中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2013年
6期
605-607
,共3页
王鹏%叶蕊%石丽媛%崔志刚%杨光璨%董珊珊%郭英%李伟%宋志忠
王鵬%葉蕊%石麗媛%崔誌剛%楊光璨%董珊珊%郭英%李偉%宋誌忠
왕붕%협예%석려원%최지강%양광찬%동산산%곽영%리위%송지충
耶尔森菌,鼠疫%电泳,凝胶,脉冲场%云南
耶爾森菌,鼠疫%電泳,凝膠,脈遲場%雲南
야이삼균,서역%전영,응효,맥충장%운남
Yersinia pestis%Electrophoresis,gel,pulsed-field%Yunnan
目的 构建云南省鼠疫菌株脉冲场凝胶电泳(PFGE)图谱库,为云南鼠疫的溯源及分型防控提供依据.方法 选取云南省家、野鼠型以及玉龙鼠疫菌株共29株,分别采用Asc Ⅰ和Fse Ⅰ两种内切酶对菌株进行酶切分析,并使用BioNumerics 6.6软件对图谱聚类.结果 PFGE分析可以将29株鼠疫菌分为20个基因型,这些基因型又归为3大簇,即家鼠基因簇、野鼠基因簇以及玉龙基因簇,家鼠基因簇又可分为德宏亚簇、文山亚簇及临沧亚簇.结论 云南鼠疫菌PFGE分子分型具有区域聚集性;PFGE基因型与菌株生态型能够很好地对应;玉龙鼠疫菌是云南一个独立的PFGE基因型.
目的 構建雲南省鼠疫菌株脈遲場凝膠電泳(PFGE)圖譜庫,為雲南鼠疫的溯源及分型防控提供依據.方法 選取雲南省傢、野鼠型以及玉龍鼠疫菌株共29株,分彆採用Asc Ⅰ和Fse Ⅰ兩種內切酶對菌株進行酶切分析,併使用BioNumerics 6.6軟件對圖譜聚類.結果 PFGE分析可以將29株鼠疫菌分為20箇基因型,這些基因型又歸為3大簇,即傢鼠基因簇、野鼠基因簇以及玉龍基因簇,傢鼠基因簇又可分為德宏亞簇、文山亞簇及臨滄亞簇.結論 雲南鼠疫菌PFGE分子分型具有區域聚集性;PFGE基因型與菌株生態型能夠很好地對應;玉龍鼠疫菌是雲南一箇獨立的PFGE基因型.
목적 구건운남성서역균주맥충장응효전영(PFGE)도보고,위운남서역적소원급분형방공제공의거.방법 선취운남성가、야서형이급옥룡서역균주공29주,분별채용Asc Ⅰ화Fse Ⅰ량충내절매대균주진행매절분석,병사용BioNumerics 6.6연건대도보취류.결과 PFGE분석가이장29주서역균분위20개기인형,저사기인형우귀위3대족,즉가서기인족、야서기인족이급옥룡기인족,가서기인족우가분위덕굉아족、문산아족급림창아족.결론 운남서역균PFGE분자분형구유구역취집성;PFGE기인형여균주생태형능구흔호지대응;옥룡서역균시운남일개독립적PFGE기인형.
Objective To construct pulsed-field gel electrophoresis (PFGE) library typing of Yersinia pestis in Yunnan Province,and to provide a basis for control of the disease through originating and typing the disease.Methods Twenty-nine strains isolated from Rattus flavipectus plague focus,Apodemus chevrieriEothenomys miletus plague focus and Yulong plague focus in Yunnan Province were involved in this study.Two kinds of endonucleases of Asc Ⅰ and Fse Ⅰ were used in PFGE analysis,and clustering patterns were draw using BioNumerics 6.6 software.Results The 29 strains of Yersinia pestis can be divided into 20 genotypes by PFGE.These genotypes were grouped into three major clusters,namely Rattus flavipectus gene cluster,Apodemus chevrieri-Eothenomys miletus gene cluster and Yulong gene cluster.Rattus flavipectus gene cluster could be further divided into 3 sub-clusters of Dehong,Wenshan and Lincang.Conclusions There are regional aggregations of molecular typing of Yersinia pestis in Yunnan Province by PFGE; and the result of PFGE genotyping is well related to that of ecological typing; Yulong plague is an independent PFGE pattern.
