中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2013年
1期
46-49
,共4页
刘丽香%叶永祥%禹雪%纪晓红%于钧%张智毅%刘守军%申红梅%刘鹏
劉麗香%葉永祥%禹雪%紀曉紅%于鈞%張智毅%劉守軍%申紅梅%劉鵬
류려향%협영상%우설%기효홍%우균%장지의%류수군%신홍매%류붕
酪氨酸%甲状腺球蛋白%碘化物过氧化物酶%细胞培养技术
酪氨痠%甲狀腺毬蛋白%碘化物過氧化物酶%細胞培養技術
락안산%갑상선구단백%전화물과양화물매%세포배양기술
Tyrosine%Thyroglobulin%Iodine peroxidase%Cell culture techniques
目的 研究酪氨酸对体外培养大鼠甲状腺细胞甲状腺球蛋白(TG)、甲状腺过氧化物酶(TPO)、钠碘转运体(NIS)及促甲状腺素受体(TSHR)mRNA表达的影响.方法 将体外培养大鼠甲状腺细胞分为对照组、酪氨酸Ⅰ组、酪氨酸Ⅱ组,每组5例,分别用含0、5%、10%酪氨酸的培养液培养96 h,收集甲状腺细胞,应用实时荧光定量PCR检测TG、TPO、NIS、TSHR mRNA表达.结果 对照组、酪氨酸Ⅰ组、酪氨酸Ⅱ组TG、NIS mRNA表达分别为1.11±0.36、2.08±0.45、2.52±0.65和1.21±0.36、1.94±0.57、2.10±0.56,组间比较差异有统计学意义(F值分别为10.48、4.42,P均<0.05),其中酪氨酸Ⅰ组、酪氨酸Ⅱ组TG、NIS mRNA表达明显高于对照组(P均< 0.05).上述3组TPO、TSHR mRNA表达分别为1.02±0.54、1.14±0.29、1.17±0.38和0.81±0.44、0.91±0.30、0.90±0.19,组间比较差异无统计学意义(F值分别为0.18、0.26,P均>0.05).结论 酪氨酸可能影响甲状腺细胞功能相关基因表达,增加碘在甲状腺的储存,通过作用于碘泵,调节甲状腺细胞的碘摄取功能.
目的 研究酪氨痠對體外培養大鼠甲狀腺細胞甲狀腺毬蛋白(TG)、甲狀腺過氧化物酶(TPO)、鈉碘轉運體(NIS)及促甲狀腺素受體(TSHR)mRNA錶達的影響.方法 將體外培養大鼠甲狀腺細胞分為對照組、酪氨痠Ⅰ組、酪氨痠Ⅱ組,每組5例,分彆用含0、5%、10%酪氨痠的培養液培養96 h,收集甲狀腺細胞,應用實時熒光定量PCR檢測TG、TPO、NIS、TSHR mRNA錶達.結果 對照組、酪氨痠Ⅰ組、酪氨痠Ⅱ組TG、NIS mRNA錶達分彆為1.11±0.36、2.08±0.45、2.52±0.65和1.21±0.36、1.94±0.57、2.10±0.56,組間比較差異有統計學意義(F值分彆為10.48、4.42,P均<0.05),其中酪氨痠Ⅰ組、酪氨痠Ⅱ組TG、NIS mRNA錶達明顯高于對照組(P均< 0.05).上述3組TPO、TSHR mRNA錶達分彆為1.02±0.54、1.14±0.29、1.17±0.38和0.81±0.44、0.91±0.30、0.90±0.19,組間比較差異無統計學意義(F值分彆為0.18、0.26,P均>0.05).結論 酪氨痠可能影響甲狀腺細胞功能相關基因錶達,增加碘在甲狀腺的儲存,通過作用于碘泵,調節甲狀腺細胞的碘攝取功能.
목적 연구락안산대체외배양대서갑상선세포갑상선구단백(TG)、갑상선과양화물매(TPO)、납전전운체(NIS)급촉갑상선소수체(TSHR)mRNA표체적영향.방법 장체외배양대서갑상선세포분위대조조、락안산Ⅰ조、락안산Ⅱ조,매조5례,분별용함0、5%、10%락안산적배양액배양96 h,수집갑상선세포,응용실시형광정량PCR검측TG、TPO、NIS、TSHR mRNA표체.결과 대조조、락안산Ⅰ조、락안산Ⅱ조TG、NIS mRNA표체분별위1.11±0.36、2.08±0.45、2.52±0.65화1.21±0.36、1.94±0.57、2.10±0.56,조간비교차이유통계학의의(F치분별위10.48、4.42,P균<0.05),기중락안산Ⅰ조、락안산Ⅱ조TG、NIS mRNA표체명현고우대조조(P균< 0.05).상술3조TPO、TSHR mRNA표체분별위1.02±0.54、1.14±0.29、1.17±0.38화0.81±0.44、0.91±0.30、0.90±0.19,조간비교차이무통계학의의(F치분별위0.18、0.26,P균>0.05).결론 락안산가능영향갑상선세포공능상관기인표체,증가전재갑상선적저존,통과작용우전빙,조절갑상선세포적전섭취공능.
Objective To investigate the effect of tyrosine on mRNA expression of thyroglobulin (TG),thyroid peroxidase (TPO),sodium/iodine symporter (NIS) and thyroid-stimulating hormone receptor (TSHR) in primary cultured rat thyroid cells.Methods Primary cultured rat thyroid cells were divided into three groups(cells from 5 rats in each group):control group,tyrosine group Ⅰ,and tyrosine group Ⅱ,and tyrosine of 0,5%,10%was added to the culture medium,and the cells were collected after cultured for 96 h,respectively.The mRNA levels of TG,TPO,NIS and TSHR of thyrocyte were determined by real-time quantitative PCR.Results The expression levels of TG,NIS mRNA in control group,tyrosine group Ⅰ and tyrosine group Ⅱ were 1.11 ± 0.36,2.08 ± 0.45,2.52 ± 0.65 and 1.21 ± 0.36,1.94 ± 0.57,2.10 ± 0.56,respectively.The differences were statistically significant (F =10.48,4.42,all P < 0.05).The mRNA levels of TG,NIS in tyrosine group Ⅰ and tyrosine group Ⅱ were significantly higher than that of control group(all P < 0.05).TPO,TSHR mRNA expression levels of control group,tyrosine group Ⅰ and tyrosine group Ⅱ were 1.02 ± 0.54,1.14 ± 0.29,1.17 ± 0.38 and 0.81 ± 0.44,0.91 ± 0.30,0.90 ± 0.19,respectively.There was no significant differences among the three groups on TPO,TSHR mRNA levels(F =0.18,0.26,all P > 0.05).Conclusions Tyrosine could effect on expression of thyroid cell function related gene and increase the storage of iodine in the thyroid by stimulating the iodine pump function,which may increase iodine intake.