CAJ | 학술논문

目的探讨组织蛋白酶S(Cathepsin S)在人腹主动脉瘤(AAA)组织和血管紧张素Ⅱ(AngⅡ)灌注载脂蛋白E基因缺陷小鼠(ApoE-/-)引起AAA病变中的表达.方法收集符合临床诊断的腹主动脉瘤患者标本(AAA组),以正常血管为对照(对照组)；AngⅡ以1000ng·kg-1 ·d-1的剂量持续灌注ApoE-/-小鼠28 d建立小鼠AAA模型,以0.9％氯化钠注射液灌注作为对照.弹力纤维染色观察人和小鼠AAA病变组织弹力纤维断裂,免疫组化观察人和小鼠AAA病变组织Cathepsin S的蛋白表达.结果弹力纤维染色显示,人和小鼠AAA组织的弹力纤维断裂显著增加(P＜0.05)；免疫组化结果显示人和小鼠AAA组织的Cathepsin S表达较对照组显著增加(P＜0.05).结论 Cathepsin S在人和小鼠AAA病变组织中被激活,可能参与AAA的发病过程.
목적 탐토조직단백매S(Cathepsin S)재인복주동맥류(AAA)조직화혈관긴장소Ⅱ(AngⅡ)관주재지단백E기인결함소서(ApoE-/-)인기AAA병변중적표체.방법 수집부합림상진단적복주동맥류환자표본(AAA조),이정상혈관위대조(대조조)；AngⅡ이1000ng·kg-1 ·d-1적제량지속관주ApoE-/-소서28 d건립소서AAA모형,이0.9％록화납주사액관주작위대조.탄력섬유염색관찰인화소서AAA병변조직탄력섬유단렬,면역조화관찰인화소서AAA병변조직Cathepsin S적단백표체.결과 탄력섬유염색현시,인화소서AAA조직적탄력섬유단렬현저증가(P＜0.05)；면역조화결과현시인화소서AAA조직적Cathepsin S표체교대조조현저증가(P＜0.05).결론 Cathepsin S재인화소서AAA병변조직중피격활,가능삼여AAA적발병과정.
Objective To observe the expression of Cathepsin S in the human abdominal aortic aneurysm (AAA) and angiotensin Ⅱ(AngⅡ) perfusion induced AAA lesions in apolipoprotein E-deficient(ApoE-/-) mice.Methods AAA specimens of clinical diagnosis patients(AAA group) and the normal blood vessels(control group)were collected.The AAA model was established by infused Ang Ⅱ 1 000ng · kg-1 · d-1 to ApoE-/-mice.Elastic fibersfracture was observed by elastic fiber staining and Cathepsin S expression by immunohistochemistry.Results The elastic fiber staining revealed that the elastic fibers fracture of the human and mouse AAA group increased significantly (P ＜ 0.05) ; Cathepsin S expression significantly increased in the human and mouse AAA group than the control group by immunohistochemistry(P ＜ 0.05).Conclusion Cathepsin S is activated in human and murine AAA lesions and may be involved in the pathogenesis of AAA.