CAJ | 학술논문

目的观察慢性氟中毒对大鼠肝、肾和脑皮质细胞线粒体DNA 4.8 kb大片段的影响,探讨线粒体氧化呼吸链在慢性氟中毒发病机制中的作用.方法将60只SD大鼠按体质量随机分为3组(每组20只):对照组、低氟组和高氟组,分别饮用加入0、10、50 mg/L氟化钠的自来水.6个月时,采用实时荧光定量PCR方法检测大鼠肝、肾、脑皮质细胞线粒体DNA 4.8 kb存在水平.结果低、高氟组大鼠肝脏(2.1×10-3、1.6×10-3)、肾脏(1.7×10-3、1.4×10-4)和脑皮质(1.5×10-5、1.3×10-5)细胞线粒体DNA 4.8 kb存在水平较对照组(2.9×10-3、2.0×10-3、1.1×10-4)明显下降(P均＜0.05),且高氟组肾脏DNA 4.8 kb存在水平较低氟组明显降低(P＜0.05).结论慢性氟中毒可导致大鼠肝、肾和脑皮质细胞线粒体DNA 4.8 kb大片段缺失,造成线粒体DNA结构受损,引起线粒体氧化呼吸链障碍.
목적 관찰만성불중독대대서간、신화뇌피질세포선립체DNA 4.8 kb대편단적영향,탐토선립체양화호흡련재만성불중독발병궤제중적작용.방법 장60지SD대서안체질량수궤분위3조(매조20지):대조조、저불조화고불조,분별음용가입0、10、50 mg/L불화납적자래수.6개월시,채용실시형광정량PCR방법검측대서간、신、뇌피질세포선립체DNA 4.8 kb존재수평.결과 저、고불조대서간장(2.1×10-3、1.6×10-3)、신장(1.7×10-3、1.4×10-4)화뇌피질(1.5×10-5、1.3×10-5)세포선립체DNA 4.8 kb존재수평교대조조(2.9×10-3、2.0×10-3、1.1×10-4)명현하강(P균＜0.05),차고불조신장DNA 4.8 kb존재수평교저불조명현강저(P＜0.05).결론 만성불중독가도치대서간、신화뇌피질세포선립체DNA 4.8 kb대편단결실,조성선립체DNA결구수손,인기선립체양화호흡련장애.
Objective To investigate the deletion pattern of 4.8 kb mitochondrial DNA(mito-DNA) in liver,kidney,and brain of rats with chronic fluorosis and to explore the significance of mitochondria in the pathogenesis of chronic fluorosis.Methods Sixty SD rats were randomly divided into 3 groups according to body mass (20 in each group):control,low-fluoride and high-fluoride groups,and they were fed with different concentrations of fluoride in drinking water (0,10,50 mg/L,respectively) for 6 months.Mito-DNA in liver,kidney and brain was detected by real-time PCR.Results The amounts of 4.8 kb mito-DNA in liver(2.1 × 10-3,1.6 × 10-3),kidney (1.7 × 10-3,1.4 × 10-4) and brain cortex (1.5 × 10-5,1.3 × 10-5) in low-and high-fluoride groups were significantly reduced,as compared with that of control group (2.9 × 10-3,2.0 × 10-3,1.1 × 10-4,all P ＜ 0.05).The amount of 4.8 kb mito-DNA in kidney in high-fluoride group was lower than that in low-fluoride group (P ＜ 0.05).Conclusions Excessive fluoride intake can result in missing of 4.8 kb mito-DNA in liver,kidney and brain cortex.The abnormal of mito-DNA might be related to the dysfunction of mitochondrial respiratory chain.