CAJ | 학술논문

目的探讨E3泛素连接酶Iduna mRNA和蛋白在非小细胞肺癌组织和癌旁肺组织中的表达及其与临床病理因素和预后的关系.方法应用反转录聚合酶链反应(RT-PCR)、Western-Blot和免疫组织化学(EnVision)方法检测分析非小细胞肺癌组织和癌旁肺组织中Iduna mRNA和蛋白的表达,并分析其与临床病理因素和预后的关系.结果 RT-PCR及Western-Blot结果显示非小细胞肺癌组织中Iduna mRNA和蛋白表达水平分别为0.468±0.086和2.554±0.544,明显高于癌旁肺组织的0.203±0.070和1.570±0.316,差异均有统计学意义(P＜0.05);免疫组织化学结果显示:Iduna在肺泡上皮细胞中呈阴性,在正常支气管黏膜上皮中呈阴性或弱阳性,在非小细胞肺癌组织中呈不同程度的阳性表达.Iduna的高表达率与肿瘤分化程度呈负相关(P=0.002),与肿瘤大小(P=0.044)、TNM分期(P=0.015)、淋巴结转移(P=0.009)呈正相关,并与Ⅰ期非小细胞肺癌患者的预后有关(P=0.016).结论 Iduna的高表达可能与非小细胞肺癌的侵袭、转移过程密切相关,有可能是提示非小细胞肺癌患者预后不良的指标.
목적 탐토E3범소련접매Iduna mRNA화단백재비소세포폐암조직화암방폐조직중적표체급기여림상병리인소화예후적관계.방법 응용반전록취합매련반응(RT-PCR)、Western-Blot화면역조직화학(EnVision)방법검측분석비소세포폐암조직화암방폐조직중Iduna mRNA화단백적표체,병분석기여림상병리인소화예후적관계.결과 RT-PCR급Western-Blot결과현시비소세포폐암조직중Iduna mRNA화단백표체수평분별위0.468±0.086화2.554±0.544,명현고우암방폐조직적0.203±0.070화1.570±0.316,차이균유통계학의의(P＜0.05);면역조직화학결과현시:Iduna재폐포상피세포중정음성,재정상지기관점막상피중정음성혹약양성,재비소세포폐암조직중정불동정도적양성표체.Iduna적고표체솔여종류분화정도정부상관(P=0.002),여종류대소(P=0.044)、TNM분기(P=0.015)、림파결전이(P=0.009)정정상관,병여Ⅰ기비소세포폐암환자적예후유관(P=0.016).결론 Iduna적고표체가능여비소세포폐암적침습、전이과정밀절상관,유가능시제시비소세포폐암환자예후불량적지표.
Objective To investigate the mRNA and protein expression of E3 ubiquitin ligase Iduna in non-small-cell lung cancer tissue and para-neoplastic lung tissue,and the correlation of the Iduna expression with clinicopathological factors and prognosis.Methods The expression levels of the Iduna mRNA and protein in non-small-cell lung cancer tissue and para-neoplastic lung tissue were determined by reverse transcriptase-polymerase chain reaction (RT-PCR),Western-Blot and immunohistochemistry respectively,and the correlation of the Iduna expression with clinicopathological factors and prognosis was analyzed.Results RT-PCR and Western-Blot showed the expression levels of the Iduna mRNA and protein in non-small-cell lung cancer tissue (0.468 ± 0.086 and 2.554 ± 0.544) were significantly higher than those in para-neoplastic lung tissue (0.203 ± 0.070 and 1.570 ± 0.316),there were statistical differences (P ＜ 0.05).Immunohistochemistry results showed that Iduna was negative expression in the alveolar epithelium cells,negative or weak positive expression in normal bronchial and positive expression in different degrees in the non-small-cell lung cancer tissue.Iduna high expression rate was negative correlation with tumor differentiation (P =0.002),Iduna high expression rate was positive correlation with large tumor size (P =0.044),TNM staging (P=0.015) and lymph node metastasis (P=0.009).Iduna high expression of I stage non-small-cell lung cancer patients was correlated with poor post-operative survival (P =0.016).Conclusions High expression of Iduna may be related to the process of invasion and metastasis of nonsmall-cell lung cancer.It is possible that Iduna serve as potential markers for predicting prognosis in nonsmall-cell lung cancer.