中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2012年
6期
384-387
,共4页
方春芸%唐国都%梁志海%唐曦平
方春蕓%唐國都%樑誌海%唐晞平
방춘예%당국도%량지해%당희평
胰腺炎,急性坏死性%受体,内源性物质%生长激素促分泌物受体%Ghrelin
胰腺炎,急性壞死性%受體,內源性物質%生長激素促分泌物受體%Ghrelin
이선염,급성배사성%수체,내원성물질%생장격소촉분비물수체%Ghrelin
Pancreatitis,acute necrotizing%Receptors,endogenous substances%Growth hormone secretagogue recepter%Ghrelin
目的 观察急性坏死性胰腺炎(ANP)大鼠胰腺组织ghrelin以及生长激素促分泌素受体(GHSR)的表达,探讨ghrelin在ANP发病机制中的作用.方法 70只大鼠按完全随机法分为ANP组和对照组,每组35只.采用逆行胆胰管注射4％牛磺胆酸钠的方法制备大鼠ANP模型；对照组仅开腹,轻翻动胰腺.术后3、6、12、24、48 h处死大鼠,取血测血清淀粉酶,取胰腺行常规病理检查并评分,应用RTPCR和蛋白质印迹法检测胰腺组织ghrelin、GHSR的mRNA及蛋白表达.结果 ANP组大鼠血清淀粉酶浓度从造模后3h开始升高,6h达(8244±2950) U/L,显著高于对照组(P＜0.05)；胰腺病理损伤及评分随时间延长逐渐加重,24h评分为(11.91 ±1.31)分,显著高于对照组的(3.12±1.60)分.ANP组大鼠胰腺组织ghrelin mRNA和GHSR mRNA表达随时间的延长而逐渐升高,48 h达峰值,分别为1.29 ±0.64和0.94±0.16,均显著高于对照组的0.58 ±0.05和0.19±0.03(P值均＜0.05).ANP组大鼠胰腺组织ghrelin和GHSR蛋白的表达在48 h时达峰值,分别为3.05±0.48和2.34±0.32,均显著高于对照组的2.18±0.23和1.55 ±0.10(P值均＜0.05).结论 ANP大鼠胰腺组织ghrelin、GHSR的mRNA及蛋白表达均显著增加,且与胰腺病变严重程度有关.
目的 觀察急性壞死性胰腺炎(ANP)大鼠胰腺組織ghrelin以及生長激素促分泌素受體(GHSR)的錶達,探討ghrelin在ANP髮病機製中的作用.方法 70隻大鼠按完全隨機法分為ANP組和對照組,每組35隻.採用逆行膽胰管註射4％牛磺膽痠鈉的方法製備大鼠ANP模型；對照組僅開腹,輕翻動胰腺.術後3、6、12、24、48 h處死大鼠,取血測血清澱粉酶,取胰腺行常規病理檢查併評分,應用RTPCR和蛋白質印跡法檢測胰腺組織ghrelin、GHSR的mRNA及蛋白錶達.結果 ANP組大鼠血清澱粉酶濃度從造模後3h開始升高,6h達(8244±2950) U/L,顯著高于對照組(P＜0.05)；胰腺病理損傷及評分隨時間延長逐漸加重,24h評分為(11.91 ±1.31)分,顯著高于對照組的(3.12±1.60)分.ANP組大鼠胰腺組織ghrelin mRNA和GHSR mRNA錶達隨時間的延長而逐漸升高,48 h達峰值,分彆為1.29 ±0.64和0.94±0.16,均顯著高于對照組的0.58 ±0.05和0.19±0.03(P值均＜0.05).ANP組大鼠胰腺組織ghrelin和GHSR蛋白的錶達在48 h時達峰值,分彆為3.05±0.48和2.34±0.32,均顯著高于對照組的2.18±0.23和1.55 ±0.10(P值均＜0.05).結論 ANP大鼠胰腺組織ghrelin、GHSR的mRNA及蛋白錶達均顯著增加,且與胰腺病變嚴重程度有關.
목적 관찰급성배사성이선염(ANP)대서이선조직ghrelin이급생장격소촉분비소수체(GHSR)적표체,탐토ghrelin재ANP발병궤제중적작용.방법 70지대서안완전수궤법분위ANP조화대조조,매조35지.채용역행담이관주사4％우광담산납적방법제비대서ANP모형；대조조부개복,경번동이선.술후3、6、12、24、48 h처사대서,취혈측혈청정분매,취이선행상규병리검사병평분,응용RTPCR화단백질인적법검측이선조직ghrelin、GHSR적mRNA급단백표체.결과 ANP조대서혈청정분매농도종조모후3h개시승고,6h체(8244±2950) U/L,현저고우대조조(P＜0.05)；이선병리손상급평분수시간연장축점가중,24h평분위(11.91 ±1.31)분,현저고우대조조적(3.12±1.60)분.ANP조대서이선조직ghrelin mRNA화GHSR mRNA표체수시간적연장이축점승고,48 h체봉치,분별위1.29 ±0.64화0.94±0.16,균현저고우대조조적0.58 ±0.05화0.19±0.03(P치균＜0.05).ANP조대서이선조직ghrelin화GHSR단백적표체재48 h시체봉치,분별위3.05±0.48화2.34±0.32,균현저고우대조조적2.18±0.23화1.55 ±0.10(P치균＜0.05).결론 ANP대서이선조직ghrelin、GHSR적mRNA급단백표체균현저증가,차여이선병변엄중정도유관.
Objective To observe the expression of ghrelin and growth hormone secretagogue receptor (GHSR) in pancreas of rats with acute necrotizing pancreatitis (ANP),and investigate the role of ghrelin in the pathogenesis of ANP.Methods Seventy SD rats were randomly divided into ANP group (n =35) and control group (n =35).ANP model was induced by retrograde injection of 4％ sodium taurocholate into the biliary and pancreatic duct.Rats in the control group underwent laparotomy with gentle pancreas manipulation only.At 3,6,12,24,48 h after ANP induction,the rats were sacrificed,and the serum level of amylase was determined,pathological changes in pancreatic tissue were routinely observed and scored.The expressions of ghrelin mRNA,protein and GHSR mRNA,protein in pancreas were evaluated by RT-PCR and Western blot.Results Serum amylase level began to increase at 3h after sodium taurocholate injection and reached the peak value at 6 h [(8244 ± 2950) U/L],which was significantly higher than that in control group (P ＜ 0.05).Pancreatic injuries was aggravated with time,the pathologic score at 24 h was (11.91 ± 1.31) score,which was significantly higher than (3.12 ± 1.60) score in the control group.The expressions of ghrelin mRNA and GHSR mRNA in pancreas of ANP group were increased gradually with time,and were significantly higher than those of control group at all time points,at 48 h,1.29 ±0.64 vs 0.58 ±0.05 and 0.94 ±0.16vs 0.19 ±0.03,P ＜ 0.05.The expressions of ghrelin protein and GHSR protein in pancreas of ANP group were significantly higher than that in control group at 12,24,48 h.at 48 h,3.05 ± 0.48 vs 2.18 ± 0.23 and 2.34 ± 0.32 vs 1.55 ± 0.10 (P ＜ 0.05).Conclusions The expressions of ghrelin mRNA,protein and GHSR mRNA,protein of pancreas are significantly increased in rats of ANP,and are associated with the severity of ANP.
