CAJ | 학술논문

目的探讨微RNA-21 (miR-21)在神经胶质瘤患者脑脊液中的水平及其临床意义.方法采用实时定量反转录聚合酶链反应(RT-PCR)检测40例神经胶质瘤患者(神经胶质瘤组)及40例健康人(对照组)脑脊液中miR-21水平,并分析miR-21水平与神经胶质瘤病理分级之间的关系.结果神经胶质瘤组脑脊液中miR-21相对表达水平为0.70±0.12,与对照组的0.43±0.09比较明显升高,差异有统计学意义(t=11.3840,P＜ 0.01).神经胶质瘤病理分级Ⅱ、Ⅲ、Ⅳ级患者分别有16、13、11例,脑脊液中miR-21相对表达水平分别为0.66±0.11、0.71±0.12、0.74±0.13,差异无统计学意义(F=1.5083,P=0.2345).结论神经胶质瘤患者脑脊液中miR-21水平增高,miR-21可能参与了神经胶质瘤的发生、发展过程.
목적 탐토미RNA-21 (miR-21)재신경효질류환자뇌척액중적수평급기림상의의.방법 채용실시정량반전록취합매련반응(RT-PCR)검측40례신경효질류환자(신경효질류조)급40례건강인(대조조)뇌척액중miR-21수평,병분석miR-21수평여신경효질류병리분급지간적관계.결과 신경효질류조뇌척액중miR-21상대표체수평위0.70±0.12,여대조조적0.43±0.09비교명현승고,차이유통계학의의(t=11.3840,P＜ 0.01).신경효질류병리분급Ⅱ、Ⅲ、Ⅳ급환자분별유16、13、11례,뇌척액중miR-21상대표체수평분별위0.66±0.11、0.71±0.12、0.74±0.13,차이무통계학의의(F=1.5083,P=0.2345).결론 신경효질류환자뇌척액중miR-21수평증고,miR-21가능삼여료신경효질류적발생、발전과정.
Objective To explore the level and its clinical significance of microRNA-21 (miR-21) in cerebrospinal fluid of glioma patients.Methods The level of miR-21 in cerebrospinal fluid were measured by real-time RT-PCR in 40 patients with glioma (glioma group) and 40 normal controls(control group).The relationship between miR-21 and pathological grade were analyzed.Results The level of miR-21 in glioma group was significantly higher than that in control group (0.70 + 0.12 vs.0.43 + 0.09,t =11.3840,P ＜ 0.01).The level of miR-21 in glioma group with Ⅱ,Ⅲ and Ⅳ pathological grade was 0.66 +0.11,0.71 + 0.12,0.74 ± 0.13,there was no significant difference (F =1.5083,P =0.2345).Conclusions The level of miR-21 in cerebrospinal fluid of glioma patients elevates significantly.It may participate in the occurrence and development of glioma.