CAJ | 학술논문

目的鉴定与分析青海湟源县一起人间鼠疫死亡病例的菌株感染来源.方法采集2011年青海湟源县一起人间鼠疫死亡病例的脏器、淋巴液及血液标本进行鼠疫菌分离培养、生化鉴定、毒力鉴定及基因差异区段(DFR)分析等.结果从脏器标本中分离到鼠疫菌,生化鉴定为青藏高原旱獭型;菌株具备F1、P1、Pgm、Pst毒力因子;小鼠绝对致死量(LD100)为1000个菌,半数致死量(LD50)为10个菌.DFR基因分型为5型.结论死亡病例分离菌株为青藏高原型鼠疫菌.在传统流行病学调查基础上,结合使用分子流行病学技术进行分析,对及时发现疫情、追溯感染来源具有重要的现实意义.
목적 감정여분석청해황원현일기인간서역사망병례적균주감염래원.방법 채집2011년청해황원현일기인간서역사망병례적장기、림파액급혈액표본진행서역균분리배양、생화감정、독력감정급기인차이구단(DFR)분석등.결과 종장기표본중분리도서역균,생화감정위청장고원한달형;균주구비F1、P1、Pgm、Pst독력인자;소서절대치사량(LD100)위1000개균,반수치사량(LD50)위10개균.DFR기인분형위5형.결론 사망병례분리균주위청장고원형서역균.재전통류행병학조사기출상,결합사용분자류행병학기술진행분석,대급시발현역정、추소감염래원구유중요적현실의의.
Objective To identificate and analyze the source of Yersinia pestis (Y.pestis) from a human plague death case in Huangyuan County,Qinghai Province.Methods The organs,lymph and blood samples of this human plague death case in Huangyuan County,Qinghai Province in 2011 were collected for laboratory tests including isolating and culturing Y.pestis,biochemical identification,toxicity identification and genes' different region(DFR) analysis.Results Y.pestis was separated from organ samples,and it was identified as Qinghai-Tibet Plateau Marmot strain through biochemical identification test; it had F1,P1,Pgm and Pst four virulence factors; the mouse virulence test showed that the absolute lethal dose(LD100) was 1000 Y.pestis,and the median lethal dose(LD50) was 10 Y.pestis; DFR genotyping analysis showed that the genotype was type 5.Conclusions The strain separated from the death case is Qinghai-Tibet Plateau Marmot strain.Traditional epidemiological investigation combined with molecular epidemiological techniques has an important practical significance for timely detection of outbreaks,and trace the source of infection.