CAJ | 학술논문

目的评价不同浓度乙醇瘤内注射对裸鼠胰腺癌种植瘤的疗效,为临床选择合适的乙醇浓度治疗胰腺癌提供实验依据.方法将人胰腺癌SW1990细胞接种于裸鼠皮下建立胰腺癌种植瘤模型.选取肿瘤大小相近的裸鼠48只,按随机表法随机分为20％、40％、60％、80％、95％乙醇注射组及注射生理盐水的对照组.治疗前后用游标卡尺测量肿瘤最长径(a)和最短径(b),计算肿瘤体积(TV)、相对肿瘤体积(RTV)和相对肿瘤体积增殖率(T/C).治疗后第8天处死裸鼠,取肿瘤组织行病理组织学检查.结果 20％乙醇注射组与对照组的RTV差异无统计学意义(P=0.212),40％、60％、80％、95％乙醇注射组的RTV均较对照组显著缩小(P值均＜0.01),其中60％、80％、95％乙醇注射组的RTV＜l,T/C＜ 30％,且随着乙醇浓度的增加RTv及T/C值越小.80％和95％乙醇注射组的RTV值显著低于60％乙醇注射组(P值分别为0.003、0.009),而80％和95％乙醇注射组的RTV差异无统计学意义(P=0.819).病理组织学检查显示对照组无肿瘤坏死,20％、40％乙醇注射组种植瘤内可见少量坏死,60％、80％和95％乙醇注射组的种植瘤体内出现大面积凝固性坏死.结论选择80％乙醇瘤内注射治疗裸鼠皮下胰腺癌种植瘤是安全、有效的.
목적 평개불동농도을순류내주사대라서이선암충식류적료효,위림상선택합괄적을순농도치료이선암제공실험의거.방법 장인이선암SW1990세포접충우라서피하건립이선암충식류모형.선취종류대소상근적라서48지,안수궤표법수궤분위20％、40％、60％、80％、95％을순주사조급주사생리염수적대조조.치료전후용유표잡척측량종류최장경(a)화최단경(b),계산종류체적(TV)、상대종류체적(RTV)화상대종류체적증식솔(T/C).치료후제8천처사라서,취종류조직행병리조직학검사.결과 20％을순주사조여대조조적RTV차이무통계학의의(P=0.212),40％、60％、80％、95％을순주사조적RTV균교대조조현저축소(P치균＜0.01),기중60％、80％、95％을순주사조적RTV＜l,T/C＜ 30％,차수착을순농도적증가RTv급T/C치월소.80％화95％을순주사조적RTV치현저저우60％을순주사조(P치분별위0.003、0.009),이80％화95％을순주사조적RTV차이무통계학의의(P=0.819).병리조직학검사현시대조조무종류배사,20％、40％을순주사조충식류내가견소량배사,60％、80％화95％을순주사조적충식류체내출현대면적응고성배사.결론 선택80％을순류내주사치료라서피하이선암충식류시안전、유효적.
Objective To study the efficacy of intra-tumoral injection of different concentrations of ethanol for nude mice with implanted pancreatic cancer and provide evidence for choosing appropriate concentration of ethanol for clinical treatment of pancreatic cancer.Methods A subcutaneous xenograft mouse model of human pancreatic cancer SW1990 was established.Forty-eight nude mice with similar tumor size were randomly divided into 20％,40％,60％,80％,95％ ethanol injection groups and saline injection group.The longest (a) and the shortest diameters (b) of tumor of nude mice were measured.Tumor volume (TV),relative tumor volume (RTV) and the relative rate of tumor proliferation (T/C％) were calculated.Eight days later the nude mice were sacrificed.The tumor tissue was harvested for pathologic examinations.Results RTV in 20％ ethanol injection group was similar that of saline injection group (P =0.212).RTV in 40％,60％,80％ and 95％ ethanol injection groups were significantly lower than that in saline injection group (P ＜ 0.01).RTV was less than 1 and T/C％ was less than 30％ in 60％,80％ and 95％ ethanol injection groups.The values of RTV and T/C％ decreased with the increase of ethanol concentration.RTV in 80％ and 95％ ethanol injection groups were significantly lower than that of 60％ ethanol injection group (P =0.003 and P =0.009).RTV was similar in 80％ and 95％ ethanol injection groups (P =0.819).The pathologic examinations showed no tumor necrosis in saline injection group,while small amounts of necrosis in implanted pancreatic cancer was observed in 20％ and 40％ ethanol injection groups,while a large area of coagulation necrosis could be found in 60％,80％ and 95％ ethanol injection groups.Conclusions Intra-tumoral injection of 80％ ethanol is feasible therapy method for nude mice with human pancreatic cancer xenografts.