中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2013年
1期
32-35
,共4页
夏敏%张婷%郭继中%陈卫昌
夏敏%張婷%郭繼中%陳衛昌
하민%장정%곽계중%진위창
胰腺炎,急性坏死性%高迁移率族蛋白质类%抗体,单克隆
胰腺炎,急性壞死性%高遷移率族蛋白質類%抗體,單剋隆
이선염,급성배사성%고천이솔족단백질류%항체,단극륭
Pancreatits,acute necrotizing%High mobility group proteins%Antibodies,monoclonal
目的 探讨高迁移率族蛋白1(HMGB1)在急性坏死性胰腺炎(ANP)伴器官功能受损发生过程中的作用.方法 雄性ICR小鼠按数字表法随机分为对照组、ANP组和HMGB1单抗处理组(抗体组).采用20％L-精氨酸腹腔注射制备小鼠ANP模型,抗体组于建模成功后即刻给予腹腔内注射HMGB1单抗200 μg.制模后12、24、48 h分批处死小鼠,取血检测血清淀粉酶活性及肝、肾功能指标,ELASA法检测血HMGB1水平,取胰腺及肝脏组织行常规病理检查并评分,实时荧光定量PCR法检测胰腺和肝脏组织HMGB1 mRNA表达.结果 对照组、ANP组、抗体组12 h时的血清HMGB1水平分别为(9.09±1.03)、(25.04±4.30)、(16.84 ±4.27) μg/L；胰腺病理评分为(1.50±0.55)、(4.33±0.52)、(3.03±0.32)分；胰腺组织HMGB1 mRNA表达量为0.48 ±0.18、7.53 ±2.71、3.26 ±2.33;肝脏组织HMGB1 mRNA表达量为-1.23±0.37、0.15 ±0.65、-1.27 ±0.72.ANP组的上述指标均显著高于对照组(P值均＜0.05),而抗体组均显著低于ANP组(P值均＜0.05).造模后24 h血清HMGB1水平与胰腺病理评分呈线性正相关(r=0.768,P＜0.05).此外,各组血清淀粉酶、谷草转氨酶、谷丙转氨酶、乳酸脱氢酶、尿素氮、肌酐水平的变化趋势同血清HMGB1的变化,血清HMGB1水平与肌酐、尿素氮、谷丙转氨酶水平呈线性正相关(r值分别为0.824、0.719、0.590,p值均＜0.05).结论 HMGB1可能是ANP小鼠体内炎症反应和器官功能损害的关键因子,外源性给予其抗体可减轻ANP时胰腺及其他器官功能受损程度.
目的 探討高遷移率族蛋白1(HMGB1)在急性壞死性胰腺炎(ANP)伴器官功能受損髮生過程中的作用.方法 雄性ICR小鼠按數字錶法隨機分為對照組、ANP組和HMGB1單抗處理組(抗體組).採用20％L-精氨痠腹腔註射製備小鼠ANP模型,抗體組于建模成功後即刻給予腹腔內註射HMGB1單抗200 μg.製模後12、24、48 h分批處死小鼠,取血檢測血清澱粉酶活性及肝、腎功能指標,ELASA法檢測血HMGB1水平,取胰腺及肝髒組織行常規病理檢查併評分,實時熒光定量PCR法檢測胰腺和肝髒組織HMGB1 mRNA錶達.結果 對照組、ANP組、抗體組12 h時的血清HMGB1水平分彆為(9.09±1.03)、(25.04±4.30)、(16.84 ±4.27) μg/L；胰腺病理評分為(1.50±0.55)、(4.33±0.52)、(3.03±0.32)分；胰腺組織HMGB1 mRNA錶達量為0.48 ±0.18、7.53 ±2.71、3.26 ±2.33;肝髒組織HMGB1 mRNA錶達量為-1.23±0.37、0.15 ±0.65、-1.27 ±0.72.ANP組的上述指標均顯著高于對照組(P值均＜0.05),而抗體組均顯著低于ANP組(P值均＜0.05).造模後24 h血清HMGB1水平與胰腺病理評分呈線性正相關(r=0.768,P＜0.05).此外,各組血清澱粉酶、穀草轉氨酶、穀丙轉氨酶、乳痠脫氫酶、尿素氮、肌酐水平的變化趨勢同血清HMGB1的變化,血清HMGB1水平與肌酐、尿素氮、穀丙轉氨酶水平呈線性正相關(r值分彆為0.824、0.719、0.590,p值均＜0.05).結論 HMGB1可能是ANP小鼠體內炎癥反應和器官功能損害的關鍵因子,外源性給予其抗體可減輕ANP時胰腺及其他器官功能受損程度.
목적 탐토고천이솔족단백1(HMGB1)재급성배사성이선염(ANP)반기관공능수손발생과정중적작용.방법 웅성ICR소서안수자표법수궤분위대조조、ANP조화HMGB1단항처리조(항체조).채용20％L-정안산복강주사제비소서ANP모형,항체조우건모성공후즉각급여복강내주사HMGB1단항200 μg.제모후12、24、48 h분비처사소서,취혈검측혈청정분매활성급간、신공능지표,ELASA법검측혈HMGB1수평,취이선급간장조직행상규병리검사병평분,실시형광정량PCR법검측이선화간장조직HMGB1 mRNA표체.결과 대조조、ANP조、항체조12 h시적혈청HMGB1수평분별위(9.09±1.03)、(25.04±4.30)、(16.84 ±4.27) μg/L；이선병리평분위(1.50±0.55)、(4.33±0.52)、(3.03±0.32)분；이선조직HMGB1 mRNA표체량위0.48 ±0.18、7.53 ±2.71、3.26 ±2.33;간장조직HMGB1 mRNA표체량위-1.23±0.37、0.15 ±0.65、-1.27 ±0.72.ANP조적상술지표균현저고우대조조(P치균＜0.05),이항체조균현저저우ANP조(P치균＜0.05).조모후24 h혈청HMGB1수평여이선병리평분정선성정상관(r=0.768,P＜0.05).차외,각조혈청정분매、곡초전안매、곡병전안매、유산탈경매、뇨소담、기항수평적변화추세동혈청HMGB1적변화,혈청HMGB1수평여기항、뇨소담、곡병전안매수평정선성정상관(r치분별위0.824、0.719、0.590,p치균＜0.05).결론 HMGB1가능시ANP소서체내염증반응화기관공능손해적관건인자,외원성급여기항체가감경ANP시이선급기타기관공능수손정도.
Objective To investigate the role of HMGB1 in the pathogenesis of organ injury of acute necrotizing pancreatitis (ANP).Methods Male ICR mice were randomly allocated into control group,ANP group and HMGB1 monoclonal antibody group.ANP model was induced by intraperitoneal injection of 20％ L-arginine.Mice in HMGB1 monoclonal antibody group were given intraperitoneal injection of 200 μg of HMGB1 monoclonal antibody immediately after the induction of the ANP model.All the mice were sacrificed at 12,24,and 48 h after ANP induction.Serum level of amylase and liver,renal function were determined,level of serum HMGB1 was measured by using enzyme-linked immunosorbent assay,and then the pathologic changes of pancreas and liver were routinely observed and scored.The HMGB1 mRNA levels in the liver and pancreas were studied by real time fluorescence quantitative PCR.Results The serum levels of HMGB1 at 12 h in control group,ANP group and HMGB 1 monoclonal antibody group were (9.09 ± 1.03),(25.04 ± 4.30),(16.84 ± 4.27) μg/L; and pathological scores of pancreatic tissue were (1.50 ± 0.55),(4.33 ± 0.52),(3.03 ± 0.32) points ; and HMGB1 mRNA expressions in pancreas were 0.48 ± 0.18,7.53 ± 2.71,3.26 ±2.33 ; HMGB1 mRNA expressions in liver were-1.23 ± 0.37,0.15 ± 0.65,－ 1.27 ± 0.72.The corresponding values in ANP group were significantly higher than those in control group (P ＜ 0.05).While the corresponding values in HMGB1 monoclonal antibody group were significantly lower than those in ANP group (P ＜0.05).There was a positive linear relationship between serum HMGB1 level and pancreatic pathological scores 24 h after ANP induction (r =0.768,P ＜ 0.05).In addition,the serum levels of AMY,AST,ALT,LDH,BUN,Cr showed a similar trend as that of serum level of HMGB1,and the serum level of HMGB1 was positively associated with serum levels of Cr,BUN and ALT (r =0.824,0.719,0.590,P＜0.05).Conclusions HMGB1 may be a key factor of inflammatory response and organ dysfunction of ANP in mice,and extrinsic supply of its monoclonal antibody may decrease the injuries of pancreas and other organs during ANP.
