中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2012年
6期
681-683
,共3页
王信惠%徐恩洁%雷刚%廖力夫%热娜·吐尔地%涂杰%阿不力米提·买托呼提%李冰%徐秉臣
王信惠%徐恩潔%雷剛%廖力伕%熱娜·吐爾地%塗傑%阿不力米提·買託呼提%李冰%徐秉臣
왕신혜%서은길%뢰강%료력부%열나·토이지%도걸%아불력미제·매탁호제%리빙%서병신
鼠疫%免疫球蛋白M%酶联免疫吸附测定
鼠疫%免疫毬蛋白M%酶聯免疫吸附測定
서역%면역구단백M%매련면역흡부측정
Plague%Immunoglobulin M%Enzyme-linked immunosorbent assay
目的 建立酶联免疫吸附试验(ELISA)捕获法,检测牧犬血清中鼠疫IgM抗体.方法 用抗犬血清IgM抗体包被反应板,捕获牧犬血清样本中的IgM,并用ELISA夹心法同步检测牧犬血清中鼠疫F1抗体.结果 共检测牧犬血清216份,其中ELISA夹心法检出鼠疫F1抗体阳性26份,阳性率为12.03％(26/216),ELISA捕获法检出IgM抗体阳性14份,阳性率为6.48％(14/216),占总阳性的53.85％(14/26).结论 ELISA 捕获法检测牧犬鼠疫IgM抗体,可间接推算鼠疫疫源地近期动物鼠疫流行时间和分布,为及时实施防控措施提供参考资料.
目的 建立酶聯免疫吸附試驗(ELISA)捕穫法,檢測牧犬血清中鼠疫IgM抗體.方法 用抗犬血清IgM抗體包被反應闆,捕穫牧犬血清樣本中的IgM,併用ELISA夾心法同步檢測牧犬血清中鼠疫F1抗體.結果 共檢測牧犬血清216份,其中ELISA夾心法檢齣鼠疫F1抗體暘性26份,暘性率為12.03％(26/216),ELISA捕穫法檢齣IgM抗體暘性14份,暘性率為6.48％(14/216),佔總暘性的53.85％(14/26).結論 ELISA 捕穫法檢測牧犬鼠疫IgM抗體,可間接推算鼠疫疫源地近期動物鼠疫流行時間和分佈,為及時實施防控措施提供參攷資料.
목적 건립매련면역흡부시험(ELISA)포획법,검측목견혈청중서역IgM항체.방법 용항견혈청IgM항체포피반응판,포획목견혈청양본중적IgM,병용ELISA협심법동보검측목견혈청중서역F1항체.결과 공검측목견혈청216빈,기중ELISA협심법검출서역F1항체양성26빈,양성솔위12.03％(26/216),ELISA포획법검출IgM항체양성14빈,양성솔위6.48％(14/216),점총양성적53.85％(14/26).결론 ELISA 포획법검측목견서역IgM항체,가간접추산서역역원지근기동물서역류행시간화분포,위급시실시방공조시제공삼고자료.
Objective To establish a capture enzyme-linked immunosorbent assay (ELISA) for detection of plague IgM antibody in herding dogs.Methods ELISA plates were coated with serum IgM antibody against dogs and F1 antibody to plague in the serum of herding dogs was detected by a sandwich ELISA.Results A total of 216 serum samples of herding dogs were tested,26 were positive for plague F1 antibody and the positive rate was 12.03％ (26/216); 14 were positive for plague IgM antibody and the positive rate was 6.48％ (14/216); IgM positive accounted for 53.8％(14/26) of all positive samples.Conclusions Serum plague IgM antibody of herding dogs can be used to predict the prevalent time and distribution of recent animal plague in plague foci indirectly,and to provide reference information for timely implementation of control measures.
