中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2013年
2期
146-148
,共3页
氟化钠%小鼠%雌激素受体
氟化鈉%小鼠%雌激素受體
불화납%소서%자격소수체
Sodium fluoride%Mice%Estrogen receptor
目的 观察亚慢性染氟(氟化钠)对雌性小鼠雌激素受体(ER)mRNA表达的影响.方法 雌性小鼠40只,按体质量随机分成4组:对照组、低氟组、中氟组、高氟组,每组10只.分别饮用0、1、5、25 mg/L氟化钠溶液,喂养12周后,RT-PCR法检测小鼠卵巢中ERα、ERβ mRNA表达.结果 对照组、低氟组、中氟组、高氟组小鼠卵巢组织ERα、ERβ mRNA表达分别为0.7028±0.0474、0.7195±0.0552、0.6479±0.0590、0.5684±0.0513,0.8418±0.0131、0.7729±0.0974、0.7610±0.0984、0.8026±0.0234.其中高氟组小鼠卵巢组织ERαmRNA表达与对照组比较,差异有统计学意义(P＜0.05).结论 亚慢性染氟可导致卵巢组织中雌激素受体表达下降,后者对雌性小鼠的生殖发育可能有一定的影响作用.
目的 觀察亞慢性染氟(氟化鈉)對雌性小鼠雌激素受體(ER)mRNA錶達的影響.方法 雌性小鼠40隻,按體質量隨機分成4組:對照組、低氟組、中氟組、高氟組,每組10隻.分彆飲用0、1、5、25 mg/L氟化鈉溶液,餵養12週後,RT-PCR法檢測小鼠卵巢中ERα、ERβ mRNA錶達.結果 對照組、低氟組、中氟組、高氟組小鼠卵巢組織ERα、ERβ mRNA錶達分彆為0.7028±0.0474、0.7195±0.0552、0.6479±0.0590、0.5684±0.0513,0.8418±0.0131、0.7729±0.0974、0.7610±0.0984、0.8026±0.0234.其中高氟組小鼠卵巢組織ERαmRNA錶達與對照組比較,差異有統計學意義(P＜0.05).結論 亞慢性染氟可導緻卵巢組織中雌激素受體錶達下降,後者對雌性小鼠的生殖髮育可能有一定的影響作用.
목적 관찰아만성염불(불화납)대자성소서자격소수체(ER)mRNA표체적영향.방법 자성소서40지,안체질량수궤분성4조:대조조、저불조、중불조、고불조,매조10지.분별음용0、1、5、25 mg/L불화납용액,위양12주후,RT-PCR법검측소서란소중ERα、ERβ mRNA표체.결과 대조조、저불조、중불조、고불조소서란소조직ERα、ERβ mRNA표체분별위0.7028±0.0474、0.7195±0.0552、0.6479±0.0590、0.5684±0.0513,0.8418±0.0131、0.7729±0.0974、0.7610±0.0984、0.8026±0.0234.기중고불조소서란소조직ERαmRNA표체여대조조비교,차이유통계학의의(P＜0.05).결론 아만성염불가도치란소조직중자격소수체표체하강,후자대자성소서적생식발육가능유일정적영향작용.
Objective To study the subchronic toxicity of sodium fluoride on mRNA expression of estrogen receptor(ER) in female mice.Methods Forty female mice were randomly divided into 4 groups according to body mass,10 in each group,and exposed to sodium fluoride solution(0,1,5,25 mg/L) through drinking water in control,low-dose,medium-dose and high-dose groups for 12 weeks.The expression levels of ERα,ER3 mRNA in ovarian tissues were determined by RT-PCR method.Results The relative expression levels of ERα,ERβ mRNA in control,low-,medium-and high-dose groups were 0.7028 + 0.0474,0.7195 ± 0.0552,0.6479 ± 0.0590,0.5684 ± 0.0513 and 0.8418 ± 0.0131,0.7729 ± 0.0974,0.7610 ± 0.0984,0.8026 ± 0.0234,respectively.The difference between high-dose and control groups of the expression level of ERα was statistically significant (P ＜ 0.05).Conclusions The subchronic toxicity of fluoride can decrease the expression of estrogen receptor in ovarian tissue,which may have a certain effect on reproductive development of female mice.
