中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2013年
6期
636-638
,共3页
孙美娜%赵汉东%张一彤%李晖
孫美娜%趙漢東%張一彤%李暉
손미나%조한동%장일동%리휘
硒%硒结合蛋白1%硒蛋白P
硒%硒結閤蛋白1%硒蛋白P
서%서결합단백1%서단백P
Selenium%Selenium-binding protein 1%Selenoprotein P
目的 探讨饮食低硒对小鼠肝、肾、脑组织硒结合蛋白1(SBP1)和硒蛋白P(Sel P)表达的影响.方法 将C57BL/6小鼠按体质量随机分为4组:对照组和低硒4、12、24周组,每组10只,雌雄各半.对照组饲以普通饲料(含硒量为0.300 mg/kg),饮用蒸馏水,第12周断颈处死;低硒各组饲以低硒饲料(含硒量为0.015mg/kg),分别于第4、12、24周时断颈处死.采用蛋白印迹(Western blot)法检测小鼠肝、肾、脑组织SBP1与SelP蛋白表达.结果 低硒4、12、24周组小鼠肝组织中SBP1和Sel P蛋白表达分别为0.11±0.01、0.36±0.01、0.59±0.02和0.41±0.01、0.39±0.02、0.25±0.02;肾脏组织中分别为0.60±0.03、0.20±0.02、0.03±0.01和0.88±0.01、0.73±0.03、0.85±0.02;脑组织中分别为0.54±0.03、0.11±0.01、0.01±0.01和0.50±0.02、0.49±0.03、0.38±0.02.低硒各组小鼠肝脏、肾脏、脑组织SBP1、Sel P蛋白表达与对照组(1.00±0.00、1.00±0.00)比较明显降低(P均＜0.05),其中各脏器中SBP1的表达量随低硒喂养时间的延长各有不同,肝脏先降低后回升,肾脏和脑组织则持续降低.结论 低硒饮食对小鼠肝、肾、脑组织内Sel P和SBP1的表达量有一定的影响.
目的 探討飲食低硒對小鼠肝、腎、腦組織硒結閤蛋白1(SBP1)和硒蛋白P(Sel P)錶達的影響.方法 將C57BL/6小鼠按體質量隨機分為4組:對照組和低硒4、12、24週組,每組10隻,雌雄各半.對照組飼以普通飼料(含硒量為0.300 mg/kg),飲用蒸餾水,第12週斷頸處死;低硒各組飼以低硒飼料(含硒量為0.015mg/kg),分彆于第4、12、24週時斷頸處死.採用蛋白印跡(Western blot)法檢測小鼠肝、腎、腦組織SBP1與SelP蛋白錶達.結果 低硒4、12、24週組小鼠肝組織中SBP1和Sel P蛋白錶達分彆為0.11±0.01、0.36±0.01、0.59±0.02和0.41±0.01、0.39±0.02、0.25±0.02;腎髒組織中分彆為0.60±0.03、0.20±0.02、0.03±0.01和0.88±0.01、0.73±0.03、0.85±0.02;腦組織中分彆為0.54±0.03、0.11±0.01、0.01±0.01和0.50±0.02、0.49±0.03、0.38±0.02.低硒各組小鼠肝髒、腎髒、腦組織SBP1、Sel P蛋白錶達與對照組(1.00±0.00、1.00±0.00)比較明顯降低(P均＜0.05),其中各髒器中SBP1的錶達量隨低硒餵養時間的延長各有不同,肝髒先降低後迴升,腎髒和腦組織則持續降低.結論 低硒飲食對小鼠肝、腎、腦組織內Sel P和SBP1的錶達量有一定的影響.
목적 탐토음식저서대소서간、신、뇌조직서결합단백1(SBP1)화서단백P(Sel P)표체적영향.방법 장C57BL/6소서안체질량수궤분위4조:대조조화저서4、12、24주조,매조10지,자웅각반.대조조사이보통사료(함서량위0.300 mg/kg),음용증류수,제12주단경처사;저서각조사이저서사료(함서량위0.015mg/kg),분별우제4、12、24주시단경처사.채용단백인적(Western blot)법검측소서간、신、뇌조직SBP1여SelP단백표체.결과 저서4、12、24주조소서간조직중SBP1화Sel P단백표체분별위0.11±0.01、0.36±0.01、0.59±0.02화0.41±0.01、0.39±0.02、0.25±0.02;신장조직중분별위0.60±0.03、0.20±0.02、0.03±0.01화0.88±0.01、0.73±0.03、0.85±0.02;뇌조직중분별위0.54±0.03、0.11±0.01、0.01±0.01화0.50±0.02、0.49±0.03、0.38±0.02.저서각조소서간장、신장、뇌조직SBP1、Sel P단백표체여대조조(1.00±0.00、1.00±0.00)비교명현강저(P균＜0.05),기중각장기중SBP1적표체량수저서위양시간적연장각유불동,간장선강저후회승,신장화뇌조직칙지속강저.결론 저서음식대소서간、신、뇌조직내Sel P화SBP1적표체량유일정적영향.
Objective To explore the influence of low-selenium diet on expression of selenium-binding protein 1 (SBP1) and selenoprotein P (Sel P) in liver,kidney and brain tissues.Methods C57BL/6 mice were randomly divided into four groups according to body weight:control group,low-selenium treatment for 4-,12-and 24-week groups,10 mice in each group,half male and half female.The control group was fed with normal diet (selenium content was 0.300 mg/kg),distilled water,and sacrificed at the 12th week; low-selenium treatment groups were fed with low-selenium diet(selenium content was 0.015 mg/kg),then sacrificed at the 4th,12th and 24th weeks,respectively.Expressions of SBP1 and Sel P in mouse liver,kidney and brain tissues were determined by Western blotting.Results Expressions of SBP1 and Sel P in low-selenium feed mouse liver tissue at the 4th,12th and 24th weeks were,respectively,as follows 0.11 ± 0.01,0.36 ± 0.01,0.59 ± 0.02 and 0.41 ± 0.01,0.39 ± 0.02,0.25 ± 0.02;in kidney,respectively,as follows 0.60 ± 0.03,0.20 ± 0.02,0.03 ± 0.01 and 0.88 ± 0.01,0.73 ± 0.03,0.85 ± 0.02; in brain,respectively,as follows 0.54 ± 0.03,0.11 ± 0.01,0.01 ± 0.01 and 0.50 ± 0.02,0.49 ± 0.03,0.38 ± 0.02.Expression of Sel P in low-selenium feed mouse liver,kidney and brain tissues was significantly decreased as compared to that of control group(1.00 ± 0.00,1.00 ± 0.00,all P ＜ 0.05),but SBP1 content was reduced at first and then rebounded in kidney,and was in decreasing trend in liver and brain tissues.Conclusion Low-selenium diet has a certain effect on expression of SBP1 and Sel P in mouse liver,kidney and brain tissues.
