作DR模型,但採用活體動物FFA檢測比較兩種模型的研究較少. 目的 比較有色素大鼠和無色素大鼠糖尿病模型FFA和視網膜血管消化鋪片的錶現,選擇適閤活體鑑測視網膜血管改變的糖尿病模型大鼠品繫. 方法 選擇健康雄性Brown-Norway (BN)大鼠和Sprague-Dawley(SD)大鼠各15隻,經尾靜脈註射55 mg/kg STZ製作1型糖尿病大鼠模型,註射後每週經鼠尾斷耑採血檢測血糖,血糖值≥16.7 mmol/L為造模成功.造模6週後選取大鼠右眼為實驗眼,用裂隙燈顯微鏡觀察模型鼠的眼前節變化,用眼底照相和FFA檢查觀察大鼠眼底血管變化,然後製備模型鼠離體視網膜血管消化鋪片,過碘痠希伕染色後在光學顯微鏡下觀察模型鼠視網膜血管的病理改變,比較2種不同品繫大鼠視網膜血管在同樣的病理改變下FFA的錶現.結果 造模後6週,共納入造模成功的BN大鼠11隻和SD大鼠10隻,2種大鼠血糖分彆為(24.73±2.98) mmol/L和(22.36±3.65) mmol/L,差異無統計學意義(t=7.873,P＞0.05).BN大鼠組髮生晶狀體混濁者6眼,SD大鼠組5眼,差異無統計學意義(P=0.717).FFA檢查顯示,BN大鼠組眼底在棕褐色揹景下視網膜血管顯影清晰,無脈絡膜血管揹景熒光榦擾,9眼可見血管迂麯、熒光素滲漏等揹景期糖尿病視網膜血管改變,而SD大鼠組由于脈絡膜揹景熒光和渦靜脈的榦擾,視網膜血管顯影不清晰,未見揹景期糖尿病視網膜血管改變.視網膜血管消化鋪片顯示,2種不同品繫大鼠均被證實有視網膜毛細血管管徑變化、週細胞數目的減少及血管內皮細胞數目的增加,BN大鼠組和SD大鼠組視網膜內皮細胞/週細胞(E/P)分彆為11.50±3.68和12.86±3.94,差異無統計學意義(t=9.785,P＞0.05).結論 通過活體FFA觀察,BN模型大鼠早期糖尿病視網膜血管改變的成像效果優于SD大鼠.
배경 련뇨좌균소(STZ)유도적1형당뇨병대서모형시목전공인적경전동물모형,상용우당뇨병시망막병변(DR)적실험연구,단모형적제작다사용무색소대서적시망막혈관소화포편기술,무법용형광소안저혈관조영(FFA)진행활체동태관찰,차실험중수요적동물수량교다.근년래유색소대서이축점용우제작DR모형,단채용활체동물FFA검측비교량충모형적연구교소. 목적 비교유색소대서화무색소대서당뇨병모형FFA화시망막혈관소화포편적표현,선택괄합활체감측시망막혈관개변적당뇨병모형대서품계. 방법 선택건강웅성Brown-Norway (BN)대서화Sprague-Dawley(SD)대서각15지,경미정맥주사55 mg/kg STZ제작1형당뇨병대서모형,주사후매주경서미단단채혈검측혈당,혈당치≥16.7 mmol/L위조모성공.조모6주후선취대서우안위실험안,용렬극등현미경관찰모형서적안전절변화,용안저조상화FFA검사관찰대서안저혈관변화,연후제비모형서리체시망막혈관소화포편,과전산희부염색후재광학현미경하관찰모형서시망막혈관적병리개변,비교2충불동품계대서시망막혈관재동양적병리개변하FFA적표현.결과 조모후6주,공납입조모성공적BN대서11지화SD대서10지,2충대서혈당분별위(24.73±2.98) mmol/L화(22.36±3.65) mmol/L,차이무통계학의의(t=7.873,P＞0.05).BN대서조발생정상체혼탁자6안,SD대서조5안,차이무통계학의의(P=0.717).FFA검사현시,BN대서조안저재종갈색배경하시망막혈관현영청석,무맥락막혈관배경형광간우,9안가견혈관우곡、형광소삼루등배경기당뇨병시망막혈관개변,이SD대서조유우맥락막배경형광화와정맥적간우,시망막혈관현영불청석,미견배경기당뇨병시망막혈관개변.시망막혈관소화포편현시,2충불동품계대서균피증실유시망막모세혈관관경변화、주세포수목적감소급혈관내피세포수목적증가,BN대서조화SD대서조시망막내피세포/주세포(E/P)분별위11.50±3.68화12.86±3.94,차이무통계학의의(t=9.785,P＞0.05).결론 통과활체FFA관찰,BN모형대서조기당뇨병시망막혈관개변적성상효과우우SD대서.
Background Streptozotocin (STZ)-induced type 1 diabetic model is an acceptable model and is often used to the study of diabetic retinopathy (DR).However,the model is often established using retinal digest preparation in vitro in albino rats,and therefore it is difficult to evaluate the change of retinal vessels by fundus fluorescein angiography (FFA) in vivo.Recently,pigmented rats are being used as the DR model.But the study on the comparison between albino rat model and pigmented rat model is seldom.Objective This study was to observe and compare the manifestations of FFA and retinal digest preparation in early diabetic pigmented diabetic rat and albino diabetic rat,and to select the right model of DR using FFA.Methods Type 1 diabetic models were induced in 15 six-week-old health male BN rats and 15 six-week-old health male SD rats by the injection of STZ (55 mg/kg) via tail vein.The models were thought to be successful in the rats with the blood glucose level ≥ 16.7 mmol/L.The right eyes of the rats were extracted 6 weeks after injection of STZ.Lens were examined by slit lamp microscope.Retinal vascular changes were examined by fundus photography,FFA and periodic acid Schiff staining of retinal digest preparation.The manifestations of FFA and retinal digest preparation were contrasted between BN rats and SD rats.The number of eyes with lens opacification was compared by Chi-square test and the ratio of vascular endothelial cells and perithelial cells (E/P) was compared between BN rats and SD rats.The use and care of experimental animals complied the Statement of Ethic Committee of Tianjin Medicine University.Results Six weeks after injection of STZ,11 BN rats and 10SD rats were included in this study.The blood levels were (24.73±2.98) mmol/L and (22.36±3.65) mmol/L in BN rats and SD rats,respectively,without significant difference between the 2 types of rats (t =7.873,P＞0.05).Lens opacification occurred in 6 BN rats and in 5 SD rats (P=0.717).FFA showed the clear retinal vascular under the brown background.Evident vessel disorder and fluorescence leakage like background stage of DR were seen in 9 eyes.However,in the SD rats,retinal vessel abnormality could not exhibited owing to the interference of choroid fluorescent light from choroidal vessels and vortex vein.Retinal digest preparation exhibited that unevenness of vessel diameter,decrease of perithelial cells and increase of endothelial cells in both types of rats.The E/P values were 11.50±3.68in the BN rats and 12.86±3.94 in the SD rats,without significant difference between them (t=9.785,P＞0.05).Conclusions The abnormality of fundus vascular morphology can be better displayed in pigmented diabetic rats than albino rats by FFA in vivo.
