中华眼视光学与视觉科学杂志
中華眼視光學與視覺科學雜誌
중화안시광학여시각과학잡지
CHINESE JOURNAL OF OPTOMETRY OPHTHALMOLOGY AND VISUAL SCIENCE
2013年
8期
484-487
,共4页
胡风云%封利霞%任真%谭佳博
鬍風雲%封利霞%任真%譚佳博
호풍운%봉리하%임진%담가박
酒精中毒%视网膜电描记术%诱发电位,视觉%抗氧化剂
酒精中毒%視網膜電描記術%誘髮電位,視覺%抗氧化劑
주정중독%시망막전묘기술%유발전위,시각%항양화제
Alcoholism%Electroretinography%Evoked potential,visual%Antioxidants
目的 探讨慢性酒精中毒对大鼠视功能的损害以及抗氧化剂ebselen对视功能的保护作用.方法 实验研究.利用自由饮方法建立慢性酒精中毒大鼠模型,按随机数字表将60只大鼠随机分为对照组、酒精组和ebselen治疗组(即药物组),采用闪光视网膜电图(FERG)及闪光视觉诱发电位(FVEP)观察大鼠视功能的改变.对数据进行单因素方差分析.结果 3组FERG a、b波潜时差异有统计学意义(F=4.161、3.664,P＜0.05),酒精组a、b波潜时较对照组明显长(P＜0.05),而药物组与对照组相比差异无统计学意义(P＞0.05).FERG b波振幅酒精组及药物组均较对照组低(F=3.312,P＜0.05).3组FVEP N1波、P1波潜时差异有统计学意义(F=7.940、5.07,P＜0.05),与对照组相比,酒精组N1波、P1波潜时明显较长(P＜0.05).3组FVEP N1-P1波振幅差异有统计学意义(F=3.874,P＜0.05),与对照组相比,N1-P1波振幅明显较低(P＜0.05).药物组N1、P1波潜时及N1-P1振幅与对照组相比差异均无统计学意义(P＞0.05).结论 慢性酒精中毒对大鼠视功能产生显著损害,抗氧化剂ebselen对视功能具有保护作用,证实氧化应激反应在慢性酒精中毒损害机制中发挥了重要作用.
目的 探討慢性酒精中毒對大鼠視功能的損害以及抗氧化劑ebselen對視功能的保護作用.方法 實驗研究.利用自由飲方法建立慢性酒精中毒大鼠模型,按隨機數字錶將60隻大鼠隨機分為對照組、酒精組和ebselen治療組(即藥物組),採用閃光視網膜電圖(FERG)及閃光視覺誘髮電位(FVEP)觀察大鼠視功能的改變.對數據進行單因素方差分析.結果 3組FERG a、b波潛時差異有統計學意義(F=4.161、3.664,P＜0.05),酒精組a、b波潛時較對照組明顯長(P＜0.05),而藥物組與對照組相比差異無統計學意義(P＞0.05).FERG b波振幅酒精組及藥物組均較對照組低(F=3.312,P＜0.05).3組FVEP N1波、P1波潛時差異有統計學意義(F=7.940、5.07,P＜0.05),與對照組相比,酒精組N1波、P1波潛時明顯較長(P＜0.05).3組FVEP N1-P1波振幅差異有統計學意義(F=3.874,P＜0.05),與對照組相比,N1-P1波振幅明顯較低(P＜0.05).藥物組N1、P1波潛時及N1-P1振幅與對照組相比差異均無統計學意義(P＞0.05).結論 慢性酒精中毒對大鼠視功能產生顯著損害,抗氧化劑ebselen對視功能具有保護作用,證實氧化應激反應在慢性酒精中毒損害機製中髮揮瞭重要作用.
목적 탐토만성주정중독대대서시공능적손해이급항양화제ebselen대시공능적보호작용.방법 실험연구.이용자유음방법건립만성주정중독대서모형,안수궤수자표장60지대서수궤분위대조조、주정조화ebselen치료조(즉약물조),채용섬광시망막전도(FERG)급섬광시각유발전위(FVEP)관찰대서시공능적개변.대수거진행단인소방차분석.결과 3조FERG a、b파잠시차이유통계학의의(F=4.161、3.664,P＜0.05),주정조a、b파잠시교대조조명현장(P＜0.05),이약물조여대조조상비차이무통계학의의(P＞0.05).FERG b파진폭주정조급약물조균교대조조저(F=3.312,P＜0.05).3조FVEP N1파、P1파잠시차이유통계학의의(F=7.940、5.07,P＜0.05),여대조조상비,주정조N1파、P1파잠시명현교장(P＜0.05).3조FVEP N1-P1파진폭차이유통계학의의(F=3.874,P＜0.05),여대조조상비,N1-P1파진폭명현교저(P＜0.05).약물조N1、P1파잠시급N1-P1진폭여대조조상비차이균무통계학의의(P＞0.05).결론 만성주정중독대대서시공능산생현저손해,항양화제ebselen대시공능구유보호작용,증실양화응격반응재만성주정중독손해궤제중발휘료중요작용.
Objective To evaluate the effects of chronic alcoholism on visual function in rats and the protective effects of ebselen.Methods In this experimental study,the models were based on chronic alcohol feeding.Rats were divided randomly into 3 groups:control group,ethanol group and ethanol+ebselen group (drug group).Visual function was assessed by FERG and FVEP.Data were analyzed using ANOVA.Results The latencies of the a-wave and b-wave of FERG were obviously prolonged in the ethanol group compared to the control group (F=4.161,3.664,P＜0.05),while no differences were found between the drug group and control group (P＞0.05).The amplitude of the b-wave decreased in the ethanol and drug groups compared to the control group (F=3.312,P＜0.05).For FVEP,the latencies of the N1 and P1 waves were prolonged and the amplitude of the N1-P1 wave obviously decreased in the ethanol group compared to the control group (F=7.940,5.0707,3.874,P＜0.05),while no differences were found between the drug group and control group (P＞0.05).Conclusion The damage to the visual system from chronic alcoholism can be prevented by an antioxidant.Thus,it can be inferred that oxidative stress plays a significant role in the damage from chronic alcoholism in the central nervous system.
