中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2013年
4期
240-243
,共4页
马丹%满晓华%高军%李兆申
馬丹%滿曉華%高軍%李兆申
마단%만효화%고군%리조신
胰腺肿瘤%免疫组织化学%Hedgehog信号通路%丝裂原激活蛋白激酶激酶类
胰腺腫瘤%免疫組織化學%Hedgehog信號通路%絲裂原激活蛋白激酶激酶類
이선종류%면역조직화학%Hedgehog신호통로%사렬원격활단백격매격매류
Pancreatic neoplasm%Immunohistochemistry%Hedgehog signal pathway%Mitogen-activated protein kinase kinases
目的 探讨Hedgehog信号通路重要成员Shh、Gli1、Sufu以及TAK1和磷酸化TAK1(p-TAK1)在胰腺癌组织中的表达及其与临床病理参数的相关性.方法 应用免疫组化法检测38例手术切除的胰腺癌组织及其配对的癌旁胰腺组织中Shh、Gli、Sufu、TAK1、p-TAK1蛋白的表达,分析它们与临床病理参数间的关系及它们相互间的关系.结果 胰腺癌组织Shh、Gli1、Sufu、TAK1、p-TAK1蛋白的表达率分别为86.8％(33/38)、52.6％(20/38)、68.4％(26/38)、55.3％(21/38)、52.6％(20/38),而癌旁胰腺组织中的表达均为阴性.Gli1表达与肿瘤远处转移及临床分期呈正相关(r值分别为0.524、0.361,P值均＜0.05)；Sufu表达与患者性别相关(r=-0.378,P＜0.05)；TAK1表达与胰腺癌临床分期呈正相关(r=0.468,P＜0.05)；p-TAK1表达与临床分期、肿瘤远处转移呈正相关(r值分别为0.418、0.361,P值均＜0.05).胰腺癌组织中Gli1的表达水平与TAK1及p-TAK1呈正相关(P＜0.05).结论 Hedgehog信号通路及TAK1途径在胰腺癌的发生、发展中具有一定作用,且两条途径可能存在一定的相互作用.
目的 探討Hedgehog信號通路重要成員Shh、Gli1、Sufu以及TAK1和燐痠化TAK1(p-TAK1)在胰腺癌組織中的錶達及其與臨床病理參數的相關性.方法 應用免疫組化法檢測38例手術切除的胰腺癌組織及其配對的癌徬胰腺組織中Shh、Gli、Sufu、TAK1、p-TAK1蛋白的錶達,分析它們與臨床病理參數間的關繫及它們相互間的關繫.結果 胰腺癌組織Shh、Gli1、Sufu、TAK1、p-TAK1蛋白的錶達率分彆為86.8％(33/38)、52.6％(20/38)、68.4％(26/38)、55.3％(21/38)、52.6％(20/38),而癌徬胰腺組織中的錶達均為陰性.Gli1錶達與腫瘤遠處轉移及臨床分期呈正相關(r值分彆為0.524、0.361,P值均＜0.05)；Sufu錶達與患者性彆相關(r=-0.378,P＜0.05)；TAK1錶達與胰腺癌臨床分期呈正相關(r=0.468,P＜0.05)；p-TAK1錶達與臨床分期、腫瘤遠處轉移呈正相關(r值分彆為0.418、0.361,P值均＜0.05).胰腺癌組織中Gli1的錶達水平與TAK1及p-TAK1呈正相關(P＜0.05).結論 Hedgehog信號通路及TAK1途徑在胰腺癌的髮生、髮展中具有一定作用,且兩條途徑可能存在一定的相互作用.
목적 탐토Hedgehog신호통로중요성원Shh、Gli1、Sufu이급TAK1화린산화TAK1(p-TAK1)재이선암조직중적표체급기여림상병리삼수적상관성.방법 응용면역조화법검측38례수술절제적이선암조직급기배대적암방이선조직중Shh、Gli、Sufu、TAK1、p-TAK1단백적표체,분석타문여림상병리삼수간적관계급타문상호간적관계.결과 이선암조직Shh、Gli1、Sufu、TAK1、p-TAK1단백적표체솔분별위86.8％(33/38)、52.6％(20/38)、68.4％(26/38)、55.3％(21/38)、52.6％(20/38),이암방이선조직중적표체균위음성.Gli1표체여종류원처전이급림상분기정정상관(r치분별위0.524、0.361,P치균＜0.05)；Sufu표체여환자성별상관(r=-0.378,P＜0.05)；TAK1표체여이선암림상분기정정상관(r=0.468,P＜0.05)；p-TAK1표체여림상분기、종류원처전이정정상관(r치분별위0.418、0.361,P치균＜0.05).이선암조직중Gli1적표체수평여TAK1급p-TAK1정정상관(P＜0.05).결론 Hedgehog신호통로급TAK1도경재이선암적발생、발전중구유일정작용,차량조도경가능존재일정적상호작용.
Objective To investigate the expression of Hedgehog signaling pathway members,Shh,Gli1,Sufu and TAK1 as well as phosphorylation-TAK1 (p-TAK1) protein in human pancreatic carcinoma tissues and to explore its relationship between the expression of proteins and the clinicopathologic parameters.Methods The expressions of Shh,Glil,Sufu,TAK1,p-TAK1 proteins in 38 samples of pancreatic cancer tissues and pairing adjacent normal pancreatic tissues were detected by immunohistochemical method.The relationship among the proteins and the relationship between the expression of the proteins and the clinicopathologic parameters were examined.Results The positive expression rates of Shh,Glil,Sufu,TAK1,p-TAK1 protein in human pancreatic carcinoma were 86.8％ (33/38),52.6％ (22/38),68.4％(26/38),55.3％ (21/38),52.6％ (20/38),but they were not expressed in adjacent normal pancreatic tissues.Gli1 expression was positively related to distant metastasis and clinical staging (r =0.524,0.361,P ＜0.05),Sufu expression was positively related to patient's gender (r =-0.378,P ＜0.05),TAK1 expression was positively related to clinical staging (r =0.468,P ＜ 0.05),p-TAK1 expression was positively related to clinical staging and distant metastasis (r =0.418,0.361,P ＜ 0.05).Gli1 expression was positively related to TAK1 and p-TAK1 expression in pancreatic cancer tissues (P ＜ 0.05).Conclusions Hedgehog signaling pathway and p-TAK1 may play a role in the pathogenesis of pancreatic cancer,and the two pathways may interact with each other.
