中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2013年
1期
13-15
,共3页
亚砷酸盐类%黑色素瘤%一元酚单氧酶
亞砷痠鹽類%黑色素瘤%一元酚單氧酶
아신산염류%흑색소류%일원분단양매
Arsenite%Melanoma%Monophenol monooxygenase
目的 探讨亚砷酸钠(NaAsO2)暴露对黑色素瘤细胞A375(以下简称A375)和G361(以下简称G361)的色素水平及酪氨酸酶(TYR)活力的影响,以及两种细胞色素代谢能力的差异.方法 以0.0(对照)、0.1、1.0 μmol/L NaAsO2作用于A375和G361细胞,72 h后,以阿尔玛蓝还原法检测细胞活力水平,同时测定细胞中黑色素水平及TYR的活力水平.结果 NaAsO2作用72h后,0.1 μmol/L剂量组的A375和G361细胞均呈轻度增殖表现,细胞活力[A375:(103.32±1.26)％；G361:(104.10±1.76)％]与对照组[A375:(100.00±1.08)％；G361:(100.00±1.79)％]比较,差异无统计学意义(P均＞0.05)；1.0μmol/L剂量组细胞活力[A375:(75.32±1.59)％；G361:(78.26±2.10)％]与对照组相比均显著下降(P均＜0.05).对照、0.1、1.0μmol/L剂量组的G361细胞的黑色素水平[(7.19±0.35)、(7.34±0.83)、(8.19±0.86) pg/细胞]均高于A375细胞[(4.35±0.72)、(4.54±0.01)、(4.60±0.59)pg/细胞,P均＜0.05],G361细胞的TYR活力水平[(54.13±1.21)、(54.56±0.21)、(56.25±0.85)贝克勒尔(Bq)]均高于A375细胞[(42.00±0.21)、(42.90±0.54)、(42.91±0.01)Bq,P均＜0.05].2种细胞的黑色素生成水平及TYR活力水平均随NaAsO2剂量增高而呈增高趋势,但组间比较,差异均无统计学意义(P均＞ 0.05).结论 砷致色素代谢异常可能与砷暴露致黑色素水平及TYR活力增高有关；不同个体间色素代谢差异,可能与个体的黑色素代谢本底水平及TYR本底活力差异有关.
目的 探討亞砷痠鈉(NaAsO2)暴露對黑色素瘤細胞A375(以下簡稱A375)和G361(以下簡稱G361)的色素水平及酪氨痠酶(TYR)活力的影響,以及兩種細胞色素代謝能力的差異.方法 以0.0(對照)、0.1、1.0 μmol/L NaAsO2作用于A375和G361細胞,72 h後,以阿爾瑪藍還原法檢測細胞活力水平,同時測定細胞中黑色素水平及TYR的活力水平.結果 NaAsO2作用72h後,0.1 μmol/L劑量組的A375和G361細胞均呈輕度增殖錶現,細胞活力[A375:(103.32±1.26)％；G361:(104.10±1.76)％]與對照組[A375:(100.00±1.08)％；G361:(100.00±1.79)％]比較,差異無統計學意義(P均＞0.05)；1.0μmol/L劑量組細胞活力[A375:(75.32±1.59)％；G361:(78.26±2.10)％]與對照組相比均顯著下降(P均＜0.05).對照、0.1、1.0μmol/L劑量組的G361細胞的黑色素水平[(7.19±0.35)、(7.34±0.83)、(8.19±0.86) pg/細胞]均高于A375細胞[(4.35±0.72)、(4.54±0.01)、(4.60±0.59)pg/細胞,P均＜0.05],G361細胞的TYR活力水平[(54.13±1.21)、(54.56±0.21)、(56.25±0.85)貝剋勒爾(Bq)]均高于A375細胞[(42.00±0.21)、(42.90±0.54)、(42.91±0.01)Bq,P均＜0.05].2種細胞的黑色素生成水平及TYR活力水平均隨NaAsO2劑量增高而呈增高趨勢,但組間比較,差異均無統計學意義(P均＞ 0.05).結論 砷緻色素代謝異常可能與砷暴露緻黑色素水平及TYR活力增高有關；不同箇體間色素代謝差異,可能與箇體的黑色素代謝本底水平及TYR本底活力差異有關.
목적 탐토아신산납(NaAsO2)폭로대흑색소류세포A375(이하간칭A375)화G361(이하간칭G361)적색소수평급락안산매(TYR)활력적영향,이급량충세포색소대사능력적차이.방법 이0.0(대조)、0.1、1.0 μmol/L NaAsO2작용우A375화G361세포,72 h후,이아이마람환원법검측세포활력수평,동시측정세포중흑색소수평급TYR적활력수평.결과 NaAsO2작용72h후,0.1 μmol/L제량조적A375화G361세포균정경도증식표현,세포활력[A375:(103.32±1.26)％；G361:(104.10±1.76)％]여대조조[A375:(100.00±1.08)％；G361:(100.00±1.79)％]비교,차이무통계학의의(P균＞0.05)；1.0μmol/L제량조세포활력[A375:(75.32±1.59)％；G361:(78.26±2.10)％]여대조조상비균현저하강(P균＜0.05).대조、0.1、1.0μmol/L제량조적G361세포적흑색소수평[(7.19±0.35)、(7.34±0.83)、(8.19±0.86) pg/세포]균고우A375세포[(4.35±0.72)、(4.54±0.01)、(4.60±0.59)pg/세포,P균＜0.05],G361세포적TYR활력수평[(54.13±1.21)、(54.56±0.21)、(56.25±0.85)패극륵이(Bq)]균고우A375세포[(42.00±0.21)、(42.90±0.54)、(42.91±0.01)Bq,P균＜0.05].2충세포적흑색소생성수평급TYR활력수평균수NaAsO2제량증고이정증고추세,단조간비교,차이균무통계학의의(P균＞ 0.05).결론 신치색소대사이상가능여신폭로치흑색소수평급TYR활력증고유관；불동개체간색소대사차이,가능여개체적흑색소대사본저수평급TYR본저활력차이유관.
Objective To study the impact of sodium arsenite(NaAsO2) exposure on melanoma cells A375 (hereinafter referred to as the A375) and G361 (hereinafter referred to as the G361) pigment production and tyrosinase (TYR) activity and the differences of pigment metabolism capacity between the cell lines.Methods A375 and G361 cells were exposed to sodium arsenite at concentrations of 0.0(control),0.1 and 1.0 μmol/L for 72hours.Cell viability was measured by Alamar Blue assay.Melanin levels and TYR activity were measured at the same time.Results After exposure for 72 hours,the cells of 0.1 μmol/L dose groups of both of the two cell lines [A375:(103.32 + 1.26)％; G361:(104.10 + 1.76)％] showed a slightincrease of proliferation without significant differences compared with those of the control[A375:(100.00 ± 1.08)％; G361:(100.00 + 1.79)％,all P ＜ 0.05] ;while cell viability of the 1.0 μmol/L dose group of both of the two cell lines[A375:(75.32 ± 1.59)％; G361:(78.26 ± 2.10)％] were significantly lower than those of the control (all P ＜ 0.05).Melanin levels of G361 cell line [(7.19 ± 0.35),(7.34 ± 0.83),(8.19 ± 0.86)pg/cell] were significantly higher than that of A375[(4.35 ± 0.72),(4.54 ± 0.01),(4.60 + 0.59)pg/cell,all P ＜ 0.05] in all the three groups.TYR activity of G361 cell line [(54.13 ± 1.21),(54.56 ± 0.21),(56.25 ± 0.85)Bq] were also markedly higher than that of A375 cell[(42.00 ±0.21),(42.90 ± 0.54),(42.91 ± 0.01)Bq,all P ＜ 0.05] in all the three groups.The melanin levels and TYR activities of both of the two cells lines showed an increase tendency along with increased doses of arsenic exposure,but without significant differences when compared with those of the three groups (all P ＞ 0.05).Conclusions Arsenic related pigment disorder may be associated with increased melanin levels and TYR activities induced by arsenic exposure; individual difference of pigment metabolism may be associated with different basal melanin levels and TYR activity between different individuals.
