中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2014年
3期
268-271
,共4页
潘际刚%楼迪栋%刘艳洁%魏娜%刘咏妍%官志忠
潘際剛%樓迪棟%劉豔潔%魏娜%劉詠妍%官誌忠
반제강%루적동%류염길%위나%류영연%관지충
氟中毒,牙%胱硫醚-β-合成酶%硫化氢%大鼠
氟中毒,牙%胱硫醚-β-閤成酶%硫化氫%大鼠
불중독,아%광류미-β-합성매%류화경%대서
Fluorosis,dental%Cystathionine-beta-synthase%Hydrogen sulfide%Rats
目的 观察慢性氟中毒对大鼠内源性胱硫醚-β-合成酶(CBS)及硫化氢(H2S)水平的影响.方法 48只SD大鼠(体质量105～180 g)按体质量采用随机数字表法分为3组,每组16只大鼠(雌雄各半),其中对照组、低氟组、高氟组大鼠饲料氟含量分别为9.80、15.40、23.80 mg/kg.染氟6个月后,氟离子选择电极法检测大鼠尿氟及骨氟;亚甲基蓝法检测大鼠血清及脑组织H2S含量、CBS活性;蛋白质印迹法(Westernblot)检测大鼠脑组织CBS蛋白表达.结果 染氟6个月后,与对照组比较,低氟组和高氟组大鼠均有氟斑牙出现;大鼠尿氟、骨氟组间比较差异有统计学意义(F值分别为65.16、67.93,P均＜0.05).其中尿氟和骨氟在低氟组[(5.25±0.45) mg/L,(1 196.54±72.78)mg/kg]和高氟组[(13.17±0.98)mg/L,(2 656.61±170.12)mg/kg]的水平均高于对照组[(3.64±0.20) mg/L,(870.71±71.51)mg/kg,P均＜0.05],且呈剂量依赖性增加(P均＜ 0.01).大鼠血清、脑组织中H2S含量组间比较差异有统计学意义(F值分别为4.83、1456.13,P均＜0.05).其中高氟组大鼠血清H2S[(17.64±2.38)μmol/L]高于对照组[(10.29±0.74)μmol/L,P＜0.01];低氟组和高氟组大鼠脑组织H2S[(364.74±2.06)、(513.43±4.18)μmol/L]均高于对照组[(314.94±0.72) μmol/L,P均＜0.01],且呈剂量依赖性增加(P＜0.01).大鼠脑组织中CBS活性组间比较差异有统计学意义(F=760.63,P＜ 0.01).其中低氟组和高氟组大鼠脑组织CBS活性[(438.90±2.83)、(529.83±2.37)mmol· kg-1· min-1]低于对照组[(596.33±2.75) mmol·kg-1·min-1,P均＜0.01].高氟组大鼠脑组织CBS蛋白表达(1.49±0.08)高于对照组(1.19±0.06,P＜0.05).结论 慢性氟中毒能够影响大鼠内源性CBS及H2S水平,除CBS活性外,呈剂量依赖性增加.
目的 觀察慢性氟中毒對大鼠內源性胱硫醚-β-閤成酶(CBS)及硫化氫(H2S)水平的影響.方法 48隻SD大鼠(體質量105～180 g)按體質量採用隨機數字錶法分為3組,每組16隻大鼠(雌雄各半),其中對照組、低氟組、高氟組大鼠飼料氟含量分彆為9.80、15.40、23.80 mg/kg.染氟6箇月後,氟離子選擇電極法檢測大鼠尿氟及骨氟;亞甲基藍法檢測大鼠血清及腦組織H2S含量、CBS活性;蛋白質印跡法(Westernblot)檢測大鼠腦組織CBS蛋白錶達.結果 染氟6箇月後,與對照組比較,低氟組和高氟組大鼠均有氟斑牙齣現;大鼠尿氟、骨氟組間比較差異有統計學意義(F值分彆為65.16、67.93,P均＜0.05).其中尿氟和骨氟在低氟組[(5.25±0.45) mg/L,(1 196.54±72.78)mg/kg]和高氟組[(13.17±0.98)mg/L,(2 656.61±170.12)mg/kg]的水平均高于對照組[(3.64±0.20) mg/L,(870.71±71.51)mg/kg,P均＜0.05],且呈劑量依賴性增加(P均＜ 0.01).大鼠血清、腦組織中H2S含量組間比較差異有統計學意義(F值分彆為4.83、1456.13,P均＜0.05).其中高氟組大鼠血清H2S[(17.64±2.38)μmol/L]高于對照組[(10.29±0.74)μmol/L,P＜0.01];低氟組和高氟組大鼠腦組織H2S[(364.74±2.06)、(513.43±4.18)μmol/L]均高于對照組[(314.94±0.72) μmol/L,P均＜0.01],且呈劑量依賴性增加(P＜0.01).大鼠腦組織中CBS活性組間比較差異有統計學意義(F=760.63,P＜ 0.01).其中低氟組和高氟組大鼠腦組織CBS活性[(438.90±2.83)、(529.83±2.37)mmol· kg-1· min-1]低于對照組[(596.33±2.75) mmol·kg-1·min-1,P均＜0.01].高氟組大鼠腦組織CBS蛋白錶達(1.49±0.08)高于對照組(1.19±0.06,P＜0.05).結論 慢性氟中毒能夠影響大鼠內源性CBS及H2S水平,除CBS活性外,呈劑量依賴性增加.
목적 관찰만성불중독대대서내원성광류미-β-합성매(CBS)급류화경(H2S)수평적영향.방법 48지SD대서(체질량105～180 g)안체질량채용수궤수자표법분위3조,매조16지대서(자웅각반),기중대조조、저불조、고불조대서사료불함량분별위9.80、15.40、23.80 mg/kg.염불6개월후,불리자선택전겁법검측대서뇨불급골불;아갑기람법검측대서혈청급뇌조직H2S함량、CBS활성;단백질인적법(Westernblot)검측대서뇌조직CBS단백표체.결과 염불6개월후,여대조조비교,저불조화고불조대서균유불반아출현;대서뇨불、골불조간비교차이유통계학의의(F치분별위65.16、67.93,P균＜0.05).기중뇨불화골불재저불조[(5.25±0.45) mg/L,(1 196.54±72.78)mg/kg]화고불조[(13.17±0.98)mg/L,(2 656.61±170.12)mg/kg]적수평균고우대조조[(3.64±0.20) mg/L,(870.71±71.51)mg/kg,P균＜0.05],차정제량의뢰성증가(P균＜ 0.01).대서혈청、뇌조직중H2S함량조간비교차이유통계학의의(F치분별위4.83、1456.13,P균＜0.05).기중고불조대서혈청H2S[(17.64±2.38)μmol/L]고우대조조[(10.29±0.74)μmol/L,P＜0.01];저불조화고불조대서뇌조직H2S[(364.74±2.06)、(513.43±4.18)μmol/L]균고우대조조[(314.94±0.72) μmol/L,P균＜0.01],차정제량의뢰성증가(P＜0.01).대서뇌조직중CBS활성조간비교차이유통계학의의(F=760.63,P＜ 0.01).기중저불조화고불조대서뇌조직CBS활성[(438.90±2.83)、(529.83±2.37)mmol· kg-1· min-1]저우대조조[(596.33±2.75) mmol·kg-1·min-1,P균＜0.01].고불조대서뇌조직CBS단백표체(1.49±0.08)고우대조조(1.19±0.06,P＜0.05).결론 만성불중독능구영향대서내원성CBS급H2S수평,제CBS활성외,정제량의뢰성증가.
Objective To observe the effects of fluorosis on the levels of endogenous cystathionine beta-synthase (CBS) and hydrogen sulfide (H2S) in rats.Methods According to body weight,forty-eight Sprague-Dawley rats (body weight 105-180 g) were divided into three groups by a random number table(16 rats in each group,half male).Fluorine contents of the feed in control group,low-fluoride group and high-fluoride group were 9.80,15.40 and 23.80 mg/kg.After 6 months of fluorine exposure,the fluorine contents of urine and bone were determined by the method of fluorine ion-selective electrode ; H2S levels in serum and brain and the activity of CBS in brain were detected by methylene blue; and protein expression of CBS was detected by Western blotting.Results Compared with control group,dental fluorosis was found in rats of low-fluoride and high-fluoride groups.The differences of fluorine contents of urine and femur were statistically significant between groups(F =65.16,67.93,all P ＜ 0.05).The urinary and femoral fluorine in low-fluoride groups [(5.25 ± 0.45)mg/L,(1 196.54 ± 72.78)mg/kg] and high-fluoride groups[(13.17 ± 0.98)mg/L,(2 656.61 ± 170.12)mg/kg] were higher than those of control groups [(3.64 ± 0.20)mg/L,(870.71 ± 71.51)mg/kg,all P ＜ 0.05],and the increases were in a dose-dependent fashion(all P ＜ 0.01).The differences of H2S contents in serum and brain were statistically significant(F =4.83,1 456.13,all P ＜ 0.05).The H2S content in serum was higher in high-fluoride group [(17.64 ± 2.38) μ mol/L] than that of the control group [(10.29 ± 0.74) μ mol/L,P ＜ 0.01].The H2S contents in brain were higher in the low-fluoride [(364.74 ± 2.06)μmol/L] and high-fluoride groups [(513.43 ± 4.18) μmol/L] than those of the control group[(314.94 ± 0.72)μmol/L,all P ＜ 0.01],and the increase was in a dose-dependent fashion (P ＜ 0.01).The difference of CBS activity was statistically significant between groups (F =760.63,P ＜ 0.01).The CBS activities were lower in low-fluoride [(438.90 ± 2.83) mmol· kg-1· min-1] and high-fluoride groups [(529.83 ± 2.37)mmol· kg-1· min-1] than those of the control group [(596.33 ± 2.75) mmol · kg-1· min-1,all P ＜ 0.01],whereas the protein expression of CBS in brain in high-fluoride group (1.49 ± 0.08) was higher than that of the control group (1.19 ± 0.06,P ＜ 0.05).Conclusion Chronic fluorosis can affect the levels of endogenous CBS and H2,S,and the increases are in a dose-dependent fashion in addition to CBS activity.
