中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2013年
16期
37-39
,共3页
癫痫%丙戊酸%中性粒细胞
癲癇%丙戊痠%中性粒細胞
전간%병무산%중성립세포
Epilepsy%Valproic acid%Neutrophil
目的 检测服用丙戊酸钠治疗的癫痫患儿体内中性粒细胞活性的改变,并探讨其相关的临床意义.方法 收集服用丙戊酸钠治疗的癫痫患儿34例作为癫痫组,另选择同期健康体检儿童30例作为对照组.采用流式细胞仪检测中性粒细胞活化率.乳胶增强免疫比浊法检测超敏C反应蛋白(hs-CRP)水平,酶联免疫吸附试验法检测肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)水平.结果 癫痫组治疗前中性粒细胞活化率与对照组比较差异无统计学意义(P＞0.05),癫痫组治疗6、12个月中性粒细胞活化率均显著高于对照组[(12.36±4.72)％、(15.87±5.68)％比(5.32±1.41)％,P＜ 0.01],且治疗12个月中性粒细胞活化率高于治疗6个月(P＜0.05).癫痫组治疗前血清hs-CRP、TNF-α和IL-6水平与对照组比较差异无统计学意义(P＞0.05),癫痫组治疗6、12个月血清hs-CRP、TNF-α和IL-6水平显著高于对照组[(3.64±1.22)、(6.96±2.64) mg/L比(1.46±0.27)mg/L,(74.72±22.58)、(96.67±30.25) ng/L比(31.72±12.16) ng/L,(32.59±8.45)、(46.74±12.16)ng/L比(15.36±4.45) ng/L,P＜ 0.01],且治疗12个月血清hs-CRP、TNF-α和IL-6水平显著高于治疗6个月,差异有统计学意义(P＜0.05).癫痫患儿中性粒细胞活化率与血清hs-CRP、TNF-α和IL-6水平均呈正相关(r=0.328、0.402、0.344,P＜ 0.05).结论 丙戊酸钠治疗的癫痫患儿体内中性粒细胞活化率出现了显著的升高,中性粒细胞的异常活化使机体处于高氧化应激状态.
目的 檢測服用丙戊痠鈉治療的癲癇患兒體內中性粒細胞活性的改變,併探討其相關的臨床意義.方法 收集服用丙戊痠鈉治療的癲癇患兒34例作為癲癇組,另選擇同期健康體檢兒童30例作為對照組.採用流式細胞儀檢測中性粒細胞活化率.乳膠增彊免疫比濁法檢測超敏C反應蛋白(hs-CRP)水平,酶聯免疫吸附試驗法檢測腫瘤壞死因子α(TNF-α)和白細胞介素6(IL-6)水平.結果 癲癇組治療前中性粒細胞活化率與對照組比較差異無統計學意義(P＞0.05),癲癇組治療6、12箇月中性粒細胞活化率均顯著高于對照組[(12.36±4.72)％、(15.87±5.68)％比(5.32±1.41)％,P＜ 0.01],且治療12箇月中性粒細胞活化率高于治療6箇月(P＜0.05).癲癇組治療前血清hs-CRP、TNF-α和IL-6水平與對照組比較差異無統計學意義(P＞0.05),癲癇組治療6、12箇月血清hs-CRP、TNF-α和IL-6水平顯著高于對照組[(3.64±1.22)、(6.96±2.64) mg/L比(1.46±0.27)mg/L,(74.72±22.58)、(96.67±30.25) ng/L比(31.72±12.16) ng/L,(32.59±8.45)、(46.74±12.16)ng/L比(15.36±4.45) ng/L,P＜ 0.01],且治療12箇月血清hs-CRP、TNF-α和IL-6水平顯著高于治療6箇月,差異有統計學意義(P＜0.05).癲癇患兒中性粒細胞活化率與血清hs-CRP、TNF-α和IL-6水平均呈正相關(r=0.328、0.402、0.344,P＜ 0.05).結論 丙戊痠鈉治療的癲癇患兒體內中性粒細胞活化率齣現瞭顯著的升高,中性粒細胞的異常活化使機體處于高氧化應激狀態.
목적 검측복용병무산납치료적전간환인체내중성립세포활성적개변,병탐토기상관적림상의의.방법 수집복용병무산납치료적전간환인34례작위전간조,령선택동기건강체검인동30례작위대조조.채용류식세포의검측중성립세포활화솔.유효증강면역비탁법검측초민C반응단백(hs-CRP)수평,매련면역흡부시험법검측종류배사인자α(TNF-α)화백세포개소6(IL-6)수평.결과 전간조치료전중성립세포활화솔여대조조비교차이무통계학의의(P＞0.05),전간조치료6、12개월중성립세포활화솔균현저고우대조조[(12.36±4.72)％、(15.87±5.68)％비(5.32±1.41)％,P＜ 0.01],차치료12개월중성립세포활화솔고우치료6개월(P＜0.05).전간조치료전혈청hs-CRP、TNF-α화IL-6수평여대조조비교차이무통계학의의(P＞0.05),전간조치료6、12개월혈청hs-CRP、TNF-α화IL-6수평현저고우대조조[(3.64±1.22)、(6.96±2.64) mg/L비(1.46±0.27)mg/L,(74.72±22.58)、(96.67±30.25) ng/L비(31.72±12.16) ng/L,(32.59±8.45)、(46.74±12.16)ng/L비(15.36±4.45) ng/L,P＜ 0.01],차치료12개월혈청hs-CRP、TNF-α화IL-6수평현저고우치료6개월,차이유통계학의의(P＜0.05).전간환인중성립세포활화솔여혈청hs-CRP、TNF-α화IL-6수평균정정상관(r=0.328、0.402、0.344,P＜ 0.05).결론 병무산납치료적전간환인체내중성립세포활화솔출현료현저적승고,중성립세포적이상활화사궤체처우고양화응격상태.
Objective To investigate the effect ofvalproate acid(VAP) on the activity ofneutrophils in children with epilepsy and its clinical significance.Methods A total of 34 cases of children with epilepsy(epilepsy group) were enrolled in this study.Another 30 healthy children were as control group.The activation rate of neutrophils was detected by flow cytometry.The levels of serum high sensitivity-C reactive protein(hs-CRP) were measured by latex enhanced immunoturbidimetric analysis.The levels of serum tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were measured by enzyme linked immunosorbent assay analysis.Results The activation rate of neutrophils had no significant difference between epilepsy group before treatment and control group (P ＞ 0.05).The activation rate of neutrophils after treatment of 6,12 months in epilepsy group was higher than that in control group [(12.36 ± 4.72)％,(15.87 ± 5.68)％ vs.(5.32 ± 1.41)％,P ＜ 0.01].The activation rate of neutrophils after treatment of 12 months was higher than that after treatment of 6 months in epilepsy group (P ＜ 0.05).There was no significant difference in the levels of serum hs-CRP,TNF-α and IL-6 between epilepsy group before treatment and control group (P ＞ 0.05).The levels of serum hs-CRP,TNF-α and IL-6 after treatment of 6,12 months in epilepsy group were higher than those in control group [(3.64 ± 1.22),(6.96 ± 2.64) mg/L vs.(1.46 ± 0.27) mg/L,(74.72 ± 22.58),(96.67 ± 30.25) ng/L vs.(31.72 ± 12.16) ng/L,(32.59 ± 8.45),(46.74 ± 12.16) ng/L vs.(15.36 ± 4.45)ng/L,P ＜ 0.01],and those after treatment of 12 months were higher than those after treatment of 6 months (P ＜ 0.05).The activation rate of neutrophils was positively related with the serum levels of hs-CRP,TNF-αand IL-6 (r =0.328,0.402,0.344,P ＜ 0.05).Conclusions The high activation rate of neutrophils is found in children with epilepsy.The abnormal activation of neutrophils makes the body undergo high oxidative stress status.
