中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2013年
2期
133-135
,共3页
氟化物中毒%跨膜转运蛋白多糖-4%细胞核因子-κB%大鼠%肾
氟化物中毒%跨膜轉運蛋白多糖-4%細胞覈因子-κB%大鼠%腎
불화물중독%과막전운단백다당-4%세포핵인자-κB%대서%신
Fluoride poisoning%Syndecan-4%Nuclear factor kappa B%Rats%Kidney
目的 观察慢性氟中毒大鼠肾脏中跨膜转运蛋白多糖(Syndecan)-4和细胞核因子(NF)-κB的表达变化,探讨氟中毒肾损害的发病机制.方法 60只SD大鼠按体质量和性别随机分为3组:对照组,小、大剂量染氟组,自由饮用含氟化钠(NaF)＜ 0.5、10.0、50.0 mg/L的自来水,实验期为6个月.采用蛋白印迹方法和实时荧光定量PCR方法检测肾脏Syndecan-4和NF-κB的蛋白和mRNA表达.结果 与对照组大鼠肾脏中Syndecan-4蛋白表达水平和NF-κB蛋白表达水平[(100.0±8.1)％、(100.0±10.7)％]相比,小、大剂量染氟组大鼠肾脏的Syndecan-4蛋白表达水平[(198.5±5.6)％、(209.2±13.0)％]和NF-κB蛋白表达水平[(284.4±11.1)％、(343.2±2.9)％]均明显升高(P均＜0.05)；小、大剂量染氟组大鼠肾脏中Syndecan-4 mRNA表达(0.431±0.058、0.453±0.065)均高于对照组(0.128±0.026,P均＜0.05).结论 慢性氟中毒大鼠肾脏Syndecan-4的表达上调增加肾内NF-κB表达,从而引发肾脏损伤,该过程可能参与慢性氟中毒肾损伤的发生.
目的 觀察慢性氟中毒大鼠腎髒中跨膜轉運蛋白多糖(Syndecan)-4和細胞覈因子(NF)-κB的錶達變化,探討氟中毒腎損害的髮病機製.方法 60隻SD大鼠按體質量和性彆隨機分為3組:對照組,小、大劑量染氟組,自由飲用含氟化鈉(NaF)＜ 0.5、10.0、50.0 mg/L的自來水,實驗期為6箇月.採用蛋白印跡方法和實時熒光定量PCR方法檢測腎髒Syndecan-4和NF-κB的蛋白和mRNA錶達.結果 與對照組大鼠腎髒中Syndecan-4蛋白錶達水平和NF-κB蛋白錶達水平[(100.0±8.1)％、(100.0±10.7)％]相比,小、大劑量染氟組大鼠腎髒的Syndecan-4蛋白錶達水平[(198.5±5.6)％、(209.2±13.0)％]和NF-κB蛋白錶達水平[(284.4±11.1)％、(343.2±2.9)％]均明顯升高(P均＜0.05)；小、大劑量染氟組大鼠腎髒中Syndecan-4 mRNA錶達(0.431±0.058、0.453±0.065)均高于對照組(0.128±0.026,P均＜0.05).結論 慢性氟中毒大鼠腎髒Syndecan-4的錶達上調增加腎內NF-κB錶達,從而引髮腎髒損傷,該過程可能參與慢性氟中毒腎損傷的髮生.
목적 관찰만성불중독대서신장중과막전운단백다당(Syndecan)-4화세포핵인자(NF)-κB적표체변화,탐토불중독신손해적발병궤제.방법 60지SD대서안체질량화성별수궤분위3조:대조조,소、대제량염불조,자유음용함불화납(NaF)＜ 0.5、10.0、50.0 mg/L적자래수,실험기위6개월.채용단백인적방법화실시형광정량PCR방법검측신장Syndecan-4화NF-κB적단백화mRNA표체.결과 여대조조대서신장중Syndecan-4단백표체수평화NF-κB단백표체수평[(100.0±8.1)％、(100.0±10.7)％]상비,소、대제량염불조대서신장적Syndecan-4단백표체수평[(198.5±5.6)％、(209.2±13.0)％]화NF-κB단백표체수평[(284.4±11.1)％、(343.2±2.9)％]균명현승고(P균＜0.05)；소、대제량염불조대서신장중Syndecan-4 mRNA표체(0.431±0.058、0.453±0.065)균고우대조조(0.128±0.026,P균＜0.05).결론 만성불중독대서신장Syndecan-4적표체상조증가신내NF-κB표체,종이인발신장손상,해과정가능삼여만성불중독신손상적발생.
Objective Aim of the study is to investigate the expression of syndecan-4 and nuclear factor κB(NF-κB) in the kidney of rat with chronic fluorosis,and to reveal the mechanism of kidney damage resulted from the toxicity of excessive amount of fluoride.Methods According to body mass and sex,sixty SD rats were randomly divided to three groups according to body mass and fed with different contents of fluoride:control group with normal tap-water(＜ 0.5 mg/L fluoride),small dosage of fluoride exposure group (adding 10 mg/L fluoride in tap-water) and large dosage of fluoride exposure group (50 mg/L fluoride) for six months.The protein level of syndecan-4 and NF-κB in the kidney was detected by Western blotting and syndecan-4 mRNA level by quantitative real time PCR.Results As compared to the control group[(100.0 + 8.1)％],the expression of syndecan-4 at protein level in the kidney of rat was significantly increased in the small dosage of fluoride exposure group [(198.5 + 5.6)％,P ＜ 0.05] and large dosage of fluoride exposure group [(209.2 + 13.0)％,P ＜ 0.05]; the protein levels of NF-κB in the small dosage of fluoride exposure group[(284.4 + 11.1)％,P ＜ 0.05] and in the large dosage of fluoride exposure group[(343.2 + 2.9)％,P ＜ 0.05] were significantly increased than that of the control group[(100.0 ± 10.7)％].The mRNA levels of syndecan-4 in the kidney in the small dosage of fluoride exposure group and large dosage of fluoride exposure group(0.431 + 0.058 and 0.453 ± 0.065,both P ＜ 0.05,respectively) were significantly increased than that of the control(0.128 + 0.026).Conclusions The increased expression of NF-κB in the kidney is induced by increased expression of syndecan-4,which may be involved in kidney damage of chronic fluorosis.
