CAJ | 학술논문

目的探讨不同剂量砷暴露大鼠唾液中含砷量变化及其与血砷、尿砷间的关系.方法将32只雄性SD大鼠按体质量随机分为4组,分别为对照组(生理盐水)及低(0.2 mg/kg)、中(2.0 mg/kg)、高(20.0mg/kg)剂量亚砷酸钠染毒组,每组8只.采用经口灌胃染毒方法,隔日1次,连续2周后收集唾液、血液、尿液及脏器,计算脏器系数,应用原子荧光分光光度计(AFS-230)检测血砷、尿砷,应用电感耦合-等离子体质谱(ICP-MS)检测唾液砷.结果亚砷酸钠染毒组大鼠体质量增长值均低于对照组,其中高剂量组[(76.13±17.19)g]与对照组[(103.00±12.31)g]比较,差异有统计学意义(P＜0.05).高剂量组大鼠肝脏及肾脏脏器系数[(3.92±0.54)％、(0.96±0.15)％]与对照组[(3.27±0.35)％、(0.76±0.05)％]比较,差异有统计学意义(P＜0.05或＜0.01).低、中、高染毒组大鼠唾液砷[(0.044±0.019)、(0.211±0.071)、(1.128±0.380)mg/L]、血砷[(11.832±1.887)、(45.032±7.216)、(121.839±17.323)mg/L]及尿砷[(0.138±0.085)、(0.874±0.328)、(8.843±1.754)mg/L]与对照组[(0.018±0.014)、(2.267±0.370)、(0.025±0.011)mg/L]比较,差异有统计学意义(P均＜ 0.05),且各染毒组之间比较差异有统计学意义(P均＜0.05).大鼠唾液砷与血砷、尿砷之间存在显著正相关,相关系数分别为0.934、0.960(P均＜0.01).结论高砷暴露可抑制大鼠体质量增长,并对肝脏及肾脏有较强毒性.砷在唾液中存在良好的剂量-反应关系,且唾液砷与血砷、尿砷间存在明显的正相关关系,提示唾液砷也是一种良好的砷暴露标志物.
목적 탐토불동제량신폭로대서타액중함신량변화급기여혈신、뇨신간적관계.방법 장32지웅성SD대서안체질량수궤분위4조,분별위대조조(생리염수)급저(0.2 mg/kg)、중(2.0 mg/kg)、고(20.0mg/kg)제량아신산납염독조,매조8지.채용경구관위염독방법,격일1차,련속2주후수집타액、혈액、뇨액급장기,계산장기계수,응용원자형광분광광도계(AFS-230)검측혈신、뇨신,응용전감우합-등리자체질보(ICP-MS)검측타액신.결과 아신산납염독조대서체질량증장치균저우대조조,기중고제량조[(76.13±17.19)g]여대조조[(103.00±12.31)g]비교,차이유통계학의의(P＜0.05).고제량조대서간장급신장장기계수[(3.92±0.54)％、(0.96±0.15)％]여대조조[(3.27±0.35)％、(0.76±0.05)％]비교,차이유통계학의의(P＜0.05혹＜0.01).저、중、고염독조대서타액신[(0.044±0.019)、(0.211±0.071)、(1.128±0.380)mg/L]、혈신[(11.832±1.887)、(45.032±7.216)、(121.839±17.323)mg/L]급뇨신[(0.138±0.085)、(0.874±0.328)、(8.843±1.754)mg/L]여대조조[(0.018±0.014)、(2.267±0.370)、(0.025±0.011)mg/L]비교,차이유통계학의의(P균＜ 0.05),차각염독조지간비교차이유통계학의의(P균＜0.05).대서타액신여혈신、뇨신지간존재현저정상관,상관계수분별위0.934、0.960(P균＜0.01).결론 고신폭로가억제대서체질량증장,병대간장급신장유교강독성.신재타액중존재량호적제량-반응관계,차타액신여혈신、뇨신간존재명현적정상관관계,제시타액신야시일충량호적신폭로표지물.
Objective To investigate the relations between saliva arsenic levels and serum arsenic and urinary arsenic of rats after exposed to different levels of sodium arsenite.Methods Thirty-two SD rats were randomly divided into four groups(8 rats in each group),namely the control group,the low,the medium,and the high doses of sodium arsenite exposure groups.Rats of the control group were given 0.9％ NaCI by gavage,and other three groups were given sodium arsenite of 0.2,2.0,20.0 mg/kg body weight by gavage.All animals were administrated every other day for two weeks,then saliva,blood,urine and tissue organs were collected,organ coefficients were calculated,total arsenic concentrations in blood and urine were detected by Atomic Fluorescence Spectrometry(AFS-230) and total arsenic concentration in saliva was detected by Inductively Coupled Plasma Mass Spectrometer(ICP-MS).Results The weight gain values of rats exposed to sodium arsenite were lower than that of the control group,the difference was statistically significant between the highest dose group[(76.13 ± 17.19)g]and the control group[(103.00 ± 12.31)g,P ＜ 0.05].The liver and kidney organ coefficients in the highest dose group [(3.92 ± 0.54)％,(0.96 ± 0.15)％]were significantly higher than that in the control group[(3.27 ± 0.35)％,(0.76 ± 0.05)％,P ＜ 0.05 or ＜ 0.01].The total arsenic concentrations in saliva[(0.044 ± 0.019),(0.211 ± 0.071),(1.128 ± 0.380)mg/L],blood[(11.832 ± 1.887),(45.032 ± 7.216),(121.839 ± 17.323)mg/L]and urine[(0.138 ± 0.085),(0.874 ± 0.328),(8.843 ± 1.754)mg/L]in the three treatment groups were significantly higher compared with that of the control group [(0.018 ± 0.014),(2.267 ± 0.370),(0.025 ± 0.011)mg/L,all P ＜ 0.05],furthermore,there was a significant difference among the three treatment groups (all P ＜ 0.05).The arsenic contents in saliva were significantly correlated with blood arsenic and urinary arsenic,the correlation coefficient was 0.934 and 0.960,respectively (all P ＜ 0.01).Conclusions High dose of arsenic exposure,with a strong toxicity to liver and kidney,can inhibit the increase of rat body weight.Arsenic dose-response relationship exists in the saliva,and saliva arsenic is significantly correlated with blood arsenic and urinary arsenic,suggesting that salivary arsenic can be used as a new biomarker for assessing human exposure to arsenic.