CAJ | 학술논문

目的观察microRNA-196a(miR-196a)抑制序列转染胰腺癌细胞株PANC1后对其HOXB8基因表达的影响.方法将PANC1细胞分为对照组、miR-196a抑制序列组和siRNA对照组.采用脂质体法将miR-196a抑制序列及对照siRNA分别转染PANCI细胞.应用RT-PCR和蛋白质印迹法检测转染细胞miR-196a及其下游靶基因HOXB8 mRNA和蛋白的表达.结果转染miR-196a抑制序列后,PANC1细胞miR-196a表达量较siRNA对照组显著减少(0.050±0.054比0.839±0.025,t=3.12,P＜0.05)；HOXB8 mRNA表达量较siRNA对照组增高1.57倍(2.20 ±0.07比1.29±0.10,t=3.86,P＜0.05)；HOXB8蛋白表达量也显著增强(0.90±0.03比0.40±0.10,t=3.11,P＜0.05).结论 miR-196a可以下调HOXB8基因的表达.
목적 관찰microRNA-196a(miR-196a)억제서렬전염이선암세포주PANC1후대기HOXB8기인표체적영향.방법 장PANC1세포분위대조조、miR-196a억제서렬조화siRNA대조조.채용지질체법장miR-196a억제서렬급대조siRNA분별전염PANCI세포.응용RT-PCR화단백질인적법검측전염세포miR-196a급기하유파기인HOXB8 mRNA화단백적표체.결과 전염miR-196a억제서렬후,PANC1세포miR-196a표체량교siRNA대조조현저감소(0.050±0.054비0.839±0.025,t=3.12,P＜0.05)；HOXB8 mRNA표체량교siRNA대조조증고1.57배(2.20 ±0.07비1.29±0.10,t=3.86,P＜0.05)；HOXB8단백표체량야현저증강(0.90±0.03비0.40±0.10,t=3.11,P＜0.05).결론 miR-196a가이하조HOXB8기인적표체.
Objective To investigate the effects of microRNA-196a(miR-196a) inhibitory sequences transfection on HOXB8 expression in PANC1 cells.Methods PANC1 cells were divided into control group,miR-196a inhibitory sequences group and siRNA control group.Liposomal transfection method was applied to transfect miR 196a inhibitory sequences and siRNA control into PANC1 cells.RT-PCR and Western blot were used to detect the expressions of miR-196a and HOXB8 mRNA and protein.Results After miR-196a inhibitory sequences transfection,when compared with that of siRNA control group,the expression of miR-196a was significantly decreased (0.05 ± 0.054 vs.0.839 ± 0.025,t =3.12,P ＜0.05) ; and the expression of HOXB8 mRNA was significantly increased by 1.57 folds (2.20 ± 0.07 vs.1.29 ± 0.10,t =3.86,P ＜ 0.05),the expression of HOXB8 protein was also obviously increased (0.90 ± 0.03 vs.0.40 ± 0.10,t =3.11,P ＜ 0.05).Conclusions MicroRNA-196a down-regulates the expression of HOXB8.