中华眼外伤职业眼病杂志
中華眼外傷職業眼病雜誌
중화안외상직업안병잡지
CHINESE JOURNAL OF OCULAR TRAUMA AND OCCUPATIONAL EYE DISEASE
2013年
11期
801-804
,共4页
损伤,视神经%牛磺酸%一氧化氮合酶,诱导型
損傷,視神經%牛磺痠%一氧化氮閤酶,誘導型
손상,시신경%우광산%일양화담합매,유도형
Injury,optic nerve%Taurine%Oxide synthase,nitric,inducible
目的 研究大鼠视神经挫伤后诱导型一氧化氮合酶(iNOS)的变化及牛磺酸的影响.方法 通过建立外伤性视神经损伤的动物模型,随机分为正常对照组、损伤组(视神经钳夹+蒸馏水组)及牛磺酸组(视神经钳夹+牛磺酸组),于损伤后3d、7d、14 d和28 d以免疫组化染色法测定视神经iNOS的活性.结果 在损伤后3d、7d、14 d及28 d,损伤组及牛磺酸组视神经组织iNOS表达较正常对照组升高(F=256.43,213.62,188.76,231.78,P＜0.05);牛磺酸组较损伤组iNOS表达降低(F =256.43,213.62,188.76,231.78,P＜0.05).结论 视神经损伤诱导视神经iNOS的表达.牛磺酸可能通过对iNOS的抑制在大鼠视神经损伤中起到视神经保护作用.
目的 研究大鼠視神經挫傷後誘導型一氧化氮閤酶(iNOS)的變化及牛磺痠的影響.方法 通過建立外傷性視神經損傷的動物模型,隨機分為正常對照組、損傷組(視神經鉗夾+蒸餾水組)及牛磺痠組(視神經鉗夾+牛磺痠組),于損傷後3d、7d、14 d和28 d以免疫組化染色法測定視神經iNOS的活性.結果 在損傷後3d、7d、14 d及28 d,損傷組及牛磺痠組視神經組織iNOS錶達較正常對照組升高(F=256.43,213.62,188.76,231.78,P＜0.05);牛磺痠組較損傷組iNOS錶達降低(F =256.43,213.62,188.76,231.78,P＜0.05).結論 視神經損傷誘導視神經iNOS的錶達.牛磺痠可能通過對iNOS的抑製在大鼠視神經損傷中起到視神經保護作用.
목적 연구대서시신경좌상후유도형일양화담합매(iNOS)적변화급우광산적영향.방법 통과건립외상성시신경손상적동물모형,수궤분위정상대조조、손상조(시신경겸협+증류수조)급우광산조(시신경겸협+우광산조),우손상후3d、7d、14 d화28 d이면역조화염색법측정시신경iNOS적활성.결과 재손상후3d、7d、14 d급28 d,손상조급우광산조시신경조직iNOS표체교정상대조조승고(F=256.43,213.62,188.76,231.78,P＜0.05);우광산조교손상조iNOS표체강저(F =256.43,213.62,188.76,231.78,P＜0.05).결론 시신경손상유도시신경iNOS적표체.우광산가능통과대iNOS적억제재대서시신경손상중기도시신경보호작용.
Objective To study changes of inducible nitric oxide synthase (iNOS) and effect of taurine in rats with optic nerve contusion.Methods The model of traumatic optic neuropathy was established,Three groups were divided randomly:control group,crush group (optic nerve crushing + distilled water) and taurine group (optic nerve crushing + taurine).Activity of iNOS in optic nerve at 3 days,7 days,14 days and 28 days after crushing were determined with immunohistochemistry staining.Results At 3 days,7 days,14 days and 28 days after crushing,the expression of iNOS in optic nerve increased in crush group and taurine group compared with control group (F =256.43,213.62,188.76,231.78,P ＜0.05) ; the expression of iNOS in taurine group decreased compared with crush group (F=256.43,213.62,188.76,231.78,P ＜ 0.05).Conclusion Optic nerve injury could induce the expression of iNOS in optic nerve.By inhibiting the activity of iNOS,taurine might protect the injured optic nerve in rats with traumatic optic neuropathy.
