中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2014年
10期
902-905
,共4页
弥漫大B细胞淋巴瘤/治疗%细胞培养%放射疗法%X射线%细胞周期%凋亡
瀰漫大B細胞淋巴瘤/治療%細胞培養%放射療法%X射線%細胞週期%凋亡
미만대B세포림파류/치료%세포배양%방사요법%X사선%세포주기%조망
Diffuse large B-cell lymphoma/treatment%Cell culture%Radiotherapy%X-ray%Cell cycle%Apoptosis
背景 弥漫大B细胞淋巴瘤(DLBCL)恶性程度高,对放射线敏感,但临床上放射疗法方法各异,合理、有效的放射疗法方案就显得尤其重要. 目的 探讨X射线对DLBCL细胞周期和细胞凋亡的影响,为其临床治疗提供理论依据.方法将人全身DLBCL细胞系进行常规复苏和培养,将细胞悬液接种后分别暴露于2、4、6、8 Gy4种不同剂量的X射线下6、12、24、48 h,采用流式细胞仪分析其细胞周期和细胞凋亡的变化,未接受X射线照射的细胞作为空白对照组.结果 DLBCL细胞复苏后生长活跃,细胞呈圆形或椭圆形,传2～3代细胞生长状态稳定.随着照射剂量的增加和照射时间的延长,G0-G1期细胞比例逐渐降低,总体差异均有统计学意义(F剂量 =45.58,P=0.00;F时间=43.11,P=0.00);而G2-M期细胞比例逐渐增加,差异均有统计学意义(F剂量=40.77,P=0.00;F时间=91.91,P=0.00);细胞凋亡指数逐渐升高,差异均有统计学意义(F剂量=87.36,P=0.00;F时间=37.31,P=0.00). 结论 X射线照射将DLBCL细胞阻滞在G2-M期并导致细胞凋亡,其作用呈剂量和时间依赖性.
揹景 瀰漫大B細胞淋巴瘤(DLBCL)噁性程度高,對放射線敏感,但臨床上放射療法方法各異,閤理、有效的放射療法方案就顯得尤其重要. 目的 探討X射線對DLBCL細胞週期和細胞凋亡的影響,為其臨床治療提供理論依據.方法將人全身DLBCL細胞繫進行常規複囌和培養,將細胞懸液接種後分彆暴露于2、4、6、8 Gy4種不同劑量的X射線下6、12、24、48 h,採用流式細胞儀分析其細胞週期和細胞凋亡的變化,未接受X射線照射的細胞作為空白對照組.結果 DLBCL細胞複囌後生長活躍,細胞呈圓形或橢圓形,傳2～3代細胞生長狀態穩定.隨著照射劑量的增加和照射時間的延長,G0-G1期細胞比例逐漸降低,總體差異均有統計學意義(F劑量 =45.58,P=0.00;F時間=43.11,P=0.00);而G2-M期細胞比例逐漸增加,差異均有統計學意義(F劑量=40.77,P=0.00;F時間=91.91,P=0.00);細胞凋亡指數逐漸升高,差異均有統計學意義(F劑量=87.36,P=0.00;F時間=37.31,P=0.00). 結論 X射線照射將DLBCL細胞阻滯在G2-M期併導緻細胞凋亡,其作用呈劑量和時間依賴性.
배경 미만대B세포림파류(DLBCL)악성정도고,대방사선민감,단림상상방사요법방법각이,합리、유효적방사요법방안취현득우기중요. 목적 탐토X사선대DLBCL세포주기화세포조망적영향,위기림상치료제공이론의거.방법장인전신DLBCL세포계진행상규복소화배양,장세포현액접충후분별폭로우2、4、6、8 Gy4충불동제량적X사선하6、12、24、48 h,채용류식세포의분석기세포주기화세포조망적변화,미접수X사선조사적세포작위공백대조조.결과 DLBCL세포복소후생장활약,세포정원형혹타원형,전2～3대세포생장상태은정.수착조사제량적증가화조사시간적연장,G0-G1기세포비례축점강저,총체차이균유통계학의의(F제량 =45.58,P=0.00;F시간=43.11,P=0.00);이G2-M기세포비례축점증가,차이균유통계학의의(F제량=40.77,P=0.00;F시간=91.91,P=0.00);세포조망지수축점승고,차이균유통계학의의(F제량=87.36,P=0.00;F시간=37.31,P=0.00). 결론 X사선조사장DLBCL세포조체재G2-M기병도치세포조망,기작용정제량화시간의뢰성.
Background The malignant degree of the diffuse large B-cell lymphoma (DLBCL) is high.Radiation therapy is sensitive,but the therapeutic schedule can not be unified,reasonable and effective therapeutic schedule is important in clinic treatment of the DLBCL.Objective This study was to access the effect of X-ray on the cell cycle and cell apoptosis of the DLBCL.Methods DLBCL cell line was cultured and the cell suspension was inoculated to 12-well cultured plate.The cells exposed to X-ray for 6,12,24,48 hours with the irradiation doses of 2,4,6,8 Gy respectively.The cell proportions of different cycles were assayed by flow cytometer,and cell apoptosis index was evaluated and calculated.Non-irradiation cells served as controls.Results The cells grew well with a good cell vitality and round-like shape,and the growth was stable in generation 2 or 3.With the increase of X-ray doses and the lapse of irradiation time,the cell proportions in G0-G1 phase gradually reduced (Fdose =45.58,P=0.00; Ftime =43.11,P=0.00).However,cell proportions in G2-M phase were gradually increased as the increase of X-ray doses and lapse of irradiation time (Fdose =40.77,P =0.00 ; Ftime =91.91,P =0.00).In addition,the apoptotic proportion of the cells was significantly elevated with the increase of X-ray dose and irradiation time (Fdose =87.36,P =0.00; Ftime =37.31,P =0.00).Conclusions X-ray irradiation retards DLBCL in the G2-M phase and causes cell apoptosis in dose-and time-dependent manner.
