中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2013年
2期
155-158
,共4页
李金华%李远慧%孔祥照%李娜%金婷婷
李金華%李遠慧%孔祥照%李娜%金婷婷
리금화%리원혜%공상조%리나%금정정
砷中毒%大鼠%空肠%P物质%生长抑素
砷中毒%大鼠%空腸%P物質%生長抑素
신중독%대서%공장%P물질%생장억소
Arsenic poisoning%Rats%Jejunum%Substance P%Somatostatin
目的 探讨慢性砷中毒对大鼠空肠P物质(sP)、生长抑素(SS)表达的影响及其与空肠形态结构变化的关系.方法 将45只清洁级SD大鼠按性别和体质量随机分为对照(0.0 mg.kg-1.d-1)、低砷(0.4 mg.kg-1·d-1)、高砷(10.0 mg·kg-1·d-1)组,每组15只.分别自由饮用砷(三氧化二砷,As2O3)含量为0、2、50 mg/L的蒸馏水溶液,连续染毒4个月.采用HE染色、免疫组化链霉亲和素-生物素复合物(SABC)法光镜下观察空肠形态结构变化及SP、SS阳性细胞数,Biomias图像分析软件测定SP、SS阳性细胞平均灰度值.结果 光镜下,慢性砷中毒大鼠部分空肠绒毛不规则；部分纹状缘脱落,不整齐；杯状细胞增多；固有层内炎性细胞浸润；小肠腺部分细胞出现空泡.3组大鼠空肠SP、SS阳性细胞计数比较,差异有统计学意义(F值分别为608.54、227.59,P均＜0.05),其中低、高砷组[(1.85±0.25)、(1.83±0.24)个/视野和(4.24±0.33)、(3.31±0.41)个/视野]明显高于对照组[(0.94±0.21)、(1.14±0.14)个/视野],且高砷组明显高于低砷组(P均＜0.05).3组大鼠空肠SP、SS阳性细胞平均灰度值比较,差异有统计学意义(F值分别为68.43、26.57,P均＜0.05),其中低、高砷组(125.13±2.35、131.28±5.66和118.30±4.58、124.03±3.94)明显低于对照组(133.76±3.61、137.57±5.49),且高砷组明显低于低砷组(P均＜ 0.05).结论 慢性砷中毒大鼠空肠黏膜损伤及形态结构改变与SP、SS表达上调有关.
目的 探討慢性砷中毒對大鼠空腸P物質(sP)、生長抑素(SS)錶達的影響及其與空腸形態結構變化的關繫.方法 將45隻清潔級SD大鼠按性彆和體質量隨機分為對照(0.0 mg.kg-1.d-1)、低砷(0.4 mg.kg-1·d-1)、高砷(10.0 mg·kg-1·d-1)組,每組15隻.分彆自由飲用砷(三氧化二砷,As2O3)含量為0、2、50 mg/L的蒸餾水溶液,連續染毒4箇月.採用HE染色、免疫組化鏈黴親和素-生物素複閤物(SABC)法光鏡下觀察空腸形態結構變化及SP、SS暘性細胞數,Biomias圖像分析軟件測定SP、SS暘性細胞平均灰度值.結果 光鏡下,慢性砷中毒大鼠部分空腸絨毛不規則；部分紋狀緣脫落,不整齊；杯狀細胞增多；固有層內炎性細胞浸潤；小腸腺部分細胞齣現空泡.3組大鼠空腸SP、SS暘性細胞計數比較,差異有統計學意義(F值分彆為608.54、227.59,P均＜0.05),其中低、高砷組[(1.85±0.25)、(1.83±0.24)箇/視野和(4.24±0.33)、(3.31±0.41)箇/視野]明顯高于對照組[(0.94±0.21)、(1.14±0.14)箇/視野],且高砷組明顯高于低砷組(P均＜0.05).3組大鼠空腸SP、SS暘性細胞平均灰度值比較,差異有統計學意義(F值分彆為68.43、26.57,P均＜0.05),其中低、高砷組(125.13±2.35、131.28±5.66和118.30±4.58、124.03±3.94)明顯低于對照組(133.76±3.61、137.57±5.49),且高砷組明顯低于低砷組(P均＜ 0.05).結論 慢性砷中毒大鼠空腸黏膜損傷及形態結構改變與SP、SS錶達上調有關.
목적 탐토만성신중독대대서공장P물질(sP)、생장억소(SS)표체적영향급기여공장형태결구변화적관계.방법 장45지청길급SD대서안성별화체질량수궤분위대조(0.0 mg.kg-1.d-1)、저신(0.4 mg.kg-1·d-1)、고신(10.0 mg·kg-1·d-1)조,매조15지.분별자유음용신(삼양화이신,As2O3)함량위0、2、50 mg/L적증류수용액,련속염독4개월.채용HE염색、면역조화련매친화소-생물소복합물(SABC)법광경하관찰공장형태결구변화급SP、SS양성세포수,Biomias도상분석연건측정SP、SS양성세포평균회도치.결과 광경하,만성신중독대서부분공장융모불규칙；부분문상연탈락,불정제；배상세포증다；고유층내염성세포침윤；소장선부분세포출현공포.3조대서공장SP、SS양성세포계수비교,차이유통계학의의(F치분별위608.54、227.59,P균＜0.05),기중저、고신조[(1.85±0.25)、(1.83±0.24)개/시야화(4.24±0.33)、(3.31±0.41)개/시야]명현고우대조조[(0.94±0.21)、(1.14±0.14)개/시야],차고신조명현고우저신조(P균＜0.05).3조대서공장SP、SS양성세포평균회도치비교,차이유통계학의의(F치분별위68.43、26.57,P균＜0.05),기중저、고신조(125.13±2.35、131.28±5.66화118.30±4.58、124.03±3.94)명현저우대조조(133.76±3.61、137.57±5.49),차고신조명현저우저신조(P균＜ 0.05).결론 만성신중독대서공장점막손상급형태결구개변여SP、SS표체상조유관.
Objective To explore the relationship between substance P(SP),somatostatin(SS) expression and change of morphology structure in jejunum of arsenism rats.Methods Acoording to sex and body mass,forty five clean grade SD rats were divided into control(0.0 mg.kg-1.d-1),low-dose arsenic(0.4 mg.kg-1.d-1) and high-dose arsenic(10.0 mg.kg-1.d-1) groups,n =15.The rats in low-and high-dose groups were treated with As2O3(2,50 mg/L) through drinking water for 4 months,respectively.Morphology changes of jejunum were observed by histological technique-HE staining and SABC immunohistochemistry.SP and SS positive cells in the jejunum were observed and counted,and its average gray value was analyzed with image analysis software (Biomias).Results Some jejunal villi were irregular in arsenism rats; with some brush border loss and irregular; goblet cells increased; infiltration of inflammatory cells in the lamina propria; and vacuoles in some intestinal gland cells.The differences of SP and SS positive cells between groups were statistically significant (F =608.54,227.59,all P ＜0.05).Compared with the control group (0.94 + 0.21,1.14 + 0.14),SP and SS positive cells in low-and highdose arsenic groups(1.85 + 0.25,1.83 + 0.24 and 4.24 + 0.33,3.31 ± 0.41) were significantly higher(all P ＜0.05),and high-dose arsenic group was significantly higher than the low-dose arsenic group(all P ＜ 0.05).The differences of average gray values of SP and SS positive cells between groups were statistically significant(F =68.43,26.57,all P ＜ 0.05).Compared with the control group(133.76 ± 3.61,137.57 ± 5.49),SP and SS positive cells in low-and high-dose arsenic groups(125.13 + 2.35,131.28 ± 5.66 and 118.30 ± 4.58,124.03 ± 3.94) were significantly lower(all P ＜ 0.05),and high-dose arsenic group was significantly lower than the low-dose arsenic group (all P ＜ 0.05).Conclusions Up-regulation of SP,SS may be related to jejunal mucosal injury and morphology structure in arsenic poisoning rats.
