中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2014年
1期
46-50
,共5页
施宇华%丁颖%黄振平%尹莉莉%吴星伟
施宇華%丁穎%黃振平%尹莉莉%吳星偉
시우화%정영%황진평%윤리리%오성위
视网膜%缺血-再灌注损伤%缺血后适应%视网膜电图%视网膜厚度
視網膜%缺血-再灌註損傷%缺血後適應%視網膜電圖%視網膜厚度
시망막%결혈-재관주손상%결혈후괄응%시망막전도%시망막후도
Retina%Ischemia reperfusion injury%Ischemic postconditioning%Electroretinogram%Retinal thickness
背景 研究证明,缺血后适应(IPC)对多种组织器官的缺血缺氧损伤均有一定的抵抗作用,但其对视网膜缺血缺氧的作用仍受到关注. 目的 探讨IPC对大鼠视网膜缺血-再灌注损伤(RIRI)后视网膜结构和功能的保护作用.方法 将36只健康雄性Wistar大鼠以随机数字表法分为正常对照组、伪手术组、缺血-再灌注组、IPC组.利用前房灌注生理盐水升高眼压至100 mmHg(1 mmHg=0.133 kPa)维持60 min的方法制备RIRI大鼠模型,实施IPC处理鼠亚分为再灌注后即刻、1 min、10 min组(即IPC Ⅰ组、IPCⅡ组、IPCⅢ组),分别于实验后1d、7d行大鼠视网膜电图(ERG)检测,然后用过量麻醉法处死大鼠并制备视网膜切片,行苏木精-伊红染色,对各组大鼠视网膜厚度的变化和视网膜形态进行观察.采用SPSS 13.0统计学软件的单因素方差分析对各组大鼠ERG各波振幅恢复率和视网膜厚度值的差异进行比较. 结果 实验后1d,与正常对照组大鼠比较,伪手术组大鼠视网膜结构接近正常,而缺血-再灌注组及IPC Ⅰ组、IPCⅡ组、IPCⅢ组大鼠视网膜均出现水肿,可见空泡变性,主要在内丛状层(IPL)及内核层(INL).缺血-再灌注组及IPC Ⅰ组、IPCⅡ组、IPCⅢ组大鼠视网膜全层、INL、IPL及视网膜外层厚度值均明显高于正常对照组,差异均有统计学意义(均P＜0.05).再灌注后7d,缺血-再灌注组大鼠视网膜全层厚度值明显低于正常对照组,差异均有统计学意义(均P＜0.05),尤以1NL、IPL显著.IPC Ⅰ组、IPCⅡ组、IPCⅢ组大鼠视网膜全层、INL、IPL及视网膜外层厚度值均明显高于缺血-再灌注组,差异均有统计学意义(均P＜0.05).再灌注后7d,缺血-再灌注组、IPC各组大鼠ERGa波、b波和OPs振幅恢复率明显低于伪手术组和正常对照组大鼠,差异均有统计学意义(均P＜0.05);而IPC Ⅰ组、IPCⅡ组、IPCⅢ组大鼠ERG a波、b波和OPs振幅恢复率明显高于缺血-再灌注组,差异均有统计学意义(均P＜0.05).结论 I PC对RIRI具有保护作用,在大鼠模型中,这种保护作用在再灌注后即刻至1 min时最强.
揹景 研究證明,缺血後適應(IPC)對多種組織器官的缺血缺氧損傷均有一定的牴抗作用,但其對視網膜缺血缺氧的作用仍受到關註. 目的 探討IPC對大鼠視網膜缺血-再灌註損傷(RIRI)後視網膜結構和功能的保護作用.方法 將36隻健康雄性Wistar大鼠以隨機數字錶法分為正常對照組、偽手術組、缺血-再灌註組、IPC組.利用前房灌註生理鹽水升高眼壓至100 mmHg(1 mmHg=0.133 kPa)維持60 min的方法製備RIRI大鼠模型,實施IPC處理鼠亞分為再灌註後即刻、1 min、10 min組(即IPC Ⅰ組、IPCⅡ組、IPCⅢ組),分彆于實驗後1d、7d行大鼠視網膜電圖(ERG)檢測,然後用過量痳醉法處死大鼠併製備視網膜切片,行囌木精-伊紅染色,對各組大鼠視網膜厚度的變化和視網膜形態進行觀察.採用SPSS 13.0統計學軟件的單因素方差分析對各組大鼠ERG各波振幅恢複率和視網膜厚度值的差異進行比較. 結果 實驗後1d,與正常對照組大鼠比較,偽手術組大鼠視網膜結構接近正常,而缺血-再灌註組及IPC Ⅰ組、IPCⅡ組、IPCⅢ組大鼠視網膜均齣現水腫,可見空泡變性,主要在內叢狀層(IPL)及內覈層(INL).缺血-再灌註組及IPC Ⅰ組、IPCⅡ組、IPCⅢ組大鼠視網膜全層、INL、IPL及視網膜外層厚度值均明顯高于正常對照組,差異均有統計學意義(均P＜0.05).再灌註後7d,缺血-再灌註組大鼠視網膜全層厚度值明顯低于正常對照組,差異均有統計學意義(均P＜0.05),尤以1NL、IPL顯著.IPC Ⅰ組、IPCⅡ組、IPCⅢ組大鼠視網膜全層、INL、IPL及視網膜外層厚度值均明顯高于缺血-再灌註組,差異均有統計學意義(均P＜0.05).再灌註後7d,缺血-再灌註組、IPC各組大鼠ERGa波、b波和OPs振幅恢複率明顯低于偽手術組和正常對照組大鼠,差異均有統計學意義(均P＜0.05);而IPC Ⅰ組、IPCⅡ組、IPCⅢ組大鼠ERG a波、b波和OPs振幅恢複率明顯高于缺血-再灌註組,差異均有統計學意義(均P＜0.05).結論 I PC對RIRI具有保護作用,在大鼠模型中,這種保護作用在再灌註後即刻至1 min時最彊.
배경 연구증명,결혈후괄응(IPC)대다충조직기관적결혈결양손상균유일정적저항작용,단기대시망막결혈결양적작용잉수도관주. 목적 탐토IPC대대서시망막결혈-재관주손상(RIRI)후시망막결구화공능적보호작용.방법 장36지건강웅성Wistar대서이수궤수자표법분위정상대조조、위수술조、결혈-재관주조、IPC조.이용전방관주생리염수승고안압지100 mmHg(1 mmHg=0.133 kPa)유지60 min적방법제비RIRI대서모형,실시IPC처리서아분위재관주후즉각、1 min、10 min조(즉IPC Ⅰ조、IPCⅡ조、IPCⅢ조),분별우실험후1d、7d행대서시망막전도(ERG)검측,연후용과량마취법처사대서병제비시망막절편,행소목정-이홍염색,대각조대서시망막후도적변화화시망막형태진행관찰.채용SPSS 13.0통계학연건적단인소방차분석대각조대서ERG각파진폭회복솔화시망막후도치적차이진행비교. 결과 실험후1d,여정상대조조대서비교,위수술조대서시망막결구접근정상,이결혈-재관주조급IPC Ⅰ조、IPCⅡ조、IPCⅢ조대서시망막균출현수종,가견공포변성,주요재내총상층(IPL)급내핵층(INL).결혈-재관주조급IPC Ⅰ조、IPCⅡ조、IPCⅢ조대서시망막전층、INL、IPL급시망막외층후도치균명현고우정상대조조,차이균유통계학의의(균P＜0.05).재관주후7d,결혈-재관주조대서시망막전층후도치명현저우정상대조조,차이균유통계학의의(균P＜0.05),우이1NL、IPL현저.IPC Ⅰ조、IPCⅡ조、IPCⅢ조대서시망막전층、INL、IPL급시망막외층후도치균명현고우결혈-재관주조,차이균유통계학의의(균P＜0.05).재관주후7d,결혈-재관주조、IPC각조대서ERGa파、b파화OPs진폭회복솔명현저우위수술조화정상대조조대서,차이균유통계학의의(균P＜0.05);이IPC Ⅰ조、IPCⅡ조、IPCⅢ조대서ERG a파、b파화OPs진폭회복솔명현고우결혈-재관주조,차이균유통계학의의(균P＜0.05).결론 I PC대RIRI구유보호작용,재대서모형중,저충보호작용재재관주후즉각지1 min시최강.
Background The potential protective effect of ischemia postconditiomng for hypoxia has been determined in many tissues and organs.But its mechanism in retina is still lack.Objective The aim of this study was to investigate the protective effect of ischemia postconditioning on retinal ischemia reperfusion injury.Methods Thirty-six health male Wistar rats were randomized into the normal control group,sham group,ischemia reperfusion group and ischemic postconditioning group.Retinal ischemia reperfusion models were induced by elevating the intraocular pressure to 110 mmHg for 60 minutes,and then the ischemic postconditioning was performed right away,1 minute and 10 minutes after ischemia in the rats of the ischemic postconditioning Ⅰ,Ⅱ,Ⅲ group,respectively.Electroretinogram (ERG) was recorded in the rats 7 days after operation,and the rats were sacrificed by excessive anesthesia.Retinal specimens were prepared for the histopathological examination.The retinal thickness and the recovery rates of ERG a,b waves and oscillation potentials (OPs) were compared among the groups using ANOVA of SPSS software.Results One day after experiment,the structure of rat retinas was nearly normal.However,retinal edema and vacuolar degeneration were seen in the rats of the ischemia reperfusion group and ischemic postconditioning Ⅰ,Ⅱ,Ⅲ groups primarily in the inner plexiform layer (IPL),inner nuclear layer (INL).The retinal thickness values of the entire layer,IPL and INL were significantly higher in the ischemia reperfusion group and ischemic postconditioning Ⅰ,Ⅱ,Ⅲ groups compared with the normal control group (all at P＜0.05).Seven days after experiment,the retinas were thinner in the rats of the ischemia reperfusion group than those of the rats in the sham group,but the retinal thickness values in the ischemic postconditioning Ⅰ,Ⅱ,Ⅲ groups were significantly increased in comparison with the ischemia reperfusion group (all at P＜0.05).In addition,the recovery rates of ERG a,b,OPs amplitudes were significantly lower in the ischemia reperfusion group and ischemic postconditioning Ⅰ,Ⅱ,Ⅲ groups compared with the normal control group (all at P＜0.05).However,the recovery rates of ERG a,b,OPs amplitudes evidently elevated in the ischemic postconditioning Ⅰ,Ⅱ,Ⅲ groups in comparison with the ischemia reperfusion group (all at P＜0.05).Conclusions Ischemia postconditioning can protect the structure and function of retina from ischemia reperfusion injury.Ischemia postconditioning plays the maximal effect immediately to 1 minute after ischemia reperfusion injury in Wistar rat.
