中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2014年
5期
388-391
,共4页
苏庆%韩松%郗平%李俊发%傅涛
囌慶%韓鬆%郗平%李俊髮%傅濤
소경%한송%치평%리준발%부도
视皮层%Synapsin%神经元可塑性%磷酸化水平
視皮層%Synapsin%神經元可塑性%燐痠化水平
시피층%Synapsin%신경원가소성%린산화수평
Visual cortex%Synapsin%Neural plasticity%Phosphorylation
背景 视觉发育可塑性与弱视治疗时间和方法的选择密切相关,synapsin(突触素)作为一种突触前特异性蛋白家族,在视觉发育可塑性中的作用尚未明确. 目的 动态观察正常小鼠视皮层内synapsin蛋白表达水平(T-synapsin)及其磷酸化水平(p-synapsin)随生长和发育发生的量的变化.方法 选用清洁级健康新生C57BL/6小鼠42只,分别于出生后第7、14、21、28、35、42、60天取左右两侧视皮层,每个时间点选6只小鼠.采用Western bolt法检测不同鼠龄小鼠视皮层内不同亚型synapsin的表达量及其位点1的磷酸化水平. 结果 正常C57BL/6小鼠视皮层中synapsin的表达随生长和发育发生动态变化,出生后第7、14、21、28、35、42天,小鼠视皮层中T-synapsin Ⅰ a/b的表达量分别为成年小鼠(出生后第60天,P60)表达量的(21.32±3.27)％、(56.27±10.18)％、(77.05±10.05)％、(83.75± 10.52)％、(94.69±11.46)％和(98.75±5.86)％,不同鼠龄小鼠视皮层中T-synapsin Ⅰ a/b表达量的总体比较差异有统计学意义(F=69.538,P＜0.001),其中P7、P14、P21、P28组表达量明显低于成年组,差异均有统计学意义(P＜0.05),P35、P42组与成年组相比差异均无统计学意义(P=0.280、0 798);不同亚型T-synapsin在视皮层中的表达趋势随小鼠的生长和发育略有不同,其中T-synapsinⅡa、Ⅲa增长相对缓慢,P60时仍呈增长趋势,不同鼠龄间T-synapsinⅡa、Ⅱb、Ⅲa表达的总体比较差异均有统计学意义(F=42.492、55.595、39.172,P＜0.001);p-Synapsin Ⅰ a/b的表达在出生后随小鼠的发育而逐渐增高,P21左右达高峰,为成年小鼠磷酸化水平的(2.86±0.17)倍,此后迅速下降,成年后维持低磷酸化水平,不同鼠龄间小鼠视皮层中p-synapsin Ⅰ a/b表达量的总体比较差异有统计学意义(F=22.620,P＜0.001).结论 小鼠视皮层中synapsin的表达量随生长和发育发生的动态变化与视觉发育关键期的起始和终止在时间上具有一定的同步性,synapsin可能参与视觉发育敏感期视皮层神经元可塑性的调节.
揹景 視覺髮育可塑性與弱視治療時間和方法的選擇密切相關,synapsin(突觸素)作為一種突觸前特異性蛋白傢族,在視覺髮育可塑性中的作用尚未明確. 目的 動態觀察正常小鼠視皮層內synapsin蛋白錶達水平(T-synapsin)及其燐痠化水平(p-synapsin)隨生長和髮育髮生的量的變化.方法 選用清潔級健康新生C57BL/6小鼠42隻,分彆于齣生後第7、14、21、28、35、42、60天取左右兩側視皮層,每箇時間點選6隻小鼠.採用Western bolt法檢測不同鼠齡小鼠視皮層內不同亞型synapsin的錶達量及其位點1的燐痠化水平. 結果 正常C57BL/6小鼠視皮層中synapsin的錶達隨生長和髮育髮生動態變化,齣生後第7、14、21、28、35、42天,小鼠視皮層中T-synapsin Ⅰ a/b的錶達量分彆為成年小鼠(齣生後第60天,P60)錶達量的(21.32±3.27)％、(56.27±10.18)％、(77.05±10.05)％、(83.75± 10.52)％、(94.69±11.46)％和(98.75±5.86)％,不同鼠齡小鼠視皮層中T-synapsin Ⅰ a/b錶達量的總體比較差異有統計學意義(F=69.538,P＜0.001),其中P7、P14、P21、P28組錶達量明顯低于成年組,差異均有統計學意義(P＜0.05),P35、P42組與成年組相比差異均無統計學意義(P=0.280、0 798);不同亞型T-synapsin在視皮層中的錶達趨勢隨小鼠的生長和髮育略有不同,其中T-synapsinⅡa、Ⅲa增長相對緩慢,P60時仍呈增長趨勢,不同鼠齡間T-synapsinⅡa、Ⅱb、Ⅲa錶達的總體比較差異均有統計學意義(F=42.492、55.595、39.172,P＜0.001);p-Synapsin Ⅰ a/b的錶達在齣生後隨小鼠的髮育而逐漸增高,P21左右達高峰,為成年小鼠燐痠化水平的(2.86±0.17)倍,此後迅速下降,成年後維持低燐痠化水平,不同鼠齡間小鼠視皮層中p-synapsin Ⅰ a/b錶達量的總體比較差異有統計學意義(F=22.620,P＜0.001).結論 小鼠視皮層中synapsin的錶達量隨生長和髮育髮生的動態變化與視覺髮育關鍵期的起始和終止在時間上具有一定的同步性,synapsin可能參與視覺髮育敏感期視皮層神經元可塑性的調節.
배경 시각발육가소성여약시치료시간화방법적선택밀절상관,synapsin(돌촉소)작위일충돌촉전특이성단백가족,재시각발육가소성중적작용상미명학. 목적 동태관찰정상소서시피층내synapsin단백표체수평(T-synapsin)급기린산화수평(p-synapsin)수생장화발육발생적량적변화.방법 선용청길급건강신생C57BL/6소서42지,분별우출생후제7、14、21、28、35、42、60천취좌우량측시피층,매개시간점선6지소서.채용Western bolt법검측불동서령소서시피층내불동아형synapsin적표체량급기위점1적린산화수평. 결과 정상C57BL/6소서시피층중synapsin적표체수생장화발육발생동태변화,출생후제7、14、21、28、35、42천,소서시피층중T-synapsin Ⅰ a/b적표체량분별위성년소서(출생후제60천,P60)표체량적(21.32±3.27)％、(56.27±10.18)％、(77.05±10.05)％、(83.75± 10.52)％、(94.69±11.46)％화(98.75±5.86)％,불동서령소서시피층중T-synapsin Ⅰ a/b표체량적총체비교차이유통계학의의(F=69.538,P＜0.001),기중P7、P14、P21、P28조표체량명현저우성년조,차이균유통계학의의(P＜0.05),P35、P42조여성년조상비차이균무통계학의의(P=0.280、0 798);불동아형T-synapsin재시피층중적표체추세수소서적생장화발육략유불동,기중T-synapsinⅡa、Ⅲa증장상대완만,P60시잉정증장추세,불동서령간T-synapsinⅡa、Ⅱb、Ⅲa표체적총체비교차이균유통계학의의(F=42.492、55.595、39.172,P＜0.001);p-Synapsin Ⅰ a/b적표체재출생후수소서적발육이축점증고,P21좌우체고봉,위성년소서린산화수평적(2.86±0.17)배,차후신속하강,성년후유지저린산화수평,불동서령간소서시피층중p-synapsin Ⅰ a/b표체량적총체비교차이유통계학의의(F=22.620,P＜0.001).결론 소서시피층중synapsin적표체량수생장화발육발생적동태변화여시각발육관건기적기시화종지재시간상구유일정적동보성,synapsin가능삼여시각발육민감기시피층신경원가소성적조절.
Background The treatment timing and method of amblyopia rely on the plasticity of visual system.Synapsin is a family of presynaptic terminal specific protein.Its role in visual developmental plasticity is below understood.Objective To investigate the dynamic expressions of synapsin (T-synapsin),and phosphorylation of synapsin (p-synapsin Ⅰ a/b) in visual cortex of normal mice and further explore the role of synapsin in plasticity of visual system.Methods Forty-two clean neonatal C57BL/6 mice were collected.The mice were sacrificed at postnatal 7,14,21,28,35,42,60 days respectively to obtain the tissue samples of visual cortex.Expression levels of T-synapsin and p-synapsin in the visual cortex following the ageing were quantitatively detected using Western blot assay.Results The expression of synapsin in normal mice showed a dynamic increase with the ageing.The T-synapsin Ⅰ a/b/β-actin value in visual cortex was (21.32 ± 3.27) ％,(56.27 ± 10.18) ％,(77.05 ± 10.05) ％,(83.75±10.52) ％,(94.69±11.46)％,(98.75±5.86) ％ of adults mice (postnatal 60 days,P60) in the mice of postnatal 7,14,21,28,35,42 days,respectively,showing a significant difference among them (F =69.538,P ＜ 0.001).Compared with the adult mice,the T-synapsin Ⅰ a/b/β-actin value in the mice of P7,P14,P21,P28 was significantly lower (all at P＜0.05),but no significant difference was found between P35 and P60,P42 and P60 (P =0.280,0.798).The development trend of different synapsin subtypes,such as T-synapsin Ⅰ a/b,T-synapsin Ⅱ a,T-synapsin Ⅱ b and T-synapsin Ⅲ a,was not quite the same during the ageing.The expression of T-synapsin Ⅱ a and Ⅲ a increasing more slowly with development,and kept increasing until P60.Significant differences were found among various age of mice in T-synapsin Ⅱ a,Ⅱ b,Ⅲa respectively(F =42.492 55.595,39.172,all at P＜0.001).The p-synapsin Ⅰ a/b level in the visual cortex elevated with the ageing of the mice,and that peaked in P21 mice,which was (2.86±0.17) times more than that in adult mice.After that,the expression level of p-synapsin Ⅰ a/b dropped rapidly.A significant difference was found in the p-synapsin Ⅰ a/b expression among different ages of mice (F =22.620,P ＜ 0.001).Conclusions Synapsin level in visual cortex presents a developmental change which correlated with the onset and decline of the critical period.Synapsin is probably involved in the regulation of neural plasticity in visual cortex in critical period.
