中华眼外伤职业眼病杂志
中華眼外傷職業眼病雜誌
중화안외상직업안병잡지
CHINESE JOURNAL OF OCULAR TRAUMA AND OCCUPATIONAL EYE DISEASE
2014年
5期
321-325
,共5页
姜严明%梁歌%陈鹏%黄一飞%梁洁
薑嚴明%樑歌%陳鵬%黃一飛%樑潔
강엄명%량가%진붕%황일비%량길
532 nm激光%低剂量%大鼠模型%眼损伤%眼电生理
532 nm激光%低劑量%大鼠模型%眼損傷%眼電生理
532 nm격광%저제량%대서모형%안손상%안전생리
Laser,532nm%Dose,low%Model,animal,rat%Injury,ocular%Examination,electrophysiology,visual
目的 建立有效、可控、简便、重复性好的弥散性低剂量532 nm激光眼损伤大鼠模型.方法 采用相应激光器和自制装置造模,将实验动物分为照射后即刻、1d、3d、5d、7d和14 d组.用直接检眼镜、彩色眼底照相、荧光素眼底血管造影观察受照后大鼠的眼底改变,行视网膜电流图检查.结果 建立了无肉眼可见的损伤的、弥散性532 nm激光照射的动物模型.此模型中眼底照相及荧光素眼底血管造影均未见异常.视网膜电流图显示,随照射时间的增加,暗适应b波振幅明显降低,暗适应眼的最大混合反应a、b波潜伏期延长,振幅降低,明适应视锥细胞反应a、b波振幅明显降低(P＜0.05);振荡电位无明显变化(P＞0.05);结论 本研究建立了有效、可控制、简便易行、重复性好的低剂量激光重复辐照致眼损伤的大鼠模型.在并未对大鼠的视网膜造成肉眼可见的损伤的情况下,造模组视网膜电生理活动减弱程度与照射时间的延长密切相关.提示在低剂量激光重复照射致眼损伤的临床诊断及对预后的评估中,眼电生理检查应被列为重要的一项辅助检查.受照7d至14d为激光眩目眼损伤致伤效应的平台期.
目的 建立有效、可控、簡便、重複性好的瀰散性低劑量532 nm激光眼損傷大鼠模型.方法 採用相應激光器和自製裝置造模,將實驗動物分為照射後即刻、1d、3d、5d、7d和14 d組.用直接檢眼鏡、綵色眼底照相、熒光素眼底血管造影觀察受照後大鼠的眼底改變,行視網膜電流圖檢查.結果 建立瞭無肉眼可見的損傷的、瀰散性532 nm激光照射的動物模型.此模型中眼底照相及熒光素眼底血管造影均未見異常.視網膜電流圖顯示,隨照射時間的增加,暗適應b波振幅明顯降低,暗適應眼的最大混閤反應a、b波潛伏期延長,振幅降低,明適應視錐細胞反應a、b波振幅明顯降低(P＜0.05);振盪電位無明顯變化(P＞0.05);結論 本研究建立瞭有效、可控製、簡便易行、重複性好的低劑量激光重複輻照緻眼損傷的大鼠模型.在併未對大鼠的視網膜造成肉眼可見的損傷的情況下,造模組視網膜電生理活動減弱程度與照射時間的延長密切相關.提示在低劑量激光重複照射緻眼損傷的臨床診斷及對預後的評估中,眼電生理檢查應被列為重要的一項輔助檢查.受照7d至14d為激光眩目眼損傷緻傷效應的平檯期.
목적 건립유효、가공、간편、중복성호적미산성저제량532 nm격광안손상대서모형.방법 채용상응격광기화자제장치조모,장실험동물분위조사후즉각、1d、3d、5d、7d화14 d조.용직접검안경、채색안저조상、형광소안저혈관조영관찰수조후대서적안저개변,행시망막전류도검사.결과 건립료무육안가견적손상적、미산성532 nm격광조사적동물모형.차모형중안저조상급형광소안저혈관조영균미견이상.시망막전류도현시,수조사시간적증가,암괄응b파진폭명현강저,암괄응안적최대혼합반응a、b파잠복기연장,진폭강저,명괄응시추세포반응a、b파진폭명현강저(P＜0.05);진탕전위무명현변화(P＞0.05);결론 본연구건립료유효、가공제、간편역행、중복성호적저제량격광중복복조치안손상적대서모형.재병미대대서적시망막조성육안가견적손상적정황하,조모조시망막전생리활동감약정도여조사시간적연장밀절상관.제시재저제량격광중복조사치안손상적림상진단급대예후적평고중,안전생리검사응피렬위중요적일항보조검사.수조7d지14d위격광현목안손상치상효응적평태기.
Objective To establish effective,controllable,simple and well repeatable rat eye model injured by diffuse low dose 532 nm laser.Methods The animal models in rats were made by selfmade laser device and apparatus.These rats were divided into six groups according to their exposure time:instant,1 d,3 d,5 d,7 d and 14 d.After exposure,the rats were examined by direct ophthalmoscopy,fundus photography,fundus flurescein angiography and electroretinogram.Results The rat eye model injured by diffuse low dose 532 nm laser was successfully established.The results of direct ophthalmoscopy,fundus photography,fundus flurescein angiography in these models were normal.As shown by electroretinogram,with laser exposure time expending,0.01 rod response of b wave amplitude decreases,0.3 max response of a and b waves amplitude decreased,0.3 cone response of a and b waves incubation period extended and amplitude decreased significantly (P ＜ 0.05).There was no significant difference in oscillatorypotentials(P ＞ 0.05).Conclusion The rat eye model injured by low dose laser is effective,controllable,simple and well repeatable.With the laser exposure time expending,the retinal electrophysiological activity decreases.It shows the visual electrophysiological examination is important for evaluation of eye injury due to low dose laser.The platean period is 7 d to 14 d after laser exposure.
