CAJ | 학술논문

目的观察慢性砷暴露对雌性大鼠心肌雌激素受体结合位点相关抗原9(Ebag9)和雌激素反应指蛋白(efp) mRNA表达的影响.方法将50只雌性Wistar大鼠按体质量随机分成5组,每组10只,分别饮用含砷(三氧化二砷)量为0.00(对照)、0.05、0.10、0.20、0.40 mg/L的水溶液,32周后通过实时定量PCR方法检测大鼠心肌中Ebag9和efp mRNA的表达.结果 0.00、0.05、0.10、0.20、0.40 mg/L组大鼠心肌Ebag9、efpmRNA表达量分别为0.54±0.14、0.52±0.10、0.48±0.24、0.58±0.13、0.45±0.19和0.85±0.14、0.86±0.12、0.87±0.09、0.99±0.10、0.86±0.19.与对照组比较,0.20 mg/L组Ebag9 mRNA表达有升高趋势,而其他各染砷组有降低趋势,但差异均无统计学意义(P均＞ 0.05).与对照组比较,0.20 mg/L组efp mRNA表达明显升高(P＜0.05),而其他各染砷组有升高趋势,但差异均无统计学意义(P均＞ 0.05).结论慢性砷暴露可引起雌性大鼠心肌Ebag9和efp mRNA表达改变.砷可能对雌性大鼠心肌存在内分泌干扰效应.
목적 관찰만성신폭로대자성대서심기자격소수체결합위점상관항원9(Ebag9)화자격소반응지단백(efp) mRNA표체적영향.방법 장50지자성Wistar대서안체질량수궤분성5조,매조10지,분별음용함신(삼양화이신)량위0.00(대조)、0.05、0.10、0.20、0.40 mg/L적수용액,32주후통과실시정량PCR방법검측대서심기중Ebag9화efp mRNA적표체.결과 0.00、0.05、0.10、0.20、0.40 mg/L조대서심기Ebag9、efpmRNA표체량분별위0.54±0.14、0.52±0.10、0.48±0.24、0.58±0.13、0.45±0.19화0.85±0.14、0.86±0.12、0.87±0.09、0.99±0.10、0.86±0.19.여대조조비교,0.20 mg/L조Ebag9 mRNA표체유승고추세,이기타각염신조유강저추세,단차이균무통계학의의(P균＞ 0.05).여대조조비교,0.20 mg/L조efp mRNA표체명현승고(P＜0.05),이기타각염신조유승고추세,단차이균무통계학의의(P균＞ 0.05).결론 만성신폭로가인기자성대서심기Ebag9화efp mRNA표체개변.신가능대자성대서심기존재내분비간우효응.
Objective To observe the effects of chronic arsenic exposure on estrogen receptor-binding fragment-associated gene 9 (Ebag9) and estrogen-responsive finger protein (efp) mRNA expression in female rat' s myocardium.Methods Fifty female Wistar rats were randomly divided into five groups according to arsenic (As2O3) concentrations in drinking-water:0.00(control),0.05,0.10,0.20,0.40 mg/L groups and RT-PCR was used to detect Ebag9 and efp mRNA expression of myocardium at the 32 weeks of experiment.Results Ebag9 and efp mRNA expression levels in 0.00,0.05,0.10,0.20,0.40 mg/L groups were respectively as follows:0.54 ±0.14,0.52 ± 0.10,0.48 ± 0.24,0.58 ± 0.13,0.45 ± 0.19 and 0.85 ± 0.14,0.86 ± 0.12,0.87 ± 0.09,0.99 ±0.10,0.86 ± 0.19.Compared to the control group,Ebag9 mRNA level of the 0.20 mg/L group was increased,and decreased in other groups,but the difference between two groups was not significant(all P ＞ 0.05).Compared to control group,the efp mRNA level of 0.20 mg/L group increased significantly(P ＜ 0.05),and showed increased tendency in other arsenic groups,but the difference between two groups was not significant (all P ＞ 0.05).Conclusions Ebag9 and efp mRNA expression have changed in myocardium of rats exposed to chronic arsenic.Arsenic may has endocrine disruptor effect to female rat's myocardium.