CAJ | 학술논문

目的探索建立非人灵长目动物-食蟹猴的肾移植模型,总结模型建立中需要注意的问题.方法气管插管麻醉下,取配型良好雄性食蟹猴50只行同种异体肾移植,将供肾动脉、肾静脉分别与受体腹主动脉、下腔静脉行端侧吻合术,尿路重建采用输尿管-膀胱吻合法.术后常规抗排斥治疗及抗炎治疗.结果 50只肾移植食蟹猴中1只因手术过程中麻醉意外死亡；有7只术后出现并发症,且均伴有肌酐值的突然升高,行移植肾及血管超声检查,其中2只肾静脉血栓形成,5只出现移植肾输尿管尿漏.所有动物模型无手术感染出现,抗排斥治疗下存活良好,血肌酐及尿量正常.结论应用非人灵长目动物-食蟹猴建立肾移植模型方法可靠,但应注意手术技术的训练,术后并发症的预防.应用此模型进行肾移植免疫耐受、异种肾移植研究等可为临床试验提供更为准确可靠的依据.
목적 탐색건립비인령장목동물-식해후적신이식모형,총결모형건립중수요주의적문제.방법 기관삽관마취하,취배형량호웅성식해후50지행동충이체신이식,장공신동맥、신정맥분별여수체복주동맥、하강정맥행단측문합술,뇨로중건채용수뇨관-방광문합법.술후상규항배척치료급항염치료.결과 50지신이식식해후중1지인수술과정중마취의외사망；유7지술후출현병발증,차균반유기항치적돌연승고,행이식신급혈관초성검사,기중2지신정맥혈전형성,5지출현이식신수뇨관뇨루.소유동물모형무수술감염출현,항배척치료하존활량호,혈기항급뇨량정상.결론 응용비인령장목동물-식해후건립신이식모형방법가고,단응주의수술기술적훈련,술후병발증적예방.응용차모형진행신이식면역내수、이충신이식연구등가위림상시험제공경위준학가고적의거.
Objective To explore renal transplantation model in non-human primate cynomolgus monkeys.Methods 50 non-human primates' kidneys were transplanted into the lower part of the abdomen with end-to-side anastomosis of renal artery to aorta and renal vein to inferior vena eava,and with end-to-end anastomosis of ureter to bladder.Results In the 50 cases,1 case death as accident of anesthesia;7 cases with postoperative complications,and all with creatinine sudden rise,after ultrasonic examinations showed that 2 cases with renal vein thrombosis,and 5 cases appeared urinary leakage.All animal models were without surgical infections,and with normal serum creatinine,urine output.Conclusion Non-human primate animal kidney transplantation model establishment method is reliable,but should pay attention to the the surgical technique training,complications prevention.The model is valuable for application in the research of immune tolerance,heterogeneous transplant.