中华眼视光学与视觉科学杂志
中華眼視光學與視覺科學雜誌
중화안시광학여시각과학잡지
CHINESE JOURNAL OF OPTOMETRY OPHTHALMOLOGY AND VISUAL SCIENCE
2014年
5期
279-281
,共3页
张亚丽%李志伟%牟国营%刘蕾
張亞麗%李誌偉%牟國營%劉蕾
장아려%리지위%모국영%류뢰
生物力学%巩膜%核黄素%紫外线%胶原交联
生物力學%鞏膜%覈黃素%紫外線%膠原交聯
생물역학%공막%핵황소%자외선%효원교련
Biomechanics%Sclera%Riboflavin%Ultraviolet%Collagen crosslinking
目的 比较兔和人巩膜核黄素/紫外线A交联前后的生物力学性能.方法 实验研究.取8只成年新西兰大白兔和8只新鲜离体成人眼,沿矢状位方向平均分为两半,一半作为对照,一半作交联试件.交联的参数设置:0.1％核黄素溶液滴眼,每3 min 1次,共5次;紫外线波长365 nm;能量密度3 mW/cm2;照射时间40 min.生物力学测试指标:极限应力、8％应变条件下的应力和弹性模量.应用t检验对交联前后的生物力学指标进行统计分析.结果 8％应变时兔和人巩膜应力分别为(359±305)kPa和(1 079±646)kPa;极限应力分别为(3 830±2 870)kPa和(4450±1 120)kPa;弹性模量分别为(4 460±4090)kPa和(14 310±8 560)kPa.交联后兔巩膜在应力、极限应力和弹性模量上均有显著性提高(t=3.07、3.50、3.04,P＜0.05);而人巩膜交联前后的生物力学指标差异均无统计学意义.结论 人巩膜生物力学性能高于兔;胶原交联可显著增加兔巩膜生物力学性能,而在相同条件下人巩膜交联前后生物力学强度无改变.
目的 比較兔和人鞏膜覈黃素/紫外線A交聯前後的生物力學性能.方法 實驗研究.取8隻成年新西蘭大白兔和8隻新鮮離體成人眼,沿矢狀位方嚮平均分為兩半,一半作為對照,一半作交聯試件.交聯的參數設置:0.1％覈黃素溶液滴眼,每3 min 1次,共5次;紫外線波長365 nm;能量密度3 mW/cm2;照射時間40 min.生物力學測試指標:極限應力、8％應變條件下的應力和彈性模量.應用t檢驗對交聯前後的生物力學指標進行統計分析.結果 8％應變時兔和人鞏膜應力分彆為(359±305)kPa和(1 079±646)kPa;極限應力分彆為(3 830±2 870)kPa和(4450±1 120)kPa;彈性模量分彆為(4 460±4090)kPa和(14 310±8 560)kPa.交聯後兔鞏膜在應力、極限應力和彈性模量上均有顯著性提高(t=3.07、3.50、3.04,P＜0.05);而人鞏膜交聯前後的生物力學指標差異均無統計學意義.結論 人鞏膜生物力學性能高于兔;膠原交聯可顯著增加兔鞏膜生物力學性能,而在相同條件下人鞏膜交聯前後生物力學彊度無改變.
목적 비교토화인공막핵황소/자외선A교련전후적생물역학성능.방법 실험연구.취8지성년신서란대백토화8지신선리체성인안,연시상위방향평균분위량반,일반작위대조,일반작교련시건.교련적삼수설치:0.1％핵황소용액적안,매3 min 1차,공5차;자외선파장365 nm;능량밀도3 mW/cm2;조사시간40 min.생물역학측시지표:겁한응력、8％응변조건하적응력화탄성모량.응용t검험대교련전후적생물역학지표진행통계분석.결과 8％응변시토화인공막응력분별위(359±305)kPa화(1 079±646)kPa;겁한응력분별위(3 830±2 870)kPa화(4450±1 120)kPa;탄성모량분별위(4 460±4090)kPa화(14 310±8 560)kPa.교련후토공막재응력、겁한응력화탄성모량상균유현저성제고(t=3.07、3.50、3.04,P＜0.05);이인공막교련전후적생물역학지표차이균무통계학의의.결론 인공막생물역학성능고우토;효원교련가현저증가토공막생물역학성능,이재상동조건하인공막교련전후생물역학강도무개변.
Objective To compare the biomechanical properties of rabbit and human sclera before and after riboflavin/ultraviolet A (UVA) collagen crosslinking.Methods Experimental study.Eight rabbits and 8 human eyeballs were included.The eyes were bisected,one half was cross-linked with riboflavin/UVA (photosensitizer:0.1％ riboflavin drops for 15 minutes,UVA:3 mW/cm2,365 nm,and 40 minutes) and the other half served as control.Biomechanical properties testing consisted of ultimate stress,stress and Young's modulus at 8％ strain.Data were analyzed using t test.Results In untreated rabbit and human sclera,Young's modulus at 8％ strain was (4 460±4 090)kPa and (14310±8560)kPa,the stress was (359±305)kPa and (1 079±646)kPa,the ultimate stress was (3 830±2 870)kPa and (4 450±1 120)kPa.In rabbit sclera,the stress,ultimate stress and Young's modulus were significantly increased by riboflavin/UVA crosslinking (t=3.07,3.50,3.04,P＜0.05).The biomechanical properties of human sclera were not statistically affected by crosslinking.Conclusion Human sclera has a higher biomechanical stiffness than rabbit sclera.With the same irradiation dose,riboflavin/UVA crosslinking increases the biomechanical stiffness of rabbit sclera,but not human sclera.
