CAJ | 학술논문

目的探讨蛛网膜下腔出血(SAH)后白细胞介素-1β(IL-1β)的表达变化以及与脑水肿、脑血管痉挛的关系.方法通过大脑前动脉穿刺法制作大鼠SAH模型,用干重-湿重法测定蛛网膜下腔出血后脑水肿的变化情况,HE染色观察基底动脉的血管形态学变化,Westernblot实验检测大鼠脑组织IL-1β的表达情况.结果 SAH后大鼠脑组织水肿从1d开始,7d时最明显,以后逐渐减轻.IL-1β蛋白水平逐渐增高,术后7d达高峰,之后逐渐降低,14 d时仍维持较高水平.IL-1β水平和脑水肿的变化具有相关性,而与血管痉挛的程度没有相关性.结论大鼠蛛网膜下腔出血后IL-1β水平增高,7d达到高峰；脑组织IL-1β表达变化与脑水肿程度呈正相关,表明IL-1β可能参与蛛网膜下腔出血后脑水肿的形成.
목적 탐토주망막하강출혈(SAH)후백세포개소-1β(IL-1β)적표체변화이급여뇌수종、뇌혈관경련적관계.방법 통과대뇌전동맥천자법제작대서SAH모형,용간중-습중법측정주망막하강출혈후뇌수종적변화정황,HE염색관찰기저동맥적혈관형태학변화,Westernblot실험검측대서뇌조직IL-1β적표체정황.결과 SAH후대서뇌조직수종종1d개시,7d시최명현,이후축점감경.IL-1β단백수평축점증고,술후7d체고봉,지후축점강저,14 d시잉유지교고수평.IL-1β수평화뇌수종적변화구유상관성,이여혈관경련적정도몰유상관성.결론 대서주망막하강출혈후IL-1β수평증고,7d체도고봉；뇌조직IL-1β표체변화여뇌수종정도정정상관,표명IL-1β가능삼여주망막하강출혈후뇌수종적형성.
Objective To investigate the changes of interleukin-1β (IL-1β) in rat brain after subarachnoid hemorhage (SAH) and its relation to cerebral edema and vasospasm.Methods Subarachnoid hemorrhage rat model was produced by anterior cerebral artery perforation method.The brain water content was measured by dry-wet weight method.Hematoxylin-eosin (HE) staining was used to assess vasospasm in the basilar artery,and the expression of IL-1β after SAH was examined by Western blot.Results After SAH,the cerebral edema gradually occured from 1 d,reached the peak at 7d.The level of IL-1β protein started to increase in 1 d,reached its peak in 7d,and remained at a relative high level in 14d.There's correlation between the level of IL-1β protein and changes of cerebral edema.Conclusion After SAH,The level of IL-1β gradually rised and fits remarkably well with the time course of cerebral edema,which may indicate that IL-1β leads to the development of cerebral edema after SAH.