中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2013年
6期
612-615
,共4页
冯林森%吴泽生%李靖娟%张爱芬%吴皎%张东葵%段艳萍%王金德
馮林森%吳澤生%李靖娟%張愛芬%吳皎%張東葵%段豔萍%王金德
풍림삼%오택생%리정연%장애분%오교%장동규%단염평%왕금덕
慢性血吸虫病%结肠腺%咬合蛋白%紧密连接蛋白-1
慢性血吸蟲病%結腸腺%咬閤蛋白%緊密連接蛋白-1
만성혈흡충병%결장선%교합단백%긴밀련접단백-1
Chronic schistosomiasis%Colonic gland%Occludin%Zonula occludens-1
目的 探讨慢性血吸虫病患者结肠腺咬合蛋白(occludin)与紧密连接蛋白-1 (ZO-1)的表达及其临床意义.方法 选取50例慢性血吸虫病患者及9例正常结肠黏膜标本,采用免疫组织化学(SABC)染色法,检测occludin与ZO-1在结肠腺上皮细胞的表达情况.结果 与对照组比较,慢性血吸虫病组结肠腺结构不完整、腺体萎缩;慢性血吸虫病组结肠腺上皮细胞occludin与ZO-1阳性颗粒平均灰度值(159.26±6.57、166.07±5.44)明显高于对照组(155.53±5.90、162.16±5.14,P＜0.05),阳性单位值(37.27±2.63、34.69±2.19)明显低于对照组(38.85±2.42、36.37±2.11,P＜0.05);慢性血吸虫病组结肠腺occludin与ZO-1表达量的减少与性别及年龄无关(P＞0.05).结论 血吸虫感染可以损伤结肠腺上皮细胞间的紧密连接蛋白,从而破坏患者结肠黏膜屏障的完整性.
目的 探討慢性血吸蟲病患者結腸腺咬閤蛋白(occludin)與緊密連接蛋白-1 (ZO-1)的錶達及其臨床意義.方法 選取50例慢性血吸蟲病患者及9例正常結腸黏膜標本,採用免疫組織化學(SABC)染色法,檢測occludin與ZO-1在結腸腺上皮細胞的錶達情況.結果 與對照組比較,慢性血吸蟲病組結腸腺結構不完整、腺體萎縮;慢性血吸蟲病組結腸腺上皮細胞occludin與ZO-1暘性顆粒平均灰度值(159.26±6.57、166.07±5.44)明顯高于對照組(155.53±5.90、162.16±5.14,P＜0.05),暘性單位值(37.27±2.63、34.69±2.19)明顯低于對照組(38.85±2.42、36.37±2.11,P＜0.05);慢性血吸蟲病組結腸腺occludin與ZO-1錶達量的減少與性彆及年齡無關(P＞0.05).結論 血吸蟲感染可以損傷結腸腺上皮細胞間的緊密連接蛋白,從而破壞患者結腸黏膜屏障的完整性.
목적 탐토만성혈흡충병환자결장선교합단백(occludin)여긴밀련접단백-1 (ZO-1)적표체급기림상의의.방법 선취50례만성혈흡충병환자급9례정상결장점막표본,채용면역조직화학(SABC)염색법,검측occludin여ZO-1재결장선상피세포적표체정황.결과 여대조조비교,만성혈흡충병조결장선결구불완정、선체위축;만성혈흡충병조결장선상피세포occludin여ZO-1양성과립평균회도치(159.26±6.57、166.07±5.44)명현고우대조조(155.53±5.90、162.16±5.14,P＜0.05),양성단위치(37.27±2.63、34.69±2.19)명현저우대조조(38.85±2.42、36.37±2.11,P＜0.05);만성혈흡충병조결장선occludin여ZO-1표체량적감소여성별급년령무관(P＞0.05).결론 혈흡충감염가이손상결장선상피세포간적긴밀련접단백,종이파배환자결장점막병장적완정성.
Objective To study the expression of occludin and zonula occludens-1 (ZO-1) in patients with chronic schistosomiasis and its clinical significance.Methods Colonic mucosa specimens of 50 patients with chronic schistosomiasis and 9 cases of normal people(as a control group) were selected and the expressions of occludin and ZO-1 in colonic epithelial cells were determined using immunohistochemical staining(SABC).Results Compared with the normal control group,the structure of colonic gland was incomplete with glandular atrophy,mean gray values(159.26 ± 6.57,166.07 ± 5.44) of the positive particles of occludin and ZO-1 of colonic epithelial cells in the chronic schistosomiasis group were significantly higher than those of the normal control group(155.53 ± 5.90,162.16 ± 5.14,P ＜ 0.05),but positive unit values(37.27 ± 2.63,34.69 ± 2.19) were significantly lower than those of the control group(38.85 ± 2.42,36.37 ± 2.11,P ＜ 0.05); reduced expression of occludin and ZO-1 in colonic gland of the patients with chronic schistosomiasis had nothing to do with sex or age (P ＞ 0.05).Conclusions Infection of schistosome can reduce the expression of occludin and ZO-1 which can damage the tight junction between colonic epithelium cells,thereby undermining the integrity of the colonic mucosa barrier.
