中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2014年
3期
272-274
,共3页
氟%睾丸%细胞周期%细胞凋亡%生殖毒性
氟%睪汍%細胞週期%細胞凋亡%生殖毒性
불%고환%세포주기%세포조망%생식독성
Fluoride%Testis%Cell cycle%Cell apoptosis%Preconception injuries
目的 观察慢性氟中毒对雄性大鼠睾丸细胞周期及细胞凋亡的影响.方法 选择健康雄性Wistar大鼠32只,体质量150～180 g,按体质量采用随机数字表法分为4组:对照组和低、中、高氟组,分别用生理盐水和100、200、300 mg· kg-1· d-1氟化钠(NaF)灌胃90 d,每组8只,每天称体质量.实验结束后颈椎脱位法处死大鼠,摘取睾丸组织,称质量并计算脏器系数;采用流式细胞术检测睾丸细胞周期的变化及细胞凋亡.结果 染氟第30天大鼠体质量组间比较,差异有统计学意义(F=3.884,P＜0.05),其中低、中氟组[(235.00±14.56)、(235.44±24.99)g]明显高于高氟组[(206.00±18.16)g,P均＜0.05];第0、60、90天大鼠体质量组间比较,差异无统计学意义(F值分别为0.501、0.578、1.893,P均＞0.05).低、中、高氟组大鼠睾丸细胞G0/G1期所占比例[(57.60±7.26)％、(52.80±3.20)％、(73.13±4.08)％]与对照组[(43.10±3.62)％]比较,明显升高(P均＜ 0.05);低、中、高氟组大鼠睾丸细胞S期所占比例[(10.58±2.58)％、(9.35±0.35)％、(9.55±0.50)％]与对照组[(19.23±0.61)％]比较,明显降低(P均＜0.05);高氟组大鼠睾丸细胞G2/M期所占比例[(17.18±2.21)％]与对照组[(36.34±5.05)％]比较,明显降低(P＜0.05).中、高氟组睾丸细胞凋亡率[(71.03±2.30)％、(71.90±2.16)％]与对照组[(60.80±2.34)％]比较,明显升高(P均＜0.05).结论 慢性氟中毒可导致睾丸生殖细胞周期紊乱、诱导生殖细胞凋亡,损害大鼠生殖系统.
目的 觀察慢性氟中毒對雄性大鼠睪汍細胞週期及細胞凋亡的影響.方法 選擇健康雄性Wistar大鼠32隻,體質量150～180 g,按體質量採用隨機數字錶法分為4組:對照組和低、中、高氟組,分彆用生理鹽水和100、200、300 mg· kg-1· d-1氟化鈉(NaF)灌胃90 d,每組8隻,每天稱體質量.實驗結束後頸椎脫位法處死大鼠,摘取睪汍組織,稱質量併計算髒器繫數;採用流式細胞術檢測睪汍細胞週期的變化及細胞凋亡.結果 染氟第30天大鼠體質量組間比較,差異有統計學意義(F=3.884,P＜0.05),其中低、中氟組[(235.00±14.56)、(235.44±24.99)g]明顯高于高氟組[(206.00±18.16)g,P均＜0.05];第0、60、90天大鼠體質量組間比較,差異無統計學意義(F值分彆為0.501、0.578、1.893,P均＞0.05).低、中、高氟組大鼠睪汍細胞G0/G1期所佔比例[(57.60±7.26)％、(52.80±3.20)％、(73.13±4.08)％]與對照組[(43.10±3.62)％]比較,明顯升高(P均＜ 0.05);低、中、高氟組大鼠睪汍細胞S期所佔比例[(10.58±2.58)％、(9.35±0.35)％、(9.55±0.50)％]與對照組[(19.23±0.61)％]比較,明顯降低(P均＜0.05);高氟組大鼠睪汍細胞G2/M期所佔比例[(17.18±2.21)％]與對照組[(36.34±5.05)％]比較,明顯降低(P＜0.05).中、高氟組睪汍細胞凋亡率[(71.03±2.30)％、(71.90±2.16)％]與對照組[(60.80±2.34)％]比較,明顯升高(P均＜0.05).結論 慢性氟中毒可導緻睪汍生殖細胞週期紊亂、誘導生殖細胞凋亡,損害大鼠生殖繫統.
목적 관찰만성불중독대웅성대서고환세포주기급세포조망적영향.방법 선택건강웅성Wistar대서32지,체질량150～180 g,안체질량채용수궤수자표법분위4조:대조조화저、중、고불조,분별용생리염수화100、200、300 mg· kg-1· d-1불화납(NaF)관위90 d,매조8지,매천칭체질량.실험결속후경추탈위법처사대서,적취고환조직,칭질량병계산장기계수;채용류식세포술검측고환세포주기적변화급세포조망.결과 염불제30천대서체질량조간비교,차이유통계학의의(F=3.884,P＜0.05),기중저、중불조[(235.00±14.56)、(235.44±24.99)g]명현고우고불조[(206.00±18.16)g,P균＜0.05];제0、60、90천대서체질량조간비교,차이무통계학의의(F치분별위0.501、0.578、1.893,P균＞0.05).저、중、고불조대서고환세포G0/G1기소점비례[(57.60±7.26)％、(52.80±3.20)％、(73.13±4.08)％]여대조조[(43.10±3.62)％]비교,명현승고(P균＜ 0.05);저、중、고불조대서고환세포S기소점비례[(10.58±2.58)％、(9.35±0.35)％、(9.55±0.50)％]여대조조[(19.23±0.61)％]비교,명현강저(P균＜0.05);고불조대서고환세포G2/M기소점비례[(17.18±2.21)％]여대조조[(36.34±5.05)％]비교,명현강저(P＜0.05).중、고불조고환세포조망솔[(71.03±2.30)％、(71.90±2.16)％]여대조조[(60.80±2.34)％]비교,명현승고(P균＜0.05).결론 만성불중독가도치고환생식세포주기문란、유도생식세포조망,손해대서생식계통.
Objective To observe the effects of fluoride on testicular cell cycle and cell apoptosis of male rats.Methods Thirty-two healthy male Wistar rats,weighting 150-180 g,were randomly divided into 4 groups by body weight using random number table,normal sodium (control),the low-dose,medium-dose and high-dose groups(100,200,300 mg· kg-1· d-1 NaF,respectively) by intragastric administration for 90 days,and bodyweight was observed daily.After the last intragastric administration,all rats were killed by cervical dislocation.The testicular cell cycle and cell apoptosis were measured by flow cytometry.Results After 30 days exposure,the difference of body weight between groups was statistically significant(F =3.884,P ＜ 0.05).The body weights in low-and medium-dose groups[(235.00 ± 14.56),(235.44 ± 24.99)g] were significantly increased than that of high-dose group [(206.00 ± 18.16)g,all P ＜ 0.05].There was no significant difference of body weight between the groups at 0,60 and 90 days(F =0.501,0.578,1.893,all P ＞ 0.05).Compared with the control group[(43.10 ± 3.62)％],the percentages of G0/G1 stage cells were significantly increased in all the NaF-treated groups [(57.60 ± 7.26) ％,(52.80 ± 3.20) ％,(73.13 ± 4.08) ％] and the percentages of S stage cells were significantly decreased in all the NaF-treated groups [(10.58 ± 2.58)％,(9.35 ± 0.35)％,(9.55 ± 0.50)％] compared to the control group[(19.23 ± 0.61)％,all P ＜ 0.05].On the other hand,the percentage of G2/M stage cells decreased significantly in high-dose group[(17.18 ± 2.21)％] compared with the control group[(36.34 ± 5.05)％,P ＜ 0.05].The testicular cell apoptosis ratios in all the NaF-treated groups were higher than that in the control group,but only in medium-and high-dose groups[(71.03 ± 2.30)％,(71.90 ± 2.16)％],the difference was statistically significant compared with the control group [(60.80 ± 2.34)％,all P ＜ 0.05].Conclusion Chronic fluorosis can change testicular cell cycle and cell apoptosis and damage the reproductive system.
