CAJ | 학술논문

目的了解中国鼠疫耶尔森菌不同分离株之间的染色体结构差异,探索染色体重排在鼠疫菌中发生的原因及用于菌株遗传特征识别和亲缘关系分析的可能性.方法以完成全基因组测序的首株鼠疫菌株CO92(美国)为参考株,以从中国分离出的已完成全基因组测序的4株鼠疫菌株(内蒙古91001、云南剑川D182038、云南玉龙D106004、西藏那曲Z176003)为对比株,根据美国国立生物技术信息中心网站(http://www.ncbi.nlm.nih.gov/genome)上公布的这5株鼠疫菌的染色体序列及染色体上所有编码基因序列间的相似性,将鼠疫菌染色体人为的划分成多个大的DNA节段(染色体板块),确定这些板块在不同鼠疫菌株间排列方式的差异以及板块之间的断裂区片段的基因组成;根据菌株两两比较所得的重排差异结果,分析菌株间的亲缘关系.结果除Z176003菌株外,CO92、D182038、D106004、91001菌株可被分成44个相对独立的染色体板块,板块内部基因组成和排列高度稳定,板块之间可以相对移动.与参考株CO92菌株比较,D182038、D106004菌株染色体均存在13处重排,Z176003菌株染色体存在14处重排,91001菌株染色体存在37处板块排列顺序的改变.菌株间两两比较,D106004与Z176003菌株之间仅有8处染色体板块排列方式不同,表明二者的亲缘关系最为接近.CO92菌株染色体上的相邻板块之间存在43个断裂区,有39个断裂区的DNA片段含有插入序列,其中25个插入序列为IS100.结论鼠疫菌基因组具有一定的流动性,不同菌株间的染色体结构存在较大差异,染色体重排事件发生的原因与其拥有的插入序列密切相关.菌株间染色体的重排差异,能用于菌株遗传特征的识别和亲缘远近关系的分析.
목적 료해중국서역야이삼균불동분리주지간적염색체결구차이,탐색염색체중배재서역균중발생적원인급용우균주유전특정식별화친연관계분석적가능성.방법 이완성전기인조측서적수주서역균주CO92(미국)위삼고주,이종중국분리출적이완성전기인조측서적4주서역균주(내몽고91001、운남검천D182038、운남옥룡D106004、서장나곡Z176003)위대비주,근거미국국립생물기술신식중심망참(http://www.ncbi.nlm.nih.gov/genome)상공포적저5주서역균적염색체서렬급염색체상소유편마기인서렬간적상사성,장서역균염색체인위적화분성다개대적DNA절단(염색체판괴),학정저사판괴재불동서역균주간배렬방식적차이이급판괴지간적단렬구편단적기인조성;근거균주량량비교소득적중배차이결과,분석균주간적친연관계.결과 제Z176003균주외,CO92、D182038、D106004、91001균주가피분성44개상대독립적염색체판괴,판괴내부기인조성화배렬고도은정,판괴지간가이상대이동.여삼고주CO92균주비교,D182038、D106004균주염색체균존재13처중배,Z176003균주염색체존재14처중배,91001균주염색체존재37처판괴배렬순서적개변.균주간량량비교,D106004여Z176003균주지간부유8처염색체판괴배렬방식불동,표명이자적친연관계최위접근.CO92균주염색체상적상린판괴지간존재43개단렬구,유39개단렬구적DNA편단함유삽입서렬,기중25개삽입서렬위IS100.결론 서역균기인조구유일정적류동성,불동균주간적염색체결구존재교대차이,염색체중배사건발생적원인여기옹유적삽입서렬밀절상관.균주간염색체적중배차이,능용우균주유전특정적식별화친연원근관계적분석.
Objective To study the differences of chromosomal structure among Yersinia pestis strains isolated from China,and to investigate the reasons of chromosomal rearrangement events occurred in Yersinia pestis as well as the possibility of strain identification and phylogenetic analysis based on the chromosomal rearrangement features.Methods According to the genome sequence data downloaded from web of National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov/genome),alignment of all the coding sequences (CDSs) among five strains(American strain CO92 as reference and other four completely sequenced strains from Inner Mongolia,Jianchuan of Yunnan,Yulong of Yunnan,Naqu of Tibet in China named 91001,D182038,D106004 and Z176003 as comparison strains) was performed,and then the chromosome of Yersinia pestis was divided into several large DNA segments (named chromosomal plate in the text) according to the similarity of CDSs.Plate arrangement patterns in each strain' s chromosome and gene content of breakpoint regions were determined.Finally,genetic relationships among Yersinia pestis strains were analyzed on the basis of rearrangement diversity from paired-comparison.Results Yersinia pestis chromosomes of strains CO92,D182038,D106004,91001 were composed of 44 relatively independent plates,except strain Z176003.Gene order was very stable within each plate,while it was movable between the plates.Comparing with the reference strain CO92,13 rearrangement events occurred in the chromosomes of both strain D182038 and strain D106004,and 14 rearrangement events involved in Z176003,while 37 rearrangement events occurred in 91001.Paired-comparison data showed that only 8 plates order differences were existed between D106004 and Z176003.Forty-three breakpoint regions were identified on the chromosome of strain CO92,and 39 of them contained insertion sequences,and 25 of them were IS100.Conclusions Yersinia pestis genome represents a high degree of genetic flux,and chromosomal structures of strains are significantly different from each other.Chromosomal rearrangement events is closely related to the large number of insertion sequences in the Yersinia pestis chromosome.Rearrangement diversity among Yersinia pestis strains could reflect their genetic relationships.