中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2012年
6期
640-642
,共3页
席进孝%张宏%格鹏飞%郭丽民%达文平%吴斌%徐大琴%王世明%潘卫民%苗克军%葛亚俊%穆洮霞%陈国娟
席進孝%張宏%格鵬飛%郭麗民%達文平%吳斌%徐大琴%王世明%潘衛民%苗剋軍%葛亞俊%穆洮霞%陳國娟
석진효%장굉%격붕비%곽려민%체문평%오빈%서대금%왕세명%반위민%묘극군%갈아준%목조하%진국연
鼠疫%受试者工作特征曲线%上转换发光技术%诊断%评价研究
鼠疫%受試者工作特徵麯線%上轉換髮光技術%診斷%評價研究
서역%수시자공작특정곡선%상전환발광기술%진단%평개연구
Plague%receiver operating characteristic curve%Up-conversion technology%Diagnosis%Evaluation studies
目的 用受试者工作特征曲线(receiver operating characteristic curve,简称ROC曲线)法评价上转发光技术(UPT)快速检测鼠疫抗原抗体的效果,为UPT快速检测技术的鼠疫现场应用提供依据.方法 在喜马拉雅旱獭和阿拉善黄鼠疫源地采集动物血清标本224份,用UPT、酶联免疫吸附试验(ELISA)、金标、间接血凝试验(IHA)4种方法检测鼠疫菌F1抗体；采集旱獭、黄鼠脏器、骨髓标本108份,用UPT、ELISA、金标、PCR、反向间接血凝试验(RIHA)5种方法检测鼠疫菌F1抗原.抗体检测结果以IHA为标准,抗原检测结果分别以RIHA 、PCR+金标、PCR+ ELISA为标准,结果应用ROC法进行评价.结果 抗体检测UPT、ELISA、金标3种方法的曲线下面积(AUC)均＞0.5,UPT的AUC与其他方法比较,差异无统计学意义(z=1.204,P＞ 0.05).抗原检测UPT、ELISA、金标、PCR 4种方法AUC均＞0.5,UPT的AUC与其他方法比较,差异无统计学意义(z=0.866,P＞ 0.05).结论 UPT快速检测技术对鼠疫抗原抗体检测效果良好,该技术操作简单,快速,结果准确,适合鼠疫现场监测、突发鼠疫疫情应急处理,适宜推广应用.
目的 用受試者工作特徵麯線(receiver operating characteristic curve,簡稱ROC麯線)法評價上轉髮光技術(UPT)快速檢測鼠疫抗原抗體的效果,為UPT快速檢測技術的鼠疫現場應用提供依據.方法 在喜馬拉雅旱獺和阿拉善黃鼠疫源地採集動物血清標本224份,用UPT、酶聯免疫吸附試驗(ELISA)、金標、間接血凝試驗(IHA)4種方法檢測鼠疫菌F1抗體；採集旱獺、黃鼠髒器、骨髓標本108份,用UPT、ELISA、金標、PCR、反嚮間接血凝試驗(RIHA)5種方法檢測鼠疫菌F1抗原.抗體檢測結果以IHA為標準,抗原檢測結果分彆以RIHA 、PCR+金標、PCR+ ELISA為標準,結果應用ROC法進行評價.結果 抗體檢測UPT、ELISA、金標3種方法的麯線下麵積(AUC)均＞0.5,UPT的AUC與其他方法比較,差異無統計學意義(z=1.204,P＞ 0.05).抗原檢測UPT、ELISA、金標、PCR 4種方法AUC均＞0.5,UPT的AUC與其他方法比較,差異無統計學意義(z=0.866,P＞ 0.05).結論 UPT快速檢測技術對鼠疫抗原抗體檢測效果良好,該技術操作簡單,快速,結果準確,適閤鼠疫現場鑑測、突髮鼠疫疫情應急處理,適宜推廣應用.
목적 용수시자공작특정곡선(receiver operating characteristic curve,간칭ROC곡선)법평개상전발광기술(UPT)쾌속검측서역항원항체적효과,위UPT쾌속검측기술적서역현장응용제공의거.방법 재희마랍아한달화아랍선황서역원지채집동물혈청표본224빈,용UPT、매련면역흡부시험(ELISA)、금표、간접혈응시험(IHA)4충방법검측서역균F1항체；채집한달、황서장기、골수표본108빈,용UPT、ELISA、금표、PCR、반향간접혈응시험(RIHA)5충방법검측서역균F1항원.항체검측결과이IHA위표준,항원검측결과분별이RIHA 、PCR+금표、PCR+ ELISA위표준,결과응용ROC법진행평개.결과 항체검측UPT、ELISA、금표3충방법적곡선하면적(AUC)균＞0.5,UPT적AUC여기타방법비교,차이무통계학의의(z=1.204,P＞ 0.05).항원검측UPT、ELISA、금표、PCR 4충방법AUC균＞0.5,UPT적AUC여기타방법비교,차이무통계학의의(z=0.866,P＞ 0.05).결론 UPT쾌속검측기술대서역항원항체검측효과량호,해기술조작간단,쾌속,결과준학,괄합서역현장감측、돌발서역역정응급처리,괄의추엄응용.
Objective To evaluate the effect of up-converting phosphor technology(UPT) in detection of plague antigen-antibody by receiver operating characteristic curve (ROC) method,and to provide a scientific basis for field application of UPT rapid detection technology in plague prevention and control.Methods Two hundred and twenty four serum samples were collected from Marmots and ground squirrels in the plague foci,Yersinia pestis antibody was detected by UPT,ELISA,Colloidal-gold Strips and IHA,respectively; 108 organs and bone marrow samples were collected,and Yersinia pestis antigens were detected by UPT,ELISA,PCR and RIHA,respectively.IHA was used as the gold standard for antibody test results,RIHA,PCR + Colloidal-gold Strips,PCR + ELISA were used as the gold standard for antigen test results.The results were evaluated using ROC method.Results Antibodies detection:the AUCs of UPT,ELISA and Colloidal-gold Strips were greater than 0.5.The difference between UPT and other methods was not statistically significant (z =1.204,P ＞ 0.05).Antigen detection:the AUCs of UPT,ELISA,Colloidal-gold Strips and PCR were greater than 0.5.There was no statistical difference between UPT and other methods(z =0.866,P ＞ 0.05).Conclusions UPT as a new technology works well in the detection of plague antigen-antibody.The technology is simple,fast,accurate,and suitable for on-site monitoring of plague,emergency treatment of sudden plague,and suitable for promotion.
