中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2013年
6期
694-696
,共3页
马巍%李秀维%刘诗川%李淑华%刘列钧
馬巍%李秀維%劉詩川%李淑華%劉列鈞
마외%리수유%류시천%리숙화%류렬균
尿%碘%方法%消化条件%过硫酸铵
尿%碘%方法%消化條件%過硫痠銨
뇨%전%방법%소화조건%과류산안
Urine%Iodine%Methods%Digestion conditions%Ammonium persulfate
目的 研究样品消化条件对尿碘测定结果的影响,为2012年新修订尿碘测定标准方法的应用提供支持.方法 根据新修订的尿碘测定标准方法,使用3份标准物质[GWB09108k、GWB09109f、GWB09110m,碘含量分别为(68.2±9.0)、(138.0±10.0)、(221.0±10.0)μg/L]、5份尿样(碘含量为100～300 μg/L),对消解液的浓度(0.8 ～ 1.3 mol/L过硫酸铵溶液,每组间隔0.1 mol/L)、消解设备(恒温消解仪和电热干燥箱)以及消化后放置时间(0.5、1.0、2.0、4.0、22.0 h)等条件进行研究.结果 0.9、1.0、1.1 mol/L过硫酸铵消解液3组间测定结果比较差异无统计学意义(P＞ 0.05);恒温消解仪与电热干燥箱消化效果比较,除1份低浓度标准物质(GBW09108k)外,其余标准物质及样品测定结果的差异无统计学意义(P＞0.05);消化后样品放置0.5～ 22.0 h各组间测定结果的差异无统计学意义(P＞0.05).结论 过硫酸铵消解液适宜浓度范围为0.9～1.1 mol/L;消化时推荐使用恒温消解仪,在缺乏时,可使用电热干燥箱替代;消化后的样品放置0.5～ 22 h不影响测定结果.
目的 研究樣品消化條件對尿碘測定結果的影響,為2012年新脩訂尿碘測定標準方法的應用提供支持.方法 根據新脩訂的尿碘測定標準方法,使用3份標準物質[GWB09108k、GWB09109f、GWB09110m,碘含量分彆為(68.2±9.0)、(138.0±10.0)、(221.0±10.0)μg/L]、5份尿樣(碘含量為100～300 μg/L),對消解液的濃度(0.8 ～ 1.3 mol/L過硫痠銨溶液,每組間隔0.1 mol/L)、消解設備(恆溫消解儀和電熱榦燥箱)以及消化後放置時間(0.5、1.0、2.0、4.0、22.0 h)等條件進行研究.結果 0.9、1.0、1.1 mol/L過硫痠銨消解液3組間測定結果比較差異無統計學意義(P＞ 0.05);恆溫消解儀與電熱榦燥箱消化效果比較,除1份低濃度標準物質(GBW09108k)外,其餘標準物質及樣品測定結果的差異無統計學意義(P＞0.05);消化後樣品放置0.5～ 22.0 h各組間測定結果的差異無統計學意義(P＞0.05).結論 過硫痠銨消解液適宜濃度範圍為0.9～1.1 mol/L;消化時推薦使用恆溫消解儀,在缺乏時,可使用電熱榦燥箱替代;消化後的樣品放置0.5～ 22 h不影響測定結果.
목적 연구양품소화조건대뇨전측정결과적영향,위2012년신수정뇨전측정표준방법적응용제공지지.방법 근거신수정적뇨전측정표준방법,사용3빈표준물질[GWB09108k、GWB09109f、GWB09110m,전함량분별위(68.2±9.0)、(138.0±10.0)、(221.0±10.0)μg/L]、5빈뇨양(전함량위100～300 μg/L),대소해액적농도(0.8 ～ 1.3 mol/L과류산안용액,매조간격0.1 mol/L)、소해설비(항온소해의화전열간조상)이급소화후방치시간(0.5、1.0、2.0、4.0、22.0 h)등조건진행연구.결과 0.9、1.0、1.1 mol/L과류산안소해액3조간측정결과비교차이무통계학의의(P＞ 0.05);항온소해의여전열간조상소화효과비교,제1빈저농도표준물질(GBW09108k)외,기여표준물질급양품측정결과적차이무통계학의의(P＞0.05);소화후양품방치0.5～ 22.0 h각조간측정결과적차이무통계학의의(P＞0.05).결론 과류산안소해액괄의농도범위위0.9～1.1 mol/L;소화시추천사용항온소해의,재결핍시,가사용전열간조상체대;소화후적양품방치0.5～ 22 h불영향측정결과.
Objective To study the effects of sample digestion conditions on measurement results of urinary iodine determined by As(Ⅲ)-Ce4+ catalytic spectrophotometry with ammonium persulfate digestion,and to promote the application of newly revised (the 2012 edition) national standard method for determination of urinary iodine.Methods According to the newly revised national standard method,various digestion conditions,such as ammonium persulfate concentration (0.8-1.3 mol/L,group interval 0.1),digestion instruments (heating block and drying oven) and standing time after digestion(0.5,1.0,2.0,4.0 and 22.0 h),were studied.The samples included 3 standard materials,which were GWB09108k,GWB09109f and GWB09110m containing iodine of (68.2 ± 9.0),(138.0 ± 10.0) and (221.0 ± 10.0) μg/L,and 5 urine samples with iodine concentration of 100-300 μg/L.Results Measurement results among the three groups of 0.9,1.0 and 1.1 mol/L ammonium persulfate digestion fluid showed no significant difference(P ＞ 0.05).The digestive effect showed no significant difference between heating block and drying oven (P ＞ 0.05) except one standard material in low concentration (GBW09108k).After digestion,samples were placed 0.5-22.0 h,the measurement results between groups showed no significant difference (P ＞ 0.05).Conclusions Appropriate concentrations of ammonium persulfate are from 0.9 mol/L to 1.1 mol/L.Heating block is recommended for the digestion,however,when absent,drying oven can be used alternatively.The standing times from 0.5 h to 22 h after digestion have not affected the measurement results.
