中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2013年
25期
30-32
,共3页
金培声%任宗海%叶方鹏%应卫星
金培聲%任宗海%葉方鵬%應衛星
금배성%임종해%협방붕%응위성
胰腺炎%细胞因子类%早期诊断
胰腺炎%細胞因子類%早期診斷
이선염%세포인자류%조기진단
Pancreatitis%Cytokines%Early diagnosis
目的 探讨血清多种细胞因子同时检测对急性胰腺炎(AP)早期诊断和病情评估的价值.方法 选择符合AP诊断标准的患者48例,依据CT诊断结果分为轻症急性胰腺炎(MAP)组30例和重症急性胰腺炎(SAP)组18例,另选择30例健康体检者作为对照组.采用酶联免疫吸附试验(ELISA)方法定量检测患者血清中多种细胞因子水平.结果 MAP组血清白细胞介素(IL)-1、IL-10和肿瘤坏死因子(TNF)-α水平分别为(25.00±1.92)、(59.78±4.51)、(55.31±8.54)ng/L,显著高于对照组的(10.08±2.65)、(1.80±0.66)、(18.72±7.84) ng/L,差异有统计学意义(P＜ 0.05);SAP组血清IL-I、IL-6、IL-8、TNF-α和血小板活化因子(PAF)水平分别为(93.27±3.98)、(397.84±13.05)、(93.32±3.40)、(181.94±7.54)、(284.53±7.88) ng/L,显著高于MAP组[(25.00±1.92)、(34.12±4.96)、(13.06±1.86)、(55.31±8.54)、(175.25±30.15) ng/L],差异有统计学意义(P＜0.05).结论 血清多种细胞因子的检测对AP的早期诊断和鉴别MAP与SAP具有重要的价值.
目的 探討血清多種細胞因子同時檢測對急性胰腺炎(AP)早期診斷和病情評估的價值.方法 選擇符閤AP診斷標準的患者48例,依據CT診斷結果分為輕癥急性胰腺炎(MAP)組30例和重癥急性胰腺炎(SAP)組18例,另選擇30例健康體檢者作為對照組.採用酶聯免疫吸附試驗(ELISA)方法定量檢測患者血清中多種細胞因子水平.結果 MAP組血清白細胞介素(IL)-1、IL-10和腫瘤壞死因子(TNF)-α水平分彆為(25.00±1.92)、(59.78±4.51)、(55.31±8.54)ng/L,顯著高于對照組的(10.08±2.65)、(1.80±0.66)、(18.72±7.84) ng/L,差異有統計學意義(P＜ 0.05);SAP組血清IL-I、IL-6、IL-8、TNF-α和血小闆活化因子(PAF)水平分彆為(93.27±3.98)、(397.84±13.05)、(93.32±3.40)、(181.94±7.54)、(284.53±7.88) ng/L,顯著高于MAP組[(25.00±1.92)、(34.12±4.96)、(13.06±1.86)、(55.31±8.54)、(175.25±30.15) ng/L],差異有統計學意義(P＜0.05).結論 血清多種細胞因子的檢測對AP的早期診斷和鑒彆MAP與SAP具有重要的價值.
목적 탐토혈청다충세포인자동시검측대급성이선염(AP)조기진단화병정평고적개치.방법 선택부합AP진단표준적환자48례,의거CT진단결과분위경증급성이선염(MAP)조30례화중증급성이선염(SAP)조18례,령선택30례건강체검자작위대조조.채용매련면역흡부시험(ELISA)방법정량검측환자혈청중다충세포인자수평.결과 MAP조혈청백세포개소(IL)-1、IL-10화종류배사인자(TNF)-α수평분별위(25.00±1.92)、(59.78±4.51)、(55.31±8.54)ng/L,현저고우대조조적(10.08±2.65)、(1.80±0.66)、(18.72±7.84) ng/L,차이유통계학의의(P＜ 0.05);SAP조혈청IL-I、IL-6、IL-8、TNF-α화혈소판활화인자(PAF)수평분별위(93.27±3.98)、(397.84±13.05)、(93.32±3.40)、(181.94±7.54)、(284.53±7.88) ng/L,현저고우MAP조[(25.00±1.92)、(34.12±4.96)、(13.06±1.86)、(55.31±8.54)、(175.25±30.15) ng/L],차이유통계학의의(P＜0.05).결론 혈청다충세포인자적검측대AP적조기진단화감별MAP여SAP구유중요적개치.
Objective To investigate the early diagnosis and disease evaluation value in patients with acute pancreatitis by various serum cytokines measurement.Methods Forty-eight acute pancreatitis patients were divided into two groups based on the results of computed tomography (CT) examination:mild acute pancreatitis group (30 cases) and severe acute pancreatitis group (18 cases).The other 30 normal persons were selected as control group.The various serum cytokines were measured by enzyme-linked immunosorbent assay (ELISA).Results The serum concentrations of interleukin(IL)-1,IL-10 and tumor necrosis factor (TNF)-α in mild acute pancreatitis group were significantly higher than those in control group [(25.00 ± 1.92) ng/L vs.(10.08 ± 2.65) ng/L,(59.78 ± 4.51) ng/L vs.(1.80 ± 0.66) ng/L,(55.31 ± 8.54) ng/L vs.(18.72 ± 7.84) ng/L,P ＜ 0.05].The serum concentrations of IL-1,IL-6,IL-8,TNF-α and platelet activating factor (PAF) in severe acute pancreatitis group were significantly higher than those in mild acute pancreatitis group [(93.27 ± 3.98) ng/L vs.(25.00 ± 1.92) ng/L,(397.84 ± 13.05) ng/L vs.(34.12 ± 4.96) ng/L,(93.32 ±3.40) ng/Lvs.(13.06± 1.86) ng/L,(181.94 ±7.54) ng/Lvs.(55.31 ±8.54) ng/L,(284.53 ±7.88) ng/L vs.(175.25 ±30.15) ng/L,P＜0.05].Conclusion The various serum cytokines measurement has great importance on the early diagnosis of acute pancreatitis and discrimination between the mild acute pancreatitis and severe acute pancreatitis.
